LOCUS BC053651 2974 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphoinositide-3-kinase, class 3, mRNA (cDNA clone
MGC:61518 IMAGE:5517878), complete cds.
ACCESSION BC053651
VERSION BC053651.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2974)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2974)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: f Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34761063.
FEATURES Location/Qualifiers
source 1..2974
/db_xref="H-InvDB:HIT000053958"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:61518 IMAGE:5517878"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2974
/gene="PIK3C3"
/gene_synonym="Vps34"
/db_xref="GeneID:5289"
/db_xref="HGNC:HGNC:8974"
/db_xref="MIM:602609"
CDS 25..2688
/gene="PIK3C3"
/gene_synonym="Vps34"
/codon_start=1
/product="phosphoinositide-3-kinase, class 3"
/protein_id="AAH53651.1"
/db_xref="GeneID:5289"
/db_xref="HGNC:HGNC:8974"
/db_xref="MIM:602609"
/translation="MGEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPML
KFSGLYQETCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPR
NAQVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTKTP
GRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLM
VEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARS
LRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFL
KCVNWDLPQEAKQALELLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDL
LMYLLQLVQALKYENFDDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPL
PSVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQ
RDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQRESGNR
KKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFK
TEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFM
QFIQSVPVAEVLDTEGSIQNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILG
VGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEF
RKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAV
HYMQSLIDESVHALFAAVVEQIHKFAQYWRK"
BASE COUNT 964 a 539 c 672 g 799 t
ORIGIN
1 ggtggtacct ttgcagacgg tgcgatgggg gaagcagaga agtttcacta catctatagt
61 tgtgacctgg atatcaacgt ccagcttaag ataggaagct tggaagggaa gagagaacaa
121 aagagttata aagctgtcct ggaagaccca atgttgaagt tctcaggact atatcaagag
181 acatgctctg atctttatgt tacttgtcaa gtttttgcag aagggaagcc tttggccttg
241 ccagtgagaa catcctacaa agcatttagt acaagatgga actggaatga atggctgaaa
301 ctaccagtaa aataccctga cctgcccagg aatgcccaag tggccctcac catatgggat
361 gtgtatggtc ccggaaaagc agtgcctgta ggaggaacaa cggtttcgct ctttggaaaa
421 tacggcatgt ttcgccaagg gatgcatgac ttgaaagtct ggcctaatgt agaagcagat
481 ggatcagaac ccacaaaaac tcctggcaga acaagtagca ctctctcaga agatcagatg
541 agccgtcttg ccaagctcac caaagctcat cgacaaggac acatggtgaa agtagattgg
601 ctggatagat tgacatttag agaaatagaa atgataaatg agagtgaaaa acgaagttct
661 aatttcatgt acctgatggt tgaatttcga tgtgtcaagt gtgatgataa ggaatatggt
721 attgtttatt atgaaaagga cggtgatgaa tcatctccaa ttttaacaag ttttgaatta
781 gtgaaagttc ctgaccccca gatgtctatg gagaatttag ttgagagcaa acaccacaag
841 cttgcccgga gtttaagaag tggaccttct gaccacgatc tgaaacccaa tgctgccacg
901 agagatcagt taaatattat tgtgagttat ccaccaacca agcaacttac atatgaagaa
961 caagatcttg tttggaagtt tagatattat cttacgaatc aagaaaaagc cttgacaaaa
1021 ttcttgaaat gtgttaattg ggatctacct caagaggcca aacaggcctt ggaacttctg
1081 ggaaaatgga agccgatgga tgtagaggac tccttggagc tgttatcctc tcattacacc
1141 aacccaactg tgaggcgtta tgctgttgcc cggttgcgac aggccgatga tgaggatttg
1201 ttgatgtacc tattacaatt ggtccaggct ctcaaatatg aaaattttga tgatataaag
1261 aatggattgg aacctaccaa gaaggatagt cagagttcag tgtcagaaaa tgtgtcaaat
1321 tctggaataa attctgcaga aatagatagc tcccaaatta taaccagccc ccttccttca
1381 gtctcttcac ctcctcctgc atcaaaaaca aaagaagttc cagatggcga aaatctggaa
1441 caagatctct gtaccttctt gatatcgaga gcctgcaaaa actcaacact ggctaattat
1501 ttatactggt atgtgatagt ggaatgtgaa gatcaagata ctcagcagag agatccaaag
1561 acccatgaga tgtacttgaa cgtaatgaga agattcagcc aagcattgtt gaagggtgat
1621 aagtctgtca gagttatgcg ttctttgctg gctgcacaac agacatttgt agatcggttg
1681 gtgcatctaa tgaaggcagt acaacgcgaa agtggaaatc gtaagaaaaa gaatgagaga
1741 ctacaggcat tgcttggaga taatgaaaag atgaatttgt cagatgtgga acttatcccg
1801 ttgcctttag aaccccaagt gaaaattaga ggaataattc cggaaacagc tacactgttt
1861 aaaagtgccc ttatgcctgc acagttgttt tttaagacgg aagatggagg caaatatcca
1921 gttatattta agcatggaga tgatttacgt caagatcaac ttattcttca aatcatttca
1981 ctcatggaca agctgttacg gaaagaaaat ctggacttga aattgacacc ttataaggtg
2041 ttagccacca gtacaaaaca tggcttcatg cagtttatcc agtcagttcc tgtggctgaa
2101 gttcttgata cagagggaag cattcagaac ttttttagaa aatatgcacc aagtgagaat
2161 gggccaaatg ggattagtgc tgaggtcatg gacacttacg ttaaaagctg tgctggatat
2221 tgcgtgatca cctatatact tggagttgga gacaggcacc tggataacct tttgctaaca
2281 aaaacaggca aactcttcca catagacttt ggatatattt tgggtcggga tccaaagcct
2341 cttcctccac caatgaagct gaataaagaa atggtagaag gaatgggggg cacacagagt
2401 gagcagtacc aagagttccg taaacagtgt tacacggctt tcctccacct gcgaaggtat
2461 tctaatctga ttttgaactt gttttccttg atggttgatg caaacattcc agatattgca
2521 cttgaaccag ataaaactgt gaaaaaggtt caggataaat tccgcttaga cctgtcggat
2581 gaagaggctg tgcattacat gcagagtctg attgatgaga gtgtccatgc tctttttgct
2641 gcagtggtgg aacagattca caagtttgcc cagtactgga gaaaatgaaa ctgggattga
2701 cccatcaaga tgcttggctc aataagaaaa ccacgttagg agcaaccttt gtatattgga
2761 gacttcagag taaccagcaa ggaagagaaa tcttaatctt caagttacca tattttccaa
2821 atattacatg gtacctgagt tctgcttcct tggatgtcat tgcttaaata tagtcttgaa
2881 gggcttgttt tgaaatattg tatatatttt ttcaaatgta tacattgtta ataaattaag
2941 aaatgagaaa cattcttaaa aaaaaaaaaa aaaa
//