LOCUS BC053626 3521 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens midline 1 (Opitz/BBB syndrome), mRNA (cDNA clone
MGC:61495 IMAGE:6163881), complete cds.
ACCESSION BC053626
VERSION BC053626.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3521)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3521)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: m Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4557752.
FEATURES Location/Qualifiers
source 1..3521
/db_xref="H-InvDB:HIT000053946"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:61495 IMAGE:6163881"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3521
/gene="MID1"
/gene_synonym="BBBG1"
/gene_synonym="FXY"
/gene_synonym="GBBB1"
/gene_synonym="OGS1"
/gene_synonym="OS"
/gene_synonym="OSX"
/gene_synonym="RNF59"
/gene_synonym="TRIM18"
/gene_synonym="XPRF"
/gene_synonym="ZNFXY"
/db_xref="GeneID:4281"
/db_xref="HGNC:HGNC:7095"
/db_xref="MIM:300552"
CDS 259..2262
/gene="MID1"
/gene_synonym="BBBG1"
/gene_synonym="FXY"
/gene_synonym="GBBB1"
/gene_synonym="OGS1"
/gene_synonym="OS"
/gene_synonym="OSX"
/gene_synonym="RNF59"
/gene_synonym="TRIM18"
/gene_synonym="XPRF"
/gene_synonym="ZNFXY"
/codon_start=1
/product="midline 1 (Opitz/BBB syndrome)"
/protein_id="AAH53626.1"
/db_xref="GeneID:4281"
/db_xref="HGNC:HGNC:7095"
/db_xref="MIM:300552"
/translation="METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCAT
NESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRR
ERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHR
LIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDK
LKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQR
RQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKN
ITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELC
TASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTV
HGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVER
DESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHE
WIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSI
HLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP"
BASE COUNT 971 a 839 c 787 g 924 t
ORIGIN
1 gtctcgagca gagtgcgtgt agcaacagat caaagaaaaa gaggacgaaa acgtgctctt
61 tgctgcccgt agatttcgcc gggttgcttt tgtcttgcgg gctcctgtcg ggttcggtgt
121 ttccgctctg aagactgcga cgcgggctcc gatgcagctc gctccctgcc ggatgggtca
181 tgggattcta aacatgaggc agatagctga tcagcttcct tgggttttgc tgatgacaca
241 agagagcttt gcctgaagat ggaaacactg gagtcagaac tgacctgccc tatttgtctg
301 gagctctttg aggaccctct tctactgccc tgcgcacaca gcctctgctt caactgcgcc
361 caccgcatcc tagtatcaca ctgtgccacc aacgagtctg tggagtccat caccgccttc
421 cagtgcccca cctgccggca tgtcatcacc ctcagccagc gaggtctaga cgggctcaag
481 cgcaacgtca ccctacagaa catcatcgac aggttccaga aagcatcagt gagcgggccc
541 aactctccca gcgagacccg tcgggagcgg gcctttgacg ccaacaccat gacctccgcc
601 gagaaggtcc tctgccagtt ttgtgaccag gatcctgccc aggacgctgt gaagacctgt
661 gtcacttgtg aagtatccta ctgtgacgag tgcctgaaag ccactcaccc gaataagaag
721 ccctttacag gccatcgtct gattgagcca attccggact ctcacatccg ggggctgatg
781 tgcttggagc atgaggatga gaaggtgaat atgtactgtg tgaccgatga ccagttaatc
841 tgtgccttgt gtaaactggt tgggcggcac cgcgatcatc aggtggcagc tttgagtgag
