LOCUS BC057805 1767 bp mRNA linear HUM 28-JUL-2005
DEFINITION Homo sapiens eukaryotic translation initiation factor 2-alpha
kinase 2, mRNA (cDNA clone IMAGE:5278139), partial cds.
ACCESSION BC057805
VERSION BC057805.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1767)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1767)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-SEP-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 133 Row: n Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4506102.
FEATURES Location/Qualifiers
source 1..1767
/db_xref="H-InvDB:HIT000259742"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5278139"
/tissue_type="Brain, hypothalamus"
/clone_lib="NIH_MGC_96"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..>1767
/gene="EIF2AK2"
/gene_synonym="EIF2AK1"
/gene_synonym="PKR"
/db_xref="GeneID:5610"
/db_xref="MIM:176871"
CDS 229..>1767
/gene="EIF2AK2"
/gene_synonym="EIF2AK1"
/gene_synonym="PKR"
/codon_start=1
/product="EIF2AK2 protein"
/protein_id="AAH57805.1"
/db_xref="GeneID:5610"
/db_xref="MIM:176871"
/translation="MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQ
VIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYI
GLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKL
AYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSN
SDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSG
GFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDP
ETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLAL
ELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKG
TLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFD
KKKKK"
BASE COUNT 602 a 304 c 393 g 468 t
ORIGIN
1 gcgggtctcc ggccggcggc ggcggcggcg gcggcggcgg cgcagtttct ggagcaaatt
61 cagtttgcct tcctggattt gtaaattgta atgacctcaa aactttagca gttcttccat
121 ctgactcagg tttgcttctc tggcggtctt cagaatcaac atccacactt ccgtgattat
181 ctgcgtgcat tttggacaaa gcttccaacc aggatacggg aagaagaaat ggctggtgat
241 ctttcagcag gtttcttcat ggaggaactt aatacatacc gtcagaagca gggagtagta
301 cttaaatatc aagaactgcc taattcagga cctccacatg ataggaggtt tacatttcaa
361 gttataatag atggaagaga atttccagaa ggtgaaggta gatcaaagaa ggaagcaaaa
421 aatgccgcag ccaaattagc tgttgagata cttaataagg aaaagaaggc agttagtcct
481 ttattattga caacaacgaa ttcttcagaa ggattatcca tggggaatta cataggcctt
541 atcaatagaa ttgcccagaa gaaaagacta actgtaaatt atgaacagtg tgcatcgggg
601 gtgcatgggc cagaaggatt tcattataaa tgcaaaatgg gacagaaaga atatagtatt
661 ggtacaggtt ctactaaaca ggaagcaaaa caattggccg ctaaacttgc atatcttcag
721 atattatcag aagaaacctc agtgaaatct gactacctgt cctctggttc ttttgctact
781 acgtgtgagt cccaaagcaa ctctttagtg accagcacac tcgcttctga atcatcatct
841 gaaggtgact tctcagcaga tacatcagag ataaattcta acagtgacag tttaaacagt
901 tcttcgttgc ttatgaatgg tctcagaaat aatcaaagga aggcaaaaag atctttggca
961 cccagatttg accttcctga catgaaagaa acaaagtata ctgtggacaa gaggtttggc
1021 atggatttta aagaaataga attaattggc tcaggtggat ttggccaagt tttcaaagca
1081 aaacacagaa ttgacggaaa gacttacgtt attaaacgtg ttaaatataa taacgagaag
1141 gcggagcgtg aagtaaaagc attggcaaaa cttgatcatg taaatattgt tcactacaat
1201 ggctgttggg atggatttga ttatgatcct gagaccagtg atgattctct tgagagcagt
1261 gattatgatc ctgagaacag caaaaatagt tcaaggtcaa agactaagtg ccttttcatc
1321 caaatggaat tctgtgataa agggaccttg gaacaatgga ttgaaaaaag aagaggcgag
1381 aaactagaca aagttttggc tttggaactc tttgaacaaa taacaaaagg ggtggattat
1441 atacattcaa aaaaattaat tcatagagat cttaagccaa gtaatatatt cttagtagat
1501 acaaaacaag taaagattgg agactttgga cttgtaacat ctctgaaaaa tgatggaaag
1561 cgaacaagga gtaagggaac tttgcgatac atgagcccag aacagatttc ttcgcaagac
1621 tatggaaagg aagtggacct ctacgctttg gggctaattc ttgctgaact tcttcatgta
1681 tgtgacactg cttttgaaac atcaaagttt ttcacagacc tacgggatgg catcatctca
1741 gatatatttg ataaaaaaaa aaaaaaa
//