LOCUS BC053660 3274 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens polyribonucleotide nucleotidyltransferase 1, mRNA
(cDNA clone MGC:61565 IMAGE:6062060), complete cds.
ACCESSION BC053660
VERSION BC053660.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3274)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3274)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: j Column: 5.
FEATURES Location/Qualifiers
source 1..3274
/db_xref="H-InvDB:HIT000053964"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:61565 IMAGE:6062060"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3274
/gene="PNPT1"
/gene_synonym="old-35"
/gene_synonym="OLD35"
/gene_synonym="PNPASE"
/db_xref="GeneID:87178"
/db_xref="HGNC:HGNC:23166"
CDS 2..2353
/gene="PNPT1"
/gene_synonym="old-35"
/gene_synonym="OLD35"
/gene_synonym="PNPASE"
/codon_start=1
/product="polyribonucleotide nucleotidyltransferase 1"
/protein_id="AAH53660.1"
/db_xref="GeneID:87178"
/db_xref="HGNC:HGNC:23166"
/translation="MAACRYCCSCLRLRPLSDGPFLLPRRDRALTQLQVRALWSSAGS
RAVAVDLGNRKLEISSGKLARFADGSAVVQSGDTAVMVTAVSKTKPSPSQFMPLVVDY
RQKAAAAGRIPTNYLRREVGTSDKEILTSRIIDRSIRPLFPAGYFYDTQVLCNLLAVD
GVNEPDVLAINGASVALSLSDIPWNGPVGAVRIGIIDGEYVVNPTRKEMSSSTLNLVV
AGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKETGVTKRTPQKL
FTPSPEIVKYTHKLAMERLYAVFTDYEHDKVSRDEAVNKIRLDTEEQLKEKFPEADPY
EIIESFNVVAKEVFRSIVLNEYKRCDGRDLTSLRNVSCEVDMFKTLHGSALFQRGQTQ
VLCTVTFDSLESGIKSDQVITAINGIKDKNFMLHYEFPPYATNEIGKVTGLNRRELGH
GALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAG
VAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGI
PIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPG
GYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVY
TATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDP
ADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ"
BASE COUNT 1059 a 588 c 686 g 941 t
ORIGIN
1 catggcggcc tgcaggtact gctgctcgtg cctccggctc cggcccctga gcgatggtcc
61 tttccttctg ccacggcggg atcgggcact cacccagttg caagtgcgag cactatggag
121 tagcgcaggg tctcgagctg tggccgtgga cttaggcaac aggaaattag aaatatcttc
181 tggaaagctg gccagatttg cagatggctc tgctgtagta cagtcaggtg acactgcagt
241 aatggtcaca gcggtcagta aaacaaaacc ttccccttcc cagtttatgc ctttggtggt
301 tgactacaga caaaaagctg ctgcagcagg tagaattccc acaaactatc tgagaagaga
361 ggttggtact tctgataaag aaattctaac aagtcgaata atagatcgtt caattagacc
421 gctctttcca gctggctact tctatgatac acaggttctg tgtaatctgt tagcagtaga
481 tggtgtaaat gagcctgatg tcctagcaat taatggcgct tccgtagccc tctcattatc
541 agatattcct tggaatggac ctgttggggc agtacgaata ggaataattg atggagaata
601 tgttgttaac ccaacaagaa aagaaatgtc ttctagtact ttaaatttag tggttgctgg
661 agcacctaaa agtcagattg tcatgttgga agcctctgca gagaacattt tacagcagga
721 cttttgccat gctatcaaag tgggagtgaa atatacccaa caaataattc agggcattca
781 gcagttggta aaagaaactg gtgttaccaa gaggacacct cagaagttat ttaccccttc
