LOCUS BC054494 5143 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens nischarin, mRNA (cDNA clone MGC:54210 IMAGE:6195532),
complete cds.
ACCESSION BC054494
VERSION BC054494.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5143)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5143)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-JUL-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 98 Row: m Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 66472381.
FEATURES Location/Qualifiers
source 1..5143
/db_xref="H-InvDB:HIT000054071"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:54210 IMAGE:6195532"
/tissue_type="Peripheral Nervous System, sympathetic
trunk"
/clone_lib="Lupski_sympathetic_trunk"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..5143
/gene="NISCH"
/gene_synonym="I-1"
/gene_synonym="IRAS"
/gene_synonym="KIAA0975"
/db_xref="GeneID:11188"
/db_xref="HGNC:HGNC:18006"
CDS 33..4547
/gene="NISCH"
/gene_synonym="I-1"
/gene_synonym="IRAS"
/gene_synonym="KIAA0975"
/codon_start=1
/product="nischarin"
/protein_id="AAH54494.1"
/db_xref="GeneID:11188"
/db_xref="HGNC:HGNC:18006"
/translation="MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEW
TVKHRYSDFHDLHEKLVAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAA
FPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQ
LQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKS
LHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLE
GPVTAVIPTWQALTTLDLSHNSISEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNL
VHLDLSYNKLSSLEGLHTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQM
EEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTV
EVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSN
QGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGG
ASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEA
ANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEE
AEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFE
IPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAA
NLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCF
PVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTP
QHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVL
ELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADG
QPAERRASNDQRPQEVPAEALAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPE
ETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELF
HSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQ
DFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDS
YLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYP
SEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSS
EIFFLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDD
VQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALC
GRELPVELTG"
BASE COUNT 1056 a 1596 c 1475 g 1016 t
ORIGIN
1 cgccgggcgg cggtggcggc ggagacccga acatggcgac cgcgcgcacc ttcgggcccg
61 agcgggaagc cgagccggcc aaggaagcgc gcgtcgtggg ctcggagctt gtggacactt
