LOCUS BC060764 3841 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens mitogen-activated protein kinase kinase 4, mRNA (cDNA
clone MGC:71508 IMAGE:5294838), complete cds.
ACCESSION BC060764
VERSION BC060764.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3841)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3841)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-NOV-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 133 Row: o Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 24497520.
FEATURES Location/Qualifiers
source 1..3841
/db_xref="H-InvDB:HIT000260066"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:71508 IMAGE:5294838"
/tissue_type="Brain, hypothalamus"
/clone_lib="NIH_MGC_96"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3841
/gene="MAP2K4"
/gene_synonym="JNKK"
/gene_synonym="JNKK1"
/gene_synonym="MAPKK4"
/gene_synonym="MEK4"
/gene_synonym="MKK4"
/gene_synonym="PRKMK4"
/gene_synonym="SEK1"
/db_xref="GeneID:6416"
/db_xref="HGNC:HGNC:6844"
/db_xref="MIM:601335"
CDS 64..1263
/gene="MAP2K4"
/gene_synonym="JNKK"
/gene_synonym="JNKK1"
/gene_synonym="MAPKK4"
/gene_synonym="MEK4"
/gene_synonym="MKK4"
/gene_synonym="PRKMK4"
/gene_synonym="SEK1"
/codon_start=1
/product="mitogen-activated protein kinase kinase 4"
/protein_id="AAH60764.1"
/db_xref="GeneID:6416"
/db_xref="HGNC:HGNC:6844"
/db_xref="MIM:601335"
/translation="MAAPSPSGGGGSGGGRGSGTPGPVGSPAPGHPAVSSMQGKRKAL
KLNFANPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDL
KDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPY
IVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNH
LKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERID
PSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEER
EFSPSFINFVNLCLTKDESKRLKYKELLKHPFILMYEERAVEVACYVCKILDQMPATP
SSPMYVD"
BASE COUNT 1133 a 798 c 808 g 1102 t
ORIGIN
1 gcgagaggcc gagcttgctg cattgcagcc gccgcggcgc cgctcggctc ttcactccca
61 acaatggcgg ctccgagccc gagcggcggc ggcggctccg ggggcggccg cggcagcggc
121 acccccggcc ccgtagggtc cccggcgcca ggccacccgg ccgtcagcag catgcagggt
181 aaacgcaaag cactgaagtt gaattttgca aatccacctt tcaaatctac agcaaggttt
241 actctgaatc ccaatcctac aggagttcaa aacccacaca tagagagact gagaacacac
301 agcattgagt catcaggaaa actgaagatc tcccctgaac aacactggga tttcactgca
361 gaggacttga aagaccttgg agaaattgga cgaggagctt atggttctgt caacaaaatg
421 gtccacaaac caagtgggca aataatggca gttaaaagaa ttcggtcaac agtggatgaa
481 aaagaacaaa aacaacttct tatggatttg gatgtagtaa tgcggagtag tgattgccca
541 tacattgttc agttttatgg tgcactcttc agagagggtg actgttggat ctgtatggaa
601 ctcatgtcta cctcgtttga taagttttac aaatatgtat atagtgtatt agatgatgtt
661 attccagaag aaattttagg caaaatcact ttagcaactg tgaaagcact aaaccactta
721 aaagaaaact tgaaaattat tcacagagat atcaaacctt ccaatattct tctggacaga
781 agtggaaata ttaagctctg tgacttcggc atcagtggac agcttgtgga ctctattgcc
841 aagacaagag atgctggctg taggccatac atggcacctg aaagaataga cccaagcgca
901 tcacgacaag gatatgatgt ccgctctgat gtctggagtt tggggatcac attgtatgag
961 ttggccacag gccgatttcc ttatccaaag tggaatagtg tatttgatca actaacacaa
1021 gtcgtgaaag gagatcctcc gcagctgagt aattctgagg aaagggaatt ctccccgagt
1081 ttcatcaact ttgtcaactt gtgccttacg aaggatgaat ccaaaaggct aaagtataaa
