LOCUS BC054503 4578 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens mitogen-activated protein kinase kinase kinase 5, mRNA
(cDNA clone MGC:60047 IMAGE:6143181), complete cds.
ACCESSION BC054503
VERSION BC054503.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4578)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4578)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-JUL-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC054503.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 110 Row: j Column: 3.
FEATURES Location/Qualifiers
source 1..4578
/db_xref="H-InvDB:HIT000054078"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:60047 IMAGE:6143181"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4578
/gene="MAP3K5"
/gene_synonym="ASK1"
/gene_synonym="MAPKKK5"
/db_xref="GeneID:4217"
/db_xref="HGNC:HGNC:6857"
/db_xref="MIM:602448"
CDS 309..4433
/gene="MAP3K5"
/gene_synonym="ASK1"
/gene_synonym="MAPKKK5"
/codon_start=1
/product="mitogen-activated protein kinase kinase kinase
5"
/protein_id="AAH54503.1"
/db_xref="GeneID:4217"
/db_xref="HGNC:HGNC:6857"
/db_xref="MIM:602448"
/translation="MSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEH
QLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQ
GQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAF
RQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMIT
PHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESI
LNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLV
ETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCL
VGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSF
ELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPA
WYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEP
TKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFL
YVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGD
RVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQY
LGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQ
IVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPR
GYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAF
ILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVL
VEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSA
PPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHIT
TLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHN
IKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSN
QTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRK
EKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVN
GADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT"
BASE COUNT 1303 a 1019 c 1160 g 1096 t
ORIGIN
1 cagccggcca agaaaaggaa gctccgtccc ttcccgctca cccggcttcc ccaccccttg
61 tactctaaac tctgcagagg gcgagcggcg cggccacgga ggcgccgagg aggagcgagc
121 cgccgccggg cagcggcgtg ccctcggggg agagggcgcc ggagaggagg cggcggcgcg
181 gcggcgaggg cgcggcgcgc gatggcagct gcttagcccg gcgggcgcgg agcagccccg
241 agctgtggct ggccaggcgg tgcggctggg cgggggacgc cgccgccgtt gctgcccggc
301 ccggagagat gagcacggag gcggacgagg gcatcacttt ctctgtgcca cccttcgccc
361 cctcgggctt ctgcaccatc cccgagggcg gcatctgcag gaggggagga gcggcggcgg
421 tgggcgaggg cgaggagcac cagctgccac cgccgccgcc gggcagtttc tggaacgtgg
481 agagcgccgc tgcccctggc atcggttgtc cggcggccac ctcctcgagc agtgccaccc
541 gaggccgggg cagctctgtt ggcgggggca gccgacggac cacggtggca tatgtgatca
601 acgaagcgag ccaagggcaa ctggtggtgg ccgagagcga ggccctgcag agcttgcggg
661 aggcgtgcga gacagtgggc gccaccctgg aaaccctgca ttttgggaaa ctcgactttg
721 gagaaaccac cgtgctggac cgcttttaca atgcagatat tgcggtggtg gagatgagcg
781 atgccttccg gcagccgtcc ttgttttacc accttggggt gagagaaagt ttcagcatgg
841 ccaacaacat catcctctac tgtgatacta actcggactc tctgcagtca ctgaaggaaa
901 tcatttgcca gaagaatact atgtgcactg ggaactacac ctttgttcct tacatgataa
961 ctccacataa caaagtctac tgctgtgaca gcagcttcat gaaggggttg acagagctca
1021 tgcaaccgaa cttcgagctg cttcttggac ccatctgctt acctcttgtg gatcgtttta
1081 ttcaactttt gaaggtggca caagcaagtt ctagccagta cttccgggaa tctatactca
1141 atgacatcag gaaagctcgt aatttataca ctggtaaaga attggcagct gagttggcaa
