LOCUS BC060781 3148 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens ras homolog gene family, member T1, mRNA (cDNA clone
MGC:71555 IMAGE:4823115), complete cds.
ACCESSION BC060781
VERSION BC060781.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3148)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3148)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-NOV-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 133 Row: l Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361737.
FEATURES Location/Qualifiers
source 1..3148
/db_xref="H-InvDB:HIT000260083"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:71555 IMAGE:4823115"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3148
/gene="RHOT1"
/gene_synonym="MIRO-1"
/db_xref="GeneID:55288"
/db_xref="HGNC:HGNC:21168"
CDS 240..2096
/gene="RHOT1"
/gene_synonym="MIRO-1"
/codon_start=1
/product="ras homolog gene family, member T1"
/protein_id="AAH60781.2"
/db_xref="GeneID:55288"
/db_xref="HGNC:HGNC:21168"
/translation="MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPA
DVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLIN
ERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFY
YAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFN
TPLAPQALEDVKNVVRKHISDGVADSGFTLKGFLFLHTLFIQRGRHETTWTVLRRFGY
DDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKGLFK
VFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQ
ESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIR
EDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCA
RIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPS
KDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR"
BASE COUNT 952 a 618 c 671 g 907 t
ORIGIN
1 gccgccgccg ccgccgccgc cgccgccgcc gccgccgccg ccgccgccac agcccgctgg
61 gccggaggag gcggagctgg cgctgtcccg gctctcttgc ggggaagcaa ctgagggggc
121 ggcgcggcgg gccccggcgg ccgaagaggc tggcaggtgg cgccgtgggg tgggtgctcc
181 tggtgagagg agtccactcc gtgcgtgcgg gcggaggccg gcccccgaga gccgccgaca
241 tgaagaaaga cgtgcggatc ctgctggtgg gagaacctag agttgggaag acatcactga
301 ttatgtctct ggtcagtgaa gaatttccag aagaggttcc tccccgggca gaagaaatca
361 ccattccagc tgatgtcacc ccagagagag ttccaacaca cattgtagat tactcagaag
421 cagaacagag tgatgaacaa cttcatcaag aaatatctca ggctaatgtc atctgtatag
481 tgtatgccgt taacaacaag cattctattg ataaggtaac aagtcgatgg attcctctca
541 taaatgaaag aacagacaaa gacagcaggc tgcctttaat attggttggg aacaaatctg
601 atctggtgga atatagtagt atggagacca tccttcctat tatgaaccag tatacagaaa
661 tagaaacctg tgtggagtgt tcagcgaaaa acctgaagaa catatcagag ctcttttatt
721 acgcacagaa agctgttctt catcctacag ggcccctgta ctgcccagag gagaaggaga
781 tgaaaccagc ttgtataaaa gcccttactc gtatatttaa aatatctgat caagataatg
841 atggtactct caatgatgct gaactcaact tctttcagag gatttgtttc aacactccat
901 tagctcctca agctctggag gatgtcaaga atgtagtcag aaaacatata agtgatggtg
961 tggctgacag tgggtttacc ctgaaaggtt ttctcttttt acacacactt tttatccaga
1021 gagggagaca cgaaactact tggactgtgc ttcgacgatt tggttatgat gatgacctgg
1081 atttgacacc tgaatatttg ttccccctgc tgaaaatacc tcctgattgc actactgaat
1141 taaatcatca tgcatattta tttctccaaa gcacctttga caagcatgat ttggatagag
1201 actgtgcttt gtcacctgat gagcttaaag gtttatttaa agttttccct tacatacctt
1261 gggggccaga tgtgaataac acagtttgta ccaatgaaag aggctggata acctaccagg
1321 gattcctttc ccagtggacg ctcacgactt atttagatgt acagcggtgc ctggaatatt
1381 tgggctatct aggctattca atattgactg agcaagagtc tcaagcttca gctgttacag
1441 tgacaagaga taaaaagata gacctgcaga aaaaacaaac tcaaagaaat gtgttcagat
1501 gtaatgtaat tggagtgaaa aactgtggga aaagtggagt tcttcaggct cttcttggaa
1561 gaaacttaat gaggcagaag aaaattcgtg aagatcataa atcctactat gcgattaaca
1621 ctgtttatgt atatggacaa gagaaatact tgttgttgca tgatatctca gaatcggaat
1681 ttctaactga agctgaaatc atttgtgatg ttgtatgcct ggtatatgat gtcagcaatc
1741 ccaaatcctt tgaatactgt gccaggattt ttaagcaaca ctttatggac agcagaatac
1801 cttgcttaat cgtagctgca aagtcagacc tgcatgaagt taaacaagaa tacagtattt
1861 cacctactga tttctgcagg aaacacaaaa tgcctccacc acaagccttc acttgcaata
1921 ctgctgatgc ccccagtaag gatatctttg ttaaattgac aacaatggcc atgtatccgc
1981 acgtgacaca agctgacctc aagagctcca cgttttggct tcgagcaagt tttggtgcta
2041 ctgtttttgc agttttgggc tttgctatgt acaaagcatt attgaaacag cgatgatata
2101 aaaagaaata ctgtccctac caaaaacaaa tacttttatg tacattctga atgctttaag
2161 ttctgctaga attattgaga tatttataca tgcagagtta ctttattaat atttgtaatt
2221 catgcataag agtattttaa tgatagttat aactgcagta ttggctagca tatggaaaga
2281 aaacagctaa cagccaaact aaaatggcta aattccagag gccaaaaggg aatattttgt
2341 aaatatatgt acatattcag gcaagatatg gtctcccaag ctgagttcta gaaatgatgt
2401 ttctagacat ttctaagtgg tattgttagt gctcacttgg ctcactcttc taggtttaag
2461 ttagcccaga gattgtattt actcatggat cactttattt atttcacatt tactcagaat
2521 gatcctttgg gttctataag gacataaggt acaatttgcc attgtctctc catttttaaa
2581 aacatacaag tcagtgtcag cttaccaaca tgacattttt tcagtcagtt gtggtaggcc
2641 agccttgaag ccatcgcaca gtctagaaac ttgtgtagct gagtgtgcag ctcaccttta
2701 agggtgaagt taggtaaaag caattagcag aggcgttatc tatgtgatta tgttgcttcc
2761 ttgtcagtat gttgaatttt atagcccttt caatgaaata aaaaaaaaat ttgtatatta
2821 ccaatgtttt tagtttaaat aaagagtcac ccttactact gttgaatttc atcccaagtg
2881 taaatcattc tataatggct gtgtctgtta tagtatatta cagtaactgc atgtgtcacc
2941 aagtgttcta tatcaggcta ggataaccta gaggcagtaa ttttttaaat gataaaataa
3001 atctaatgaa tataaactct catgataaac ctattttttc catcatcagc cttttcaagt
3061 atttaaataa ataactgctg tgtactgtga tcttgagttc ttttgtcatc taaagtaaat
3121 atttctgtac agaaaaaaaa aaaaaaaa
//