LOCUS BC053646 1993 bp mRNA linear HTC 27-OCT-2008
DEFINITION Homo sapiens eukaryotic translation initiation factor 2B, subunit 5
epsilon, 82kDa, mRNA (cDNA clone IMAGE:5203307), with apparent
retained intron.
ACCESSION BC053646
VERSION BC053646.1
KEYWORDS HTC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1993)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1993)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: d Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33667022
This clone has the following problem: retained intron.
FEATURES Location/Qualifiers
source 1..1993
/db_xref="H-InvDB:HIT000053954"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5203307"
/tissue_type="Lung, Spleen, fetal, pooled"
/clone_lib="NIH_MGC_122"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
BASE COUNT 513 a 459 c 515 g 506 t
ORIGIN
1 gattcccgat ggagctggcc ccttcgtggc gaccacaagt cctatcacgc cacaagaatc
61 tcggcttgtt cacctacaaa tgaaggccga cgatacttgc agaccgagtc tgacatctat
121 gggaccccac tcctaccctg cgcctcaggg gccgggacaa ccgtgacccg taccgactcc
181 tcgcgcctcc gggatcaacc tcagacccct gtgggctccc ggggaagacg catgcgcact
241 gctccctcct cgtctccttc ccttcgttaa ttagtccatc gcatttcaag tgctggatcc
301 tttttgtccc ctactgcgtg cggtggcagc ttccttgcgg aagtggtgac cgtgagagaa
361 gaagatggcg gcccctgtag tggcgccgcc tggtgtggtg gttagtcggg ctaacaagcg
421 cagcggcgcg gggccgggag gcagcggtgg cgggggagcc agaggggcgg aggaggaacc
481 gccgccgccc ctacaagcag ttctggtggc cgatagcttc gatcgccgct tcttccccat
541 ctccaaggac cagcctcggg tgagcgccgc gcacgcgagc agccagaggg caggaagggt
601 ggcagggctg gagcaagttc tcatttcggc taactacaac agcatgccct tgaaggacct
661 gcgtctatgc tgttatccta aaaccggatg cagagggact gtctaaaccc tgatgactgc
721 tgaccaagtt agtggccggc agatttgggc attcgtaatg ctgtccacct cgggtttgaa
781 aggaatgaag agagctaaat gtagaaacag cgcttgagag aagccttcac gcggggattc
841 agtgccccta gaagaggctg cgccgctttt aaccttgccg atgacggttg cttaggtgaa
901 aagaaacgac cttttgcctc tgaaagggcg gtaatatgcc ttaaaaagag cgggctagat
961 ctcagacagt gtattttttt tttccttcca ggttctaatg atagccggtg ctatctgtcg
1021 aaaggacaaa atgggtttag tggttgattg ccatcagccc ccgagttgtc tagacaatgg
1081 ggtattggac tcagctgggt cgttcagcag tcagttcaga aagagggaac tgcattggat
1141 ttaaggaaaa tcctctctaa ttccctcgaa aaagcctaat ggctgttcat ttttgacaag
1201 tgacgggaga tggctaacct ctgacactta gaaagggatc aaggaaggaa atcttatggg
1261 gaagaaggcc ttagcaatgc ttaaatttat ggaaatatgg ttgatttcca gtattatcct
1321 ttgccagcag tgaactgcca ttctgtcaca gctctgtgtc tgatacaagg cacatagatt
1381 ctgtacttac catccccaaa ttgcaatgtc tcagactcag gcttagagca tggcatgaac
1441 atcaaaggca ggaacctgtt tatctttgaa ttggaaagat acagcaaaat tacactgttt
1501 ggaaatacga atagaggagt gaaaattgtt gcagtggggt acccaaaggg attcgagacc
1561 tcaagttttt tttcatcttg tatccttcag gtcctcttgc ccctggccaa tgtggcatta
1621 attgactaca ctctggaatt cctgactgcc acaggtgtac aggaaacatt tgtcttttgt
1681 tgctggaaag ctgctcaaat caaagaacat ttactgtaag gccctgcaac ttttctttcc
1741 atgtttcgcc atctttttcc agttttttca ggatgaatgt aactaagggg aaatgtgaga
1801 ggggaaaaat gtgtctactt agatgtcatt gtaagttcta agggtatttt ctacacttat
1861 caggaggtga gcagctgaaa ctttgtatct gtctgtcttg ggttttctgt gacaaagatt
1921 aaagaataaa cttgggtttt ttctgaatct cattataaaa aaaaaaaaaa aaaaaaaaaa
1981 aaaaaaaaaa aaa
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