LOCUS BC056900 5114 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens nischarin, mRNA (cDNA clone MGC:65065 IMAGE:6143316),
complete cds.
ACCESSION BC056900
VERSION BC056900.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5114)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5114)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-AUG-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 119 Row: l Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 66472381.
FEATURES Location/Qualifiers
source 1..5114
/db_xref="H-InvDB:HIT000259625"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:65065 IMAGE:6143316"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..5114
/gene="NISCH"
/gene_synonym="I-1"
/gene_synonym="IRAS"
/gene_synonym="KIAA0975"
/db_xref="GeneID:11188"
/db_xref="HGNC:HGNC:18006"
CDS 5..4519
/gene="NISCH"
/gene_synonym="I-1"
/gene_synonym="IRAS"
/gene_synonym="KIAA0975"
/codon_start=1
/product="nischarin"
/protein_id="AAH56900.1"
/db_xref="GeneID:11188"
/db_xref="HGNC:HGNC:18006"
/translation="MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEW
TVKHRYSDFHDLHEKLVAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAA
FPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQ
LQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKS
LHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLE
GPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNL
VHLDLSYNKLSSLEGLHTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQM
EEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTV
EVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSN
QGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGG
ASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEA
ANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEE
AEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFE
IPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAA
NLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCF
PVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTP
QHLNVIKADFNPIPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVL
ELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADG
QPAERRASNDQRPQEVPAEALAPAPVEVPAPAPAAASASGPAKTPAPAEASTSALVPE
ETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELF
HSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQ
DFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDS
YLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYP
SEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSS
EIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDD
VQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALC
GRELPVELTG"
BASE COUNT 1053 a 1587 c 1459 g 1015 t
ORIGIN
1 gaacatggcg accgcgcgca ccttcgggcc cgagcgggaa gccgagccgg ccaaggaagc
61 gcgcgtcgtg ggctcggagc ttgtggacac ttatacggtt tacatcatcc aggtcactga
121 tggcagccat gagtggacag taaagcaccg ctacagcgac ttccatgacc tgcatgaaaa
181 gctcgttgca gagagaaaga ttgataaaaa cctgcttccg cccaaaaaga taattgggaa
241 aaactcaaga agcttggtgg agaagaggga gaaggatctg gaggtctacc tccagaagct
301 cctggctgcc ttccctggcg tgacccccag agtactggcc cacttcttgc attttcactt
361 ctatgagata aatggcatca ccgcggcact ggctgaagag ctctttgaga aaggagaaca
421 gctcctgggg gccggcgagg tctttgccat tggacccctg cagctgtatg ccgtcacgga
481 gcagctgcag cagggaaagc ccacgtgcgc cagtggggat gccaagaccg acctcgggca
541 catcctggac ttcacctgtc gccttaagta ccttaaggtt tctggcacag aaggaccttt
601 tgggaccagc aacattcagg agcagctcct gccgttcgac ctatcaatat tcaagtccct
661 gcatcaggtg gagataagtc actgtgatgc taagcacatc agagggctgg tcgcatcgaa
721 gcccacctta gccacgctga gtgtccgctt ctcagcaacc tcgatgaagg aagtccttgt
781 tcctgaagcc tcagaatttg atgagtggga gcctgaaggc acaaccctag aaggccctgt
841 gactgccgtc atccccactt ggcaggcatt gaccacgctt gacctgagcc acaacagcat
901 ctccgagatc gacgagtctg tgaaactgat cccaaagatt gagttcctgg acctgagtca
961 caatggattg ctggttgtgg acaatctgca gcacctgtat aaccttgtgc atctggacct
1021 gtcctacaac aagctctcct ccttggaagg gcttcacacc aagctgggga acatcaagac
1081 cttaaacctg gcaggcaacc tcctagagag tctgagtggc ctgcacaagc tctactcact
1141 ggtcaacctg gatctccggg acaacaggat cgaacagatg gaggaggtcc ggagcatagg
1201 cagcctcccg tgtctggagc acgtgtctct gctgaacaac cctctgagca tcatccccga
1261 ctaccggacc aaggtgctgg ctcagttcgg agagagggcc tcagaggtct gtctggatga
1321 cacagtgacc acagagaagg agctggacac tgtggaagtg ctgaaagcaa ttcagaaagc
1381 caaggaggtc aagtccaaac tgagcaaccc agagaagaag ggtggtgaag actcccggct
