LOCUS BC056891 3055 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens caspase recruitment domain family, member 8, mRNA
(cDNA clone MGC:65033 IMAGE:6144772), complete cds.
ACCESSION BC056891
VERSION BC056891.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3055)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3055)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-AUG-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 119 Row: l Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7662403
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..3055
/db_xref="H-InvDB:HIT000259616"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:65033 IMAGE:6144772"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3055
/gene="CARD8"
/gene_synonym="CARDINAL"
/gene_synonym="DACAR"
/gene_synonym="Dakar"
/gene_synonym="NDPP"
/gene_synonym="NDPP1"
/gene_synonym="TUCAN"
/db_xref="GeneID:22900"
/db_xref="HGNC:HGNC:17057"
/db_xref="MIM:609051"
CDS 347..1525
/gene="CARD8"
/gene_synonym="CARDINAL"
/gene_synonym="DACAR"
/gene_synonym="Dakar"
/gene_synonym="NDPP"
/gene_synonym="NDPP1"
/gene_synonym="TUCAN"
/codon_start=1
/product="CARD8 protein"
/protein_id="AAH56891.1"
/db_xref="GeneID:22900"
/db_xref="HGNC:HGNC:17057"
/db_xref="MIM:609051"
/translation="MEKKECPEKSSSSEEELPRRDSGSSRNIDASKLIRLQGSRKLLV
DNSIRELQYTKTGIFFQAEACVTNDTVYRELPCVSETLCDISHFFQEDDETEAEPLLF
RAVPECQLSGGDIPSVSEEQESSEGQDSGDICSEENQIVSSYASKVCFEIEEDYKNRQ
FLGPEGNVDVELIDKSTNRYSVWFPTAGWYLWSATGLGFLVRDEVTVTIAFGSWSQHL
ALDLQHHEQWLVGGPLFDVTAEPEEAVAEIHLPHSISLQAGEVDVSWFLVAHFKNEGM
VLEHPARVEPFYAVLESPSFSLMGILLRIASGTRLSIPITSNTLIYYHPHPEDIKFHL
YLVPSDALLTKAIDDEEDRFHGVRLQTSPPMEPLNFGSSYIVSNSANLKVMPKWISSL
"
BASE COUNT 865 a 662 c 691 g 837 t
ORIGIN
1 gttgagaacc agagaaaccg acctaagggt attctcccat ttggcccgtc ctcccctaaa
61 gtcaccacct gctgcttttc tggagcgctt accagtgacc aaggggaaca gaacacagag
121 cagcctggca gtgtccaagc aacaagcctc cgctcctcct tcctgcaccc tggggctcct
181 gaaactcaca tgagaaggag ggctgtctga gattcgaggg aaacaagctc tcaggacttc
241 cggtcgccat gatggctgtg ggcggtaaac gcggttagtg caagcatctg ggccatcttc
301 aatggtaaaa aagatacagt aaagacataa ataccacatt tgacaaatgg aaaaaaagga
361 gtgtccagaa aagagtagca gcagtgagga agagctgccg agacgggaca gtggatccag
421 taggaacata gatgcatcca aactcattag actacaagga tcacggaaac tgttggttga
481 caatagcata cgggaactgc aatacacaaa aactggaatt ttttttcagg ctgaggcctg
541 tgtgacaaat gatacggtat acagggagct accctgtgtt tctgagaccc tctgtgacat
601 ctcacatttt ttccaagaag atgatgagac agaggcagag ccattattgt tccgtgctgt
661 tcctgagtgt caactatctg ggggggacat tcccagtgta tcagaagagc aggaatcttc
721 agagggacaa gattcaggag acatttgctc agaagagaat caaatagttt cctcttatgc
