LOCUS BC053608 1570 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens nuclear autoantigenic sperm protein (histone-binding),
mRNA (cDNA clone MGC:61522 IMAGE:6013263), complete cds.
ACCESSION BC053608
VERSION BC053608.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1570)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1570)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: i Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 27262627.
FEATURES Location/Qualifiers
source 1..1570
/db_xref="H-InvDB:HIT000053938"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:61522 IMAGE:6013263"
/tissue_type="Testis, embryonal carcinoma"
/clone_lib="NIH_MGC_92"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1570
/gene="NASP"
/gene_synonym="DKFZp547F162"
/gene_synonym="FLB7527"
/gene_synonym="FLJ31599"
/gene_synonym="FLJ35510"
/gene_synonym="MGC19722"
/gene_synonym="MGC20372"
/gene_synonym="MGC2297"
/gene_synonym="PRO1999"
/db_xref="GeneID:4678"
/db_xref="HGNC:HGNC:7644"
/db_xref="MIM:603185"
CDS 83..1432
/gene="NASP"
/gene_synonym="DKFZp547F162"
/gene_synonym="FLB7527"
/gene_synonym="FLJ31599"
/gene_synonym="FLJ35510"
/gene_synonym="MGC19722"
/gene_synonym="MGC20372"
/gene_synonym="MGC2297"
/gene_synonym="PRO1999"
/codon_start=1
/product="nuclear autoantigenic sperm protein
(histone-binding)"
/protein_id="AAH53608.1"
/db_xref="GeneID:4678"
/db_xref="HGNC:HGNC:7644"
/db_xref="MIM:603185"
/translation="MAMESTATAAVAAELVSADKIEDVPAPSTSADKVESLDVDSEAK
KLLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARME
NGVLGNALEGVHVEEEEGEKTEDESLVENNDNIDETEGSEEDDKENDKTEEMPNDSVL
ENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESEN
YVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIE
NRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKAT
LVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDD
AKKAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQAESRAAVEGTVEAGATVESTAC"
BASE COUNT 503 a 280 c 434 g 353 t
ORIGIN
1 tgagtgagtc tctggcgtcc caaattgcct gtttttctcg caggctctat tccgttcgct
61 ggttcgccac ctcaggggaa cgatggccat ggagtccaca gccactgccg ccgtcgccgc
121 ggagctggtt tctgccgaca aaattgaaga tgttcctgct ccttctacat ctgcagataa
181 agtggagagt ctggatgtgg atagtgaagc taagaaacta ttgggtttag gacagaaaca
241 tctggtgatg ggggatattc cagcagctgt caatgcattc caggaagcag ctagtctttt
301 aggtaagaag tatggagaga cagctaatga gtgtggagaa gccttctttt tctatgggaa
361 atcacttctg gagttggcaa gaatggagaa tggtgtgttg ggaaacgcct tggaaggtgt
421 gcatgtggaa gaggaagaag gagaaaaaac agaagatgaa tctctggtag aaaataatga
481 taacatagat gaaactgaag gctcagaaga ggatgataaa gaaaatgata agaccgaaga
541 aatgccaaat gattcagtcc ttgaaaacaa gtctcttcaa gaaaatgagg aggaggagat
601 tgggaaccta gagcttgcct gggatatgct ggatttagca aagatcattt ttaaaaