LOCUS BC057857 1654 bp mRNA linear ROD 08-OCT-2003
DEFINITION Mus musculus epoxide hydrolase 1, microsomal, mRNA (cDNA clone
MGC:68180 IMAGE:5067895), complete cds.
ACCESSION BC057857
VERSION BC057857.1
KEYWORDS MGC.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 1654)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1654)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (08-SEP-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Gilbert Smith, Ph.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 123 Row: g Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 6753761.
FEATURES Location/Qualifiers
source 1..1654
/organism="Mus musculus"
/mol_type="mRNA"
/strain="Czech II"
/db_xref="taxon:10090"
/clone="MGC:68180 IMAGE:5067895"
/tissue_type="Mammary tumor metastatized to lung. Tumor
arose spontaneously from a senescent normal mammary
(clonal) outgrowth infected with the virus MMTV."
/clone_lib="NCI_CGAP_Lu29"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1654
/gene="Ephx1"
/db_xref="GeneID:13849"
/db_xref="MGI:MGI:95405"
CDS 70..1395
/gene="Ephx1"
/codon_start=1
/product="Ephx1 protein"
/protein_id="AAH57857.1"
/db_xref="GeneID:13849"
/db_xref="MGI:MGI:95405"
/translation="MWLELILASVLGFVIYWFVSRDKEETLPLEDGWWGPGSKPSAKE
DESIRPFKVETSDEEIKDLHQRIDRFRASPPLEGSRFHYGFNSSYLKKVVSFWRNEFD
WRKQVEILNQYPHFKTKIEGLDIHFIHVKPPQLPSGRTPKPLLMVHGWPGSFYEFYKI
IPLLTDPKTHGLSDEHVFEVIFPSIPGYGFSEASSKKGLNSVATARIFYKLMSRLGFQ
KFYIQGGDWGSLICTNIAQMVPKNIYSLTPLLGQRFGRFLGYTEKDLELLYPFKEKVF
YNIMRESGYLHIQATKPDTVGCALNDSPVGLAAYILEKFSTWTKSEYCELEDGGLERK
FSLEDLLTNIMIYWTTGTIVSSQRFYKENLGQGVMVHRHEGMKVFVPTGYSAFPSEIL
HAPEKWVKVKYPKLISYSYMERGGHFAAFEEPKLLAQDIRKFVSLAELQ"
misc_feature 601..1197
/gene="Ephx1"
/note="abhydrolase; Region: alpha/beta hydrolase fold.
This catalytic domain is found in a very wide range of
enzymes"
/db_xref="CDD:pfam00561"
BASE COUNT 426 a 444 c 409 g 375 t
ORIGIN
1 tttgagagtg gaggaactgc acaccagccg ccgcgggagt aggaacccga gagcgaccct
61 gacagagtca tgtggctgga actcatcctg gcttctgtgc tgggctttgt catctactgg
121 tttgtctccc gggacaagga ggagacctta ccacttgaag atgggtggtg gggcccaggg
181 tcaaagccat cagccaaaga agatgagagc atccggccct tcaaggtgga aacatcagat
241 gaggagatca aggacttgca ccagaggata gataggttcc gggcatcccc acctttggag
301 ggcagtcgct tccactatgg cttcaactcc agctacctga agaaagtggt gtccttctgg
361 aggaatgagt ttgactggag gaagcaggtg gagatcctca accaataccc acactttaag
421 accaagattg aagggctgga catccacttc atccacgtga aacctcccca gctgccctca
481 ggccgcactc caaagccctt gctgatggtg cacggctggc ctggctcctt ctatgagttc
541 tacaagatta tcccactgct gacagacccc aagacccacg gcctgagtga cgagcacgtg
601 tttgaagtca tctttccctc aattcctggc tatggcttct cagaggcatc cagcaagaaa
661 ggtttaaatt cggtggccac tgcgaggatc ttctacaagc tgatgtcacg gctgggcttc
721 cagaagttct acattcaagg cggcgactgg gggtctctca tctgcaccaa catagcccag
781 atggtgccca aaaacattta ttccctgacc cctctcctgg gccaacgttt tgggagattt
841 cttggctaca cagagaagga tctggagctc ttgtacccat tcaaggaaaa ggttttctac
901 aacatcatga gggagagtgg ctacttacac atccaggcca ccaagccgga cactgtgggc
961 tgtgctctga atgactctcc tgtgggcctg gctgcctaca tcttagagaa gttctccacc
1021 tggaccaagt cagaatactg tgaactggag gatggaggcc tggagaggaa gttctccctg
1081 gaagatctgc tgactaacat catgatctac tggacgacag gaaccattgt ctcctcccag
1141 cgcttctaca aggaaaactt gggccagggt gtcatggtcc atagacatga ggggatgaag
1201 gtctttgtgc ccactggcta ttcagccttc ccttctgaga tcctgcatgc cccagaaaag
1261 tgggtgaagg tcaagtaccc caaactcatc tcctattcct acatggaacg tgggggccac
1321 tttgctgcct tcgaagagcc caagcttctg gcccaggaca tccgcaagtt cgtgtccctg
1381 gctgagctgc agtgatgacg ctacacacca accatggctt tagcaacagc cctggttcct
1441 tcccagtcat acttatggaa gatgtcccct tccagaggaa taagtttgtt ccctgaccac
1501 actgggggac ccagacttca accccacaga gtcctctctt accaccccca tatgcttcac
1561 cccactgcat agctgtgtta agctacatgg ctttaatgat aaatgggttt atttctaaaa
1621 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
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