121 atacggttta catcatccag gtcactgatg gcagccatga gtggacagta aagcaccgct
181 acagcgactt ccatgacctg catgaaaagc tcgttgcaga gagaaagatt gataaaaacc
241 tgcttccgcc caaaaagata attgggaaaa actcaagaag cttggtggag aagagggaga
301 aggatctgga ggtctacctc cagaagctcc tggctgcctt ccctggcgtg acccccagag
361 tactggccca cttcttgcat tttcacttct atgagataaa tggcatcacc gcggcactgg
421 ctgaagagct ctttgagaaa ggagaacagc tcctgggggc cggcgaggtc tttgccattg
481 gacccctgca gctgtatgcc gtcacggagc agctgcagca gggaaagccc acgtgcgcca
541 gtggggatgc caagaccgac ctcgggcaca tcctggactt cacctgtcgc cttaagtacc
601 ttaaggtttc tggcacagaa ggaccttttg ggaccagcaa cattcaggag cagctcctgc
661 cgttcgacct atcaatattc aagtccctgc atcaggtgga gataagtcac tgtgatgcta
721 agcacatcag agggctggtc gcatcgaagc ccaccttagc cacgctgagt gtccgcttct
781 cagcaacctc gatgaaggaa gtccttgttc ctgaagcctc agaatttgat gagtgggagc
841 ctgaaggcac aaccctagaa ggccctgtga ctgccgtcat ccccacttgg caggcattga
901 ccacgcttga cctgagccac aacagcatct ccgagatcga cgagtctgtg aaactgatcc
961 caaagattga gttcctggac ctgagtcaca atggattgct ggttgtggac aatctgcagc
1021 acctgtataa ccttgtgcat ctggacctgt cctacaacaa gctctcctcc ttggaagggc
1081 ttcacaccaa gctggggaac atcaagacct taaacctggc aggcaacctc ctagagagtc
1141 tgagtggcct gcacaagctc tactcactgg tcaacctgga tctccgggac aacaggatcg
1201 aacagatgga ggaggtccgg agcataggca gcctcccgtg tctggagcac gtgtctctgc
1261 tgaacaaccc tctgagcatc atccccgact accggaccaa ggtgctggct cagttcggag
1321 agagggcctc agaggtctgt ctggatgaca cagtgaccac agagaaggag ctggacactg
1381 tggaagtgct gaaagcaatt cagaaagcca aggaggtcaa gtccaaactg agcaacccag
1441 agaagaaggg tggtgaagac tcccggctct cagctgcccc ctgcatcaga cccagcagct
1501 cccctcccac tgtggctccc gcatctgcct ccctgcccca gcccatcctc tctaaccaag
1561 gaatcatgtt cgttcaggag gaggccctgg ccagcagcct ctcgtccact gacagtctga
1621 ctcccgagca ccagcccatt gcccagggat gttctgattc cttggagtcc atccctgcgg
1681 gacaggcagc ttccgatgat ttaagggacg tgccaggagc tgttggtggt gcaagcccag
1741 aacatgccga gccggaggtc caggtggtgc cggggtctgg ccagatcatc ttcctgccct
1801 tcacctgcat tggctacacg gccaccaatc aggacttcat ccagcgcctg agcacactga
1861 tccggcaggc catcgagcgg cagctgcctg cctggatcga ggctgccaac cagcgggagg
1921 agggccaggg tgaacagggc gaggaggagg atgaggagga ggaagaagag gaggacgtgg
1981 ctgagaaccg ctactttgaa atggggcccc cagacgtgga ggaggaggag ggaggaggcc
2041 agggggagga agaggaggag gaagaggagg atgaagaggc cgaggaggag cgcctggctc
2101 tggaatgggc cctgggcgcg gacgaggact tcctgctgga gcacatccgc atcctcaagg
2161 tgctgtggtg cttcctgatc catgtgcagg gcagtatccg ccagttcgcc gcctgccttg
2221 tgctcaccga cttcggcatc gcagtcttcg agatcccgca ccaggagtct cggggcagca
2281 gccagcacat cctctcctcc ctgcgctttg tcttttgctt cccgcatggc gacctcaccg
2341 agtttggctt cctcatgccg gagctgtgtc tggtgctcaa ggtacggcac agtgagaaca
2401 cgctcttcat tatctcggac gccgccaacc tgcacgagtt ccacgcggac ctgcgctcat
2461 gctttgcacc ccagcacatg gccatgctgt gtagccccat cctctacggc agccacacca
2521 gcctgcagga gttcctgcgc cagctgctca ccttctacaa ggtggctggc ggctgccagg
2581 agcgcagcca gggctgcttc cccgtctacc tggtctacag tgacaagcgc atggtgcaga