901 cgctatgaca aattgaagca aaacttagag agtaacctca ccaaccttat taagaggaac
961 acagaactgg agaccctttt ggctaaactc atccaaacct gtcaacatgt tgaagtcaat
1021 gcatcacgtc aagaagccaa attgacagag gagtgtgatc ttctcattga gatcattcag
1081 caaagacgac agattattgg aaccaagatc aaagaaggga aggtgatgag gcttcgcaaa
1141 ctggctcagc agattgcaaa ctgcaaacag tgcattgagc ggtcagcatc actcatctcc
1201 caagcggaac actctctgaa ggagaatgat catgcgcgtt tcctacagac tgctaagaat
1261 atcaccgaga gagtctccat ggcaactgca tcctcccagg ttctaattcc tgaaatcaac
1321 ctcaatgaca catttgacac ctttgcctta gatttttccc gagagaagaa actgctagaa
1381 tgtctggatt accttacagc tcccaaccct cccacaatta gagaagagct ctgcacagct
1441 tcatatgaca ccatcactgt gcattggacc tccgatgatg agttcagcgt ggtctcctac
1501 gagctccagt acaccatatt caccggacaa gccaacgtcg ttagtctgtg taattcggct
1561 gatagctgga tgatagtacc caacatcaag cagaaccact acacggtgca cggtctgcag
1621 agcggcacca agtacatctt catggtcaag gccatcaacc aggcgggcag ccgcagcagt
1681 gagcctggga agttgaagac aaacagccaa ccatttaaac tggatcccaa atctgctcat
1741 cgaaaactga aggtgtccca tgataacttg acagtagaac gtgatgagtc atcatccaag
1801 aagagtcaca cacctgaacg cttcaccagc caggggagct atggagtagc tggaaatgtg
1861 tttattgata gtggccggca ttattgggaa gtggtcataa gtggaagcac atggtatgcc
1921 attggtcttg cttacaaatc agccccgaag catgaatgga ttgggaagaa ctctgcttcc
1981 tgggcgctct gccgctgcaa caataactgg gtggtgagac acaatagcaa ggaaatcccc
2041 attgagcctg ccccccacct ccggcgcgtg ggcatcctgc tggactatga taacggctct
2101 atcgcctttt atgatgcttt gaactccatc cacctctaca ccttcgacgt cgcatttgcg
2161 cagcctgttt gccccacctt caccgtgtgg aacaagtgtc tgacgattat cactgggctc
2221 cctatcccag accatttgga ctgcacagag cagctgccgt gagcgtctgg ccacatggag
2281 ctgctttctg gggaacagta aggttcaggc cactatttag gggactgaga aagcacaggc
2341 ttcatgagtg taatgaaatc tcaccagaag tgtcccgaaa tcggctcaga tagggctcaa
2401 aacaagagat tcctctcctt ttactgtgtc ttgtattaag tacgggcttt aataatttct
2461 ttaatttttt tgtatttaga ggaaaatcta tagattattt ataagagaaa cataatcagg
2521 attacaactt ttaggaatta cttggttttg cacattaaga ggcccataag tttatcagct
2581 atttacaacc ttcatttcat cacaatctgt gggcttacaa aaaaacaaaa acttttgtag
2641 ttttgtatgt tactcatctt cttacctgat atcccatgat gatcccatgg taggtcttct
2701 cacctcgatg gtgcataaca ggatgtgttt gaacctagta ggggaggaaa caggctttct
2761 tactctggtt taatttgaag tgttttaatt gtgatgtcaa aaagttgtat cagatcaact
2821 aaaatggaga gcaagacaga gaatgaaaag agttgatttt ggacctcgga ccttgccgtg
2881 gctaaatctt taccttctca tagctgatgg gataatgttg gaaagaaagg ttctgaatcc
2941 tttggccaca ttttgccctg cttctctcag ggttaagggt tctggaagaa cattaagaat
3001 gagattgcaa ttgaaaatag tcattttgaa tcctattgat tattcaaaaa ttcaggctga
3061 tttgtctttt atcagaggta ggattctgtt ttatagtata gaatctactt tatccttcct
3121 tttaatagtt cctttagacc tgtgaaattt cttcactaca tttaatagtt ctcctatttc
3181 ccgctccccc atatcaattt tccttttgtc tccggggctg agtaaataaa catgttctgt
3241 cacaaatagc agcaccactt tggattgatt ttgctctcca ggacatcagc acatggccct
3301 gatcagcact accacatcca aacataagtc actgaaaaac acttaatatt tatgagttgg
3361 taatgacaag ggacattgta taaagtacta tttgctagat tcatgcctca aaagttatta
3421 taaacagacc tttattaaac acatcttgaa agatgtagaa gtccctctat agtctagtat
3481 agtttacaat agagttgtaa gaccaaaaaa aaaaaaaaaa a
//