LOCUS BC060787 2724 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens holocarboxylase synthetase
(biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing))
ligase), mRNA (cDNA clone MGC:71574 IMAGE:30343493), complete cds.
ACCESSION BC060787
VERSION BC060787.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2724)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2724)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-NOV-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Stefan Hansson
cDNA Library Preparation: Michael Brownstein / Ted Usdin
Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 133 Row: f Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 46255044.
FEATURES Location/Qualifiers
source 1..2724
/db_xref="H-InvDB:HIT000260089"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:71574 IMAGE:30343493"
/tissue_type="Placenta, normal"
/clone_lib="NIH_MGC_147"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..2724
/gene="HLCS"
/gene_synonym="HCS"
/db_xref="GeneID:3141"
/db_xref="HGNC:HGNC:4976"
/db_xref="MIM:609018"
CDS 439..2619
/gene="HLCS"
/gene_synonym="HCS"
/codon_start=1
/product="holocarboxylase synthetase
(biotin-(proprionyl-Coenzyme A-carboxylase
(ATP-hydrolysing)) ligase)"
/protein_id="AAH60787.1"
/db_xref="GeneID:3141"
/db_xref="HGNC:HGNC:4976"
/db_xref="MIM:609018"
/translation="MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPK
PEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLH
LSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGK
APNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLV
IATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKAD
QSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVH
LELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAA
EEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEH
FNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGR
GGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRV
KWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQH
KAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGP
KVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR"
BASE COUNT 682 a 672 c 718 g 652 t
ORIGIN
1 gcgggtgacc acccggcgca ggagcggccg cgtctcggcc tcagaatcca ttgaagactt
61 gaacaagtgg gccctatttc ttgtgtctcc ttttatactt gaagcagaac acatagcatt
121 tgtgacggag agcatttggg tacaaagtga gaatttacag agatcatcct cttcagaaac
181 agttcggttt ttgcccacta gggatgatgt ggtttctcat gaggttactt gctctaaagg
241 actttatatt ttggaaccat aagagcaccc ttgtggccca ggcactttat ggatgatccc
301 ttttagtgct cccagtaacc ttccaagatt gtcaagtggt cagactgttg tttgccatta
361 gcttgcagac ctggggatcc ttatcggcta attgctgaag caagtgtgga caacttcagc
421 aagctggggg tggcgttcat ggaagataga ctccacatgg ataatggact ggtaccccaa
481 aagattgtgt cggtgcactt gcaggactcc actctgaagg aagttaagga tcaggtctca
541 aacaagcaag cccagatcct agagccgaag cctgaacctt ctcttgagat taagcctgag
601 caggacggta tggagcatgt tggcagagat gacccaaagg ctcttggtga agaacccaaa
661 caaaggagag gcagtgcctc tgggagtgag cctgctgggg acagtgacag gggagggggc
721 cccgttgagc attatcacct ccatctgtct agttgccacg agtgtctgga acttgagaac
781 agcaccattg agtcagtcaa gtttgcgtct gccgagaaca ttccagacct tccctacgat
841 tatagcagca gtttggagag tgttgctgat gagacctccc ccgaaagaga agggaggaga
901 gtcaacctca cgggaaaggc acccaacatc ctcctctatg tgggctccga ctcccaggaa
961 gccctcggcc ggttccacga ggtccggtct gtgctggccg actgtgtgga cattgacagt
1021 tatattctct accacctgct ggaggacagt gctctcagag acccgtggac ggacaactgt
1081 ctgctgttgg tcattgctac cagggagtcc attcccgaag acctgtacca gaagttcatg
1141 gcctatcttt ctcagggagg gaaggtgttg ggcctgtctt catccttcac ctttggtggc
1201 tttcaggtga caagcaaggg tgcactgcac aagacagtcc agaacttggt tttctccaag
1261 gctgaccaga gtgaggtgaa gctcagcgtc ttgagcagtg gctgcaggta ccaggaaggc
1321 cccgtccggc tcagccccgg caggctccag ggccacctgg agaatgagga caaggacagg
1381 atgattgtgc atgtgccttt tggaactcgc gggggagaag ctgttctttg ccaggtgcac
1441 ttagaactac ctcccagctc caacatagtg caaactccag aagattttaa cttgctcaag
1501 tcaagcaatt ttagaagata cgaagtcctt agagagattc tgacaaccct tggcctcagc
1561 tgtgacatga aacaagttcc tgccttaact cctctttact tgctgtcagc tgcggaggaa
1621 atcagggatc ctcttatgca gtggcttggg aaacatgtgg actccgaggg agaaataaaa
1681 tccggccagc tctctcttag atttgtttca tcctacgtgt ctgaagtaga aataacccca
1741 tcttgtatac ctgtggtgac caacatggag gccttctcat cagaacattt caacttagag
1801 atctatcgcc aaaatctgca gaccaagcag ttggggaaag taattttgtt tgccgaagtg
1861 acccccacaa cgatgcgtct cctggatggg ctgatgtttc agacaccgca ggaaatgggc
1921 ttaatagtga tcgcggcccg gcagaccgag ggcaaaggac ggggagggaa tgtgtggctg
1981 agccctgtgg gatgtgctct ttctactctg ctcatctcca ttccactgag atcccagctg
2041 ggacagagga tcccgtttgt ccagcatctg atgtccgtgg ctgtcgtgga agcagtgagg
2101 tccattcccg agtatcagga tatcaactta cgagtgaagt ggcccaacga tatttattac
2161 agtgacctca tgaagatcgg cggagttctg gttaactcaa cactcatggg agaaacattt
2221 tatatactta ttggctgtgg atttaatgtg actaacagta accctaccat ctgcatcaac
2281 gacctcatca cagaatacaa taaacaacac aaggcagaac tgaagccctt aagagccgat
2341 tatctcatcg ccagagtcgt gactgtgctg gagaaactga tcaaagagtt tcaggacaaa
2401 gggcccaaca gcgtccttcc cctttattac cgatactggg tccacagtgg tcagcaagtc
2461 catctgggca gcgcagaggg accaaaggtg tccatcgttg gcctggacga ttctggcttc
2521 ctccaggttc accaggaggg cggcgaggtt gtgactgtgc acccggacgg caactccttc
2581 gacatgctga gaaacctcat cctccccaaa cggcggtaat gccgggcgtc cccgagacgc
2641 ggctgcctgt ccgtgcccat gcatctggaa atctaattta gagttgtagg tgaattttct
2701 tttcctccaa aaaaaaaaaa aaaa
//