841 gccagagatt gtgaaatata ctcataaact tgctatggag agactctatg cagtttttac
901 agattacgag catgacaaag tttccagaga tgaagctgtt aacaaaataa gattagatac
961 ggaggaacaa ctaaaagaaa aatttccaga agccgatcca tatgaaataa tagaatcctt
1021 caatgttgtt gcaaaggaag tttttagaag tattgttttg aatgaataca aaaggtgcga
1081 tggtcgggat ttgacttcac ttaggaatgt aagttgtgag gtagatatgt ttaaaaccct
1141 tcatggatca gcattatttc aaagaggaca aacacaggtg ctttgtaccg ttacatttga
1201 ttcattagaa tctggtatta agtcagatca agttataaca gctataaatg ggataaaaga
1261 taaaaatttc atgctgcact acgagtttcc tccttatgca actaatgaaa ttggcaaagt
1321 cactggttta aatagaagag aacttgggca tggtgctctt gctgagaaag ctttgtatcc
1381 tgttattccc agagattttc ctttcaccat aagagttaca tctgaagtcc tagagtcaaa
1441 tgggtcatct tctatggcat ctgcatgtgg cggaagttta gcattaatgg attcaggggt
1501 tccaatttca tctgctgttg caggcgtagc aataggattg gtcaccaaaa ccgatcctga
1561 gaagggtgaa atagaagatt atcgtttgct gacagatatt ttgggaattg aagattacaa
1621 tggtgacatg gacttcaaaa tagctggcac taataaagga ataactgcat tacaggctga
1681 tattaaatta cctggaatac caataaaaat tgtgatggag gctattcaac aagcttcagt
1741 ggcaaaaaag gagatattac agatcatgaa caaaactatt tcaaaacctc gagcatctag
1801 aaaagaaaat ggacctgttg tagaaactgt tcaggttcca ttatcaaaac gagcaaaatt
1861 tgttggacct ggtggctata acttaaaaaa acttcaggct gaaacaggtg taactattag
1921 tcaggtggat gaagaaacgt tttctgtatt tgcaccaaca cccagtgcta tgcatgaggc
1981 aagagacttc attactgaaa tctgcaagga tgatcaggag cagcaattag aatttggagc
2041 agtatatacc gccacaataa ctgaaatcag agatactggt gtaatggtaa aattatatcc
2101 aaatatgact gcggtactgc ttcataacac acaacttgat caacgaaaga ttaaacatcc
2161 tactgcccta ggattagaag ttggccaaga aattcaggtg aaatactttg gacgtgaccc
2221 agccgatgga agaatgaggc tttctcgaaa agtgcttcag tcgccagcta caaccgtggt
2281 cagaactttg aatgacagaa gtagtattgt aatgggagaa cctatttcac agtcatcatc
2341 taattctcag tgattttttt tttttaaaga gaattctaga attctatttt gtctagggtg
2401 atgtgctgta gagcaacatt ttagtagtat cttccattgt gtagatttct atataatata
2461 aatacatttt aattatttgt actaaaatgc tcatttacat gtgccatttt tttaattcga
2521 gtaacccata tttgtttaat tgtatttaca ttataaatca agaaatattt attattaaaa
2581 gtaagtcatt tatacatctt agaaaaaatt acatagtttt gtttttacaa ttctgaaata
2641 tatgaaaaac ttagatagaa tatgtcatat gttattataa cagatctctt cctacatctt
2701 atttttcttc tttgtataca gtagtgacag tttaccttca gtcatcctac aggtcacctc
2761 tgtgcccaac aactttactt gcctacaacg gttcacaaac tggaacaggc catattcaaa
2821 gccagtgcct atttcttcag aactgttaac agatatagtg agttgaggga gctaatctga
2881 tacacttttg ataatataat gcctttcaaa ttagttacca aatcataaac agagtggaat
2941 aaatataaat tagattctaa ctaggatgaa tgtggtagta atgatgtata tttttcaaat
3001 ttacctaaac agaaagatta ttggctaagg caggcggatc atgagatcag gagatcgaga
3061 ccatcctggc taacacggtg aaaccccgtc tctactaaaa gtgcaaaaat tagccgggcg
3121 tggtggcgca tgcctgtaat cctagctact caggaggctg aggcagaaga atcacttcaa
3181 cccaggaggt ggaggttgta gtgagccgag attgcgccat tgcactccag ctcaggcaac
3241 aagagcaaaa ctccgtctca aaaaaaaaaa aaaa
//