1141 gagcttctga aacatccctt tattttgatg tatgaagaac gtgccgttga ggtcgcatgc
1201 tatgtttgta aaatcctgga tcaaatgcca gctactccca gctctcccat gtatgtcgat
1261 tgatatcgct gctacatcag actctagaaa aaagggctga gaggaagcaa gacgtaaaga
1321 attttcatcc cgtatcacag tgtttttatt gctcgcccag acaccatgtg caataagatt
1381 ggtgttcgtt tccatcatgt ctgtatactc ctgtcaccta gaacgtgcat ccttgtaata
1441 cctgattgat cacacagtgt tagtgctggt cagagagacc tcatcctgct cttttgtgat
1501 gaacatattc atgaaatgtg gaagtcagta cgatcaagtt gttgactgtg attagatcac
1561 atcttaaatt catttctaga ctcaaaacct ggagatgcag ctactggaat ggtgttttgt
1621 cagacttcca aatcctggaa ggacacagtg atgaatgtac tatgtctgaa catagaaact
1681 cgggcttgag tgagaagagc ttgcacagcc aacgagacac attgccttct ggagctggga
1741 gacaaaggag gaatttactt tcttcaccaa gtgcaataga ttactgatgt gatattctgt
1801 tgctttacag ttacagttga tgtttgggga tcgatgtgct cagccaaatt tcctgtttga
1861 aatatcatgt taaattagaa tgaatttatc tttaccaaaa accatgttgc gttcaaagag
1921 gtgaacatta aaatatagag acaggacaga atgtgttctt ttctccttta ccagtcctat
1981 ttttcaatgg gaagactcag gagtctgcca cttgtcaaag aaggtgctga tcctaagaat
2041 ttttcattct cagaattcgg tgtgctgcca acttgatgtt ccacctgcca caaaccacca
2101 ggactgaaag aagaaaacag tacagaaggc aaagtttaca gatgttttta attctagtat
2161 tttatctgga acaacttgta gcagctatat atttcccctt ggtcccaagc ctgatacttt
2221 agccatcata actcactaac agggagaagt agctagtagc aatgtgcctt gattgattag
2281 ataaagattt ctagtaggca gcaaaagacc aaatctcagt tgtttgcttc ttgccatcac
2341 tggtccaggt cttcagtttc cgaatctctt tcccttcccc tgtggtctat tgtcgctatg
2401 tgacttgcgc ttaatccaat attttgcctt ttttctatat caaaaaacct ttacagttag
2461 cagggatgtt ccttaccaag gatttttagc cccaaatctc tcatattcgc tagtgtttaa
2521 aaggctaaga atagtggggc ccagccgatg tggtaggtga taaagaggca tcttttctag
2581 agacacattg aaccagatga ggatccgaaa cggcagcctt tacgttcatc acctgctaga
2641 acctctcgta gtccatcacc atttcttggc attggaattc tactggaaaa aaatacaaaa
2701 agcaaaacaa aaccctcagc actgttacaa gaggccattt aagtatcttg tgcttcttca
2761 cttacccatt agccaggttc tcattaggtt ttgcttgggc ctccctggca ctgaacctta
2821 ggctttgtat gacagtgaag cagcactgtg agtggttcaa gcacactgga atataaaaca
2881 gtcatggcct gagatgcagg tgatgccatt acagaaccaa atcgtggcac gtattgctgt
2941 gtctcctctc agagtgacag tcataaatac tgtcaaacaa taaagggaga atggtgctgt
3001 ttaaagtcac atccctgtaa attgcagaat tcaaaagtga ttatctcttt gatctacttg
3061 cctcatttcc ctatcttctc ccccacggta tcctaaactt tagacttccc actgttctga
3121 aaggagacat tgctctatgt ctgccttcga ccacagcaag ccatcatcct ccattgctcc
3181 cggggactca agaggaatct gtttctctgc tgtcaacttc ccatctggct cagcataggg
3241 tcactttgcc attatgcaaa tggagataaa agcaattctg actgtccagg agctaatctg
3301 accgttctat tgtgtggatg accacataag aaggcaattt tagtgtatta atcatagatt
3361 attataaact ataaacttaa gggcaaggag tttattacaa tgtatcttta ttaaaacaaa
3421 agggtgtata gtgttcacaa actgtgaaaa tagtgtaaga actgtacatt gtgagctctg
3481 gttatttttc tcttgtacca tagaaaaatg tataaaaatt atcaaaaagc taatgtgcag
3541 ggatattgcc ttatttgtct gtaaaaaatg gagctcagta acataactgc ttcttggagc
3601 tttggaatat tttatcctgt attcttgttt gaattcctcc tctatttaag atatatacat
3661 ggaatcgaag tgtttatgta atagttctat ccttttgcct gcaggtcagt tgtaataaat
3721 ctaggatgtg atgatgactt tgtaatttga ttttctgaaa tcagaccctg agaggggaaa
3781 atcttaaagt aaattacatt aaattatctg tgcatttcac accaggaaaa aaaaaaaaaa
3841 a
//