1201 gaattcggca gcgagtagat aatatcgaag tcttgacagc agatattgtc ataaatctgt
1261 tactttccta cagagatatc caggactatg attctattgt gaagctggta gagactttag
1321 aaaaactgcc aacctttgat ttggcctccc atcaccatgt gaagtttcat tatgcatttg
1381 cactgaatag gagaaatctc cctggtgaca gagcaaaagc tcttgatatt atgattccca
1441 tggtgcaaag cgaaggacaa gttgcttcag atatgtattg cctagttggt cgaatctaca
1501 aagatatgtt tttggactct aatttcacgg acactgaaag cagagaccat ggagcttctt
1561 ggttcaaaaa ggcatttgaa tctgagccaa cactacagtc aggaattaat tatgcggtcc
1621 tcctcctggc agctggacac cagtttgaat cttcctttga gctccggaaa gttggggtga
1681 agctaagtag tcttcttggt aaaaagggaa acttggaaaa actccagagc tactgggaag
1741 ttggattttt tctgggggcc agcgtcctag ccaatgacca catgagagtc attcaagcat
1801 ctgaaaagct ttttaaactg aagacaccag catggtacct caagtctatt gtagagacaa
1861 ttttaatata taagcatttt gtgaaactga ccacagaaca gcctgtggcc aagcaagaac
1921 ttgtggactt ttggatggat ttcctggtcg aggccacaaa gacagatgtt actgtggtta
1981 ggtttccagt attaatatta gaaccaacca aaatctatca accttcttat ttgtctatca
2041 acaatgaagt tgaggaaaag acaatctcta tttggcacgt gcttcctgat gacaagaaag
2101 gtatacatga gtggaatttt agtgcctctt ctgtcagggg agtgagtatt tctaaatttg
2161 aagaaagatg ctgctttctt tatgtgcttc acaattctga tgatttccaa atctatttct
2221 gtacagaact tcattgtaaa aagttttttg agatggtgaa caccattacc gaagagaagg
2281 ggagaagcac agaggaagga gactgtgaaa gtgacttgct ggagtatgac tatgaatatg
2341 atgaaaatgg tgacagagtc gttttaggaa aaggcactta tgggatagtc tacgcaggtc
2401 gggacttgag caaccaagtc agaattgcta ttaaggaaat cccagagaga gacagcagat
2461 actctcagcc cctgcatgaa gaaatagcat tgcataaaca cctgaagcac aaaaatattg
2521 tccagtatct gggctctttc agtgagaatg gtttcattaa aatcttcatg gagcaggtcc
2581 ctggaggaag tctttctgct ctccttcgtt ccaaatgggg tccattaaaa gacaatgagc
2641 aaacaattgg cttttataca aagcaaatac tggaaggatt aaaatatctc catgacaatc
2701 agatagttca ccgggacata aagggtgaca atgtgttgat taatacctac agtggtgttc
2761 tcaagatctc tgacttcgga acatcaaaga ggcttgctgg cataaacccc tgtactgaaa
2821 cttttactgg taccctccag tatatggcac cagaaataat agataaagga ccaagaggct
2881 acggaaaagc agcagacatc tggtctctgg gctgtacaat cattgaaatg gccacaggaa
2941 aacccccatt ttatgaactg ggagaaccac aagcagctat gttcaaggtg ggaatgttta
3001 aagtccaccc tgagatccca gagtccatgt ctgcagaggc caaggcattc atactgaaat
3061 gttttgaacc agatcctgac aagagagcct gtgctaacga cttgcttgtt gatgagtttt
3121 taaaagtttc aagcaaaaag aaaaagacac aacctaagct ttcagctctt tcagctggat
3181 caaatgaata tctcaggagt atatccttgc cggtacctgt gctggtggag gacaccagca
3241 gcagcagtga gtacggctca gtttcacccg acacggagtt gaaagtggac cccttctctt
3301 tcaaaacaag agccaagtcc tgcggagaaa gagatgtcaa gggaattcgg acactctttt
3361 tgggcattcc agatgagaat tttgaagatc acagtgctcc tccttcccct gaagaaaaag
3421 attctggatt cttcatgctg aggaaggaca gtgagaggcg agctaccctt cacaggatcc
3481 tgacggaaga ccaagacaaa attgtgagaa acctaatgga atctttagct cagggggctg
3541 aagaaccgaa actaaaatgg gaacacatca caaccctcat tgcaagcctc agagaatttg
3601 tgagatccac tgaccgaaaa atcatagcca ccacactgtc aaagctgaaa ctggagctgg
3661 acttcgacag ccatggcatt agccaagtcc aggtggtact ctttggtttt caagatgctg
3721 tcaataaagt tcttcggaat cataacatca agccgcactg gatgtttgcc ttagacagta
3781 tcattcggaa ggcggtacag acagccatta ccatcctggt tccagaacta aggccacatt
3841 tcagccttgc atctgagagt gatactgctg atcaagaaga cttggatgta gaagatgacc
3901 atgaggaaca gccttcaaat caaactgtcc gaagacctca ggctgtcatt gaagatgctg
3961 tggctacctc aggcgtgagc acgctcagtt ctactgtgtc tcatgattcc cagagtgctc
4021 accggtcact gaatgtacag cttggaagga tgaaaataga aaccaataga ttactggaag
4081 aattggttcg gaaagagaaa gaattacaag cactccttca tcgagctatt gaagaaaaag
4141 accaagaaat taaacacctg aagcttaagt cccaacccat agaaattcct gaattgcctg
4201 tatttcatct aaattcttct ggcacaaata ctgaagattc tgaacttacc gactggctga
4261 gagtgaatgg agctgatgaa gacactataa gccggttttt ggctgaagat tatacactat
4321 tggatgttct ctactatgtt acacgtgatg acttaaaatg cttgagacta aggggaggga
4381 tgctgtgcac actgtggaag gctatcattg actttcgaaa caaacagact tgactgttgc
4441 tcaatctaat cttcgatgga aattctaaaa attaatacag agctgatctt cttgggggtg
4501 ggaaaatcga agggagagga gaaaggcgct gcactttaaa tccagtattt gtttactcat
4561 gttaaaaaaa aaaaaaaa
//