2641 cggccgccgg ggactactca ggcaacatcg agtgggccag ctgcacactc tgttcagccg
2701 tgcggcgctc ctgctgcgcg ccctctgagg ccgtcaagtc cgccgccatc ccctactggc
2761 tgttgctcac gccccagcac ctcaacgtca tcaaggccga cttcaacccc atgcccaacc
2821 gtggcaccca caactgtcgc aaccgcaaca gcttcaagct cagccgtgtg ccgctctcca
2881 ccgtgctgct ggaccccaca cgcagctgta cccagcctcg gggcgccttt gctgatggcc
2941 acgtgctaga gctgctcgtg gggtaccgct ttgtcactgc catcttcgtg ctgccccacg
3001 agaagttcca cttcctgcgc gtctacaacc agctgcgggc ctcgctgcag gacctgaaga
3061 ctgtggtcat cgccaagacc cccgggacgg gaggcagccc ccagggctcc tttgcggatg
3121 gccagcctgc cgagcgcagg gccagcaatg accagcgtcc ccaggaggtc ccagcagagg
3181 ctctggcccc ggccccagcg gaagtcccag ctccagcccc tgcagcagcc tcagcctcag
3241 gcccagcgaa gactccggcc ccagcagagg cctcaacttc agctttggtc ccagaggaga
3301 cgccagtgga agctccagcc ccacccccag ccgaggcccc tgcccagtac ccgagtgagc
3361 acctcatcca ggccacctcg gaggagaatc agatcccctc gcacttgcct gcctgcccgt
3421 cgctccggca cgtcgccagc ctgcggggca gcgccatcat cgagctcttc cacagcagca
3481 ttgctgaggt tgaaaacgag gagctgaggc acctcatgtg gtcctcggtg gtgttctacc
3541 agaccccagg gctggaggtg actgcctgcg tgctgctctc caccaaggct gtgtactttg
3601 tgctccacga cggcctccgc cgctacttct cagagccact gcaggatttc tggcatcaga
3661 aaaacaccga ctacaacaac agccctttcc acatctccca gtgcttcgtg ctaaagctta
3721 gtgacctgca gtcagtcaat gtggggcttt tcgaccagca tttccggctg acgggttcca
3781 ccccgatgca ggtggtcacg tgcttgacgc gggacagcta cctgacgcac tgcttcctcc
3841 agcacctcat ggtcgtgctg tcctctctgg aacgcacgcc ctcgccggag cctgttgaca
3901 aggacttcta ctccgagttt gggaacaaga ccacagggaa gatggagaac tacgagctga
3961 tccactctag tcgcgtcaag tttacctacc ccagtgagga ggagattggg gacctgacgt
4021 tcactgtggc ccaaaagatg gctgagccag agaaggcccc agccctcagc atcctgctgt
4081 acgtgcaggc cttccaggtg ggcatgccac cccctgggtg ctgcaggggc cccctgcgcc
4141 ccaagacact cctgctcacc agctccgaga tcttcttcct ggatgaggac tgtgtccact
4201 acccactgcc cgagtttgcc aaagagccgc cgcagagaga caggtaccgg ctggacgatg
4261 gccgccgcgt ccgggacctg gaccgagtgc tcatgggcta ccagacctac ccgcaggccc
4321 tcaccctcgt cttcgatgac gtgcaaggtc atgacctcat gggcagtgtc accctggacc
4381 actttgggga ggtgccaggt ggcccggcta gagccagcca gggccgtgaa gtccagtggc
4441 aggtgtttgt ccccagtgct gagagcagag agaagctcat ctcgctgttg gctcgccagt
4501 gggaggccct gtgtggccgt gagctgcctg tcgagctcac cggctagccc aggccacagc
4561 cagcctgtcg tgtccagcct gacgcctact ggggcagggc agcaggcttt tgtgttctct
4621 aaaaatgttt tatcctccct ttggtacctt aatttgactg tcctcgcaga gaatgtgaac
4681 atgtgtgtgt gttgtgttaa ttctttctca tgttgggagt gagaatgccg ggcccctcag
4741 ggctgtcggt gtgctgtcag cctcccacag gtggtacagc cgtgcacacc agtgtcgtgt
4801 ctgctgttgt gggaccgttg ttaacacgtg acactgtggg tctgactttc tcttctacac
4861 gtcctttcct gaagtgtcga gtccagtcct ttgttgctgt tgctgttgct gttgctgttg
4921 ctgttggcat cttgctgcta atcctgaggc tggtagcaga atgcacattg gaagctccca
4981 ccccatattg ttcttcaaag tggaggtctc ccctgatcca gacaagtggg agagcccgtg
5041 ggggcagggg acctggagct gccagcacca agcgtgattc ctgctgcctg tattctctat
5101 tccaataaag cagagtttga caccgtcaaa aaaaaaaaaa aaa
//