1441 ctcagctgcc ccctgcatca gacccagcag ctcccctccc actgtggctc ccgcatctgc
1501 ctccctgccc cagcccatcc tctctaacca aggaatcatg ttcgttcagg aggaggccct
1561 ggccagcagc ctctcgtcca ctgacagtct gactcccgag caccagccca ttgcccaggg
1621 atgttctgat tccttggagt ccatccctgc gggacaggca gcttccgatg atttaaggga
1681 cgtgccagga gctgttggtg gtgcaagccc agaacatgcc gagccggagg tccaggtggt
1741 gccggggtct ggccagatca tcttcctgcc cttcacctgc attggctaca cggccaccaa
1801 tcaggacttc atccagcgcc tgagcacact gatccggcag gccatcgagc ggcagctgcc
1861 tgcctggatc gaggctgcca accagcggga ggagggccag ggtgaacagg gcgaggagga
1921 ggatgaggag gaggaagaag aggaggacgt ggctgagaac cgctactttg aaatggggcc
1981 cccagacgtg gaggaggagg agggaggagg ccagggggag gaagaggagg aggaagagga
2041 ggatgaagag gccgaggagg agcgcctggc tctggaatgg gccctgggcg cggacgagga
2101 cttcctgctg gagcacatcc gcatcctcaa ggtgctgtgg tgcttcctga tccatgtgca
2161 gggcagtatc cgccagttcg ccgcctgcct tgtgctcacc gacttcggca tcgcagtctt
2221 cgagatcccg caccaggagt ctcggggcag cagccagcac atcctctcct ccctgcgctt
2281 tgtcttttgc ttcccgcatg gcgacctcac cgagtttggc ttcctcatgc cggagctgtg
2341 tctggtgctc aaggtacggc acagtgagaa cacgctcttc attatctcgg acgccgccaa
2401 cctgcacgag ttccacgcgg acctgcgctc atgctttgca ccccagcaca tggccatgct
2461 gtgtagcccc atcctctacg gcagccacac cagcctgcag gagttcctgc gccagctgct
2521 caccttctac aaggtggctg gcggctgcca ggagcgcagc cagggctgct tccccgtcta
2581 cctggtctac agtgacaagc gcatggtgca gacggccgcc ggggactact caggcaacat
2641 cgagtgggcc agctgcacac tctgttcagc cgtgcggcgc tcctgctgcg cgccctctga
2701 ggccgtcaag tccgccgcca tcccctactg gctgttgctc acgccccagc acctcaacgt
2761 catcaaggcc gacttcaacc ccatccccaa ccgtggcacc cacaactgtc gcaaccgcaa
2821 cagcttcaag ctcagccgtg tgccgctctc caccgtgctg ctggacccca cacgcagctg
2881 tacccagcct cggggcgcct ttgctgatgg ccacgtgcta gagctgctcg tggggtaccg
2941 ctttgtcact gccatcttcg tgctgcccca cgagaagttc cacttcctgc gcgtctacaa
3001 ccagctgcgg gcctcgctgc aggacctgaa gactgtggtc atcgccaaga cccccgggac
3061 gggaggcagc ccccagggct cctttgcgga tggccagcct gccgagcgca gggccagcaa
3121 tgaccagcgt ccccaggagg tcccagcaga ggctctggcc ccggccccag tggaagtccc
3181 agctccagcc cctgcagcag cctcagcctc aggcccagcg aagactccgg ccccagcaga
3241 ggcctcaact tcagctttgg tcccagagga gacgccagtg gaagctccag ccccaccccc
3301 agccgaggcc cctgcccagt acccgagtga gcacctcatc caggccacct cggaggagaa
3361 tcagatcccc tcgcacttgc ctgcctgccc gtcgctccgg cacgtcgcca gcctgcgggg
3421 cagcgccatc atcgagctct tccacagcag cattgctgag gttgaaaacg aggagctgag
3481 gcacctcatg tggtcctcgg tggtgttcta ccagacccca gggctggagg tgactgcctg
3541 cgtgctgctc tccaccaagg ctgtgtactt tgtgctccac gacggcctcc gccgctactt
3601 ctcagagcca ctgcaggatt tctggcatca gaaaaacacc gactacaaca acagcccttt
3661 ccacatctcc cagtgcttcg tgctaaagct tagtgacctg cagtcagtca atgtggggct
3721 tttcgaccag catttccggc tgacgggttc caccccgatg caggtggtca cgtgcttgac
3781 gcgggacagc tacctgacgc actgcttcct ccagcacctc atggtcgtgc tgtcctctct
3841 ggaacgcacg ccctcgccgg agcctgttga caaggacttc tactccgagt ttgggaacaa
3901 gaccacaggg aagatggaga actacgagct gatccactct agtcgcgtca agtttaccta
3961 ccccagtgag gaggagattg gggacctgac gttcactgtg gcccaaaaga tggctgagcc
4021 agagaaggcc ccagccctca gcatcctgct gtacgtgcag gccttccagg tgggcatgcc
4081 accccctggg tgctgcaggg gccccctgcg ccccaagaca ctcctgctca ccagctccga
4141 gatcttcctc ctggatgagg actgtgtcca ctacccactg cccgagtttg ccaaagagcc
4201 gccgcagaga gacaggtacc ggctggacga tggccgccgc gtccgggacc tggaccgagt
4261 gctcatgggc taccagacct acccgcaggc cctcaccctc gtcttcgatg acgtgcaagg
4321 tcatgacctc atgggcagtg tcaccctgga ccactttggg gaggtgccag gtggcccggc
4381 tagagccagc cagggccgtg aagtccagtg gcaggtgttt gtccccagtg ctgagagcag
4441 agagaagctc atctcgctgt tggctcgcca gtgggaggcc ctgtgtggcc gtgagctgcc
4501 tgtcgagctc accggctagc ccaggccaca gccagcctgt cgtgtccagc ctgacgccta
4561 ctggggcagg gcagcaggct tttgtgttct ctaaaaatgt tttatcctcc ctttggtacc
4621 ttaatttgac tgtcctcgca gagaatgtga acatgtgtgt gtgttgtgtt aattctttct
4681 catgttggga gtgagaatgc cgggcccctc agggctgtcg gtgtgctgtc agcctcccac
4741 aggtggtaca gccgtgcaca ccagtgtcgt gtctgctgtt gtgggaccgt tgttaacacg
4801 tgacactgtg ggtctgactt tctcttctac acgtcctttc ctgaagtgtc gagtccagtc
4861 ctttgttgct gttgctgttg ctgttgctgt tgctgttggc atcttgctgc taatcctgag
4921 gctggtagca gaatgcacat tggaagctcc caccccatat tgttcttcaa agtggaggtc
4981 tcccctgatc cagacaagtg ggagagcccg tgggggcagg ggacctggag ctgccagcac
5041 caagcgtgat tcctgctgcc tgtattctct attccaataa agcagagttt gacaccgtca
5101 aaaaaaaaaa aaaa
//