781 ttctaaagtc tgttttgaga tcgaagaaga ttataaaaat cgtcagtttc tggggcctga
841 aggaaatgtg gatgttgagt tgattgataa gagcacaaac agatacagcg tttggttccc
901 cactgctggc tggtatctgt ggtcagccac aggcctcggc ttcctggtaa gggatgaggt
961 cacagtgacg attgcgtttg gttcctggag tcagcacctg gccctggacc tgcagcacca
1021 tgaacagtgg ctggtgggcg gccccttgtt tgatgtcact gcagagccag aggaggctgt
1081 cgccgaaatc cacctccccc actccatctc cctccaagca ggtgaggtgg acgtctcctg
1141 gtttctcgtt gcccatttta agaatgaagg gatggtcctg gagcatccag cccgggtgga
1201 gcctttctat gctgtcctgg aaagccccag cttctctctg atgggcatcc tgctgcggat
1261 cgccagtggg actcgcctct ccatccccat cacttccaac acattgatct attatcaccc
1321 ccaccccgaa gatattaagt tccacttgta ccttgtcccc agcgacgcct tgctaacaaa
1381 ggcgatagat gatgaggaag atcgcttcca tggtgtgcgc ctgcagactt cgcccccaat
1441 ggaacccctg aactttggtt ccagttatat tgtgtctaat tctgctaacc tgaaagtaat
1501 gcccaagtgg atctccagct tgtagctgca tcagcccctc ctcctttctc aggtgcagcc
1561 tttgtgaagg agaaccaccg gcaactccaa gccaggatgg gggacctgaa aggggtgctc
1621 gatgatctcc aggacaatga ggttcttact gagaatgaga aggagctggt ggagcaggaa
1681 aagacacggc agagcaagaa tgaggccttg ctgagcatgg tggagaagaa aggggacctg
1741 gccctggacg tgctcttcag aagcattagt gaaagggacc cttacctcgt gtcctatctt
1801 agacagcaga atttgtaaaa tgagtcagtt aggtagtctg gaagagagaa tccagcgttc
1861 tcattggaaa tggataaaca gaaatgtgat cattgatttc agtgttcaag acagaagaag
1921 actgggtaac atctatcaca caggctttca ggacagactt gtaacctggc atgtacctat
1981 tgactgtatc ctcatgcatt ttcctcaaga atgtctgaag aaggtagtaa tattcctttt
2041 aaattttttc caaccattgc ttgatatatc actattttat ccattgacat gattcttgaa
2101 gacccaggat aaaggacatc cggataggtg tgtttatgaa ggatggggcc tggaaaggca
2161 acttttcctg attaatgtga aaaataattc ctatggacac tccgtttgaa gtatcacctt
2221 ctcataacta aaagcagaaa agctaacaaa agcttctcag ctgaggacac tcaaggcata
2281 catgatgaca gtcttttttt tttttgtatg ttaggacttt aacactttat ctatggctac
2341 tgttattaga acaatgtaaa tgtatttgct gaaagagagc acaaaaatgg gagaaaatgc
2401 aaacatgagc agaaaatatt ttcccactgg tgtgtagcct gctacaagga gttgttgggt
2461 taaatgttca tggtcaactc caaggaatac tgagatgaaa tgtggtaaat caactccaca
2521 gaaccaccaa aaagaaaatg agggtaattc agcttattct gagacagaca ttcctggcaa
2581 tgtaccatac aaaaaataag ccaactctga catttggatt ctaccataga ctctgtcatt
2641 ttgtagccat ttcagctgtc ttttgattaa tgttttcgtg gcacacatat ttccatcctt
2701 ttatgtttaa tctgtttaaa acaagttcct agtagacacc atctggttga gtcagttttt
2761 tttatggtgt attttgaacc cattctgata gtctctttta actggaagat ttcaattact
2821 tacgttaatg taattattaa tatgttagga tttatcctca gtcagccagt ttgttatgtc
2881 ttttctattc tactgttatc acatttgtac cacttaaagt ggaatctagg cactttatca
2941 ccatttagat cctattacct tttctcatct aggatatagt tatcttctac ataatctttc
3001 tgtatcttaa aacccatcaa taaattatta tatattttct aaaaaaaaaa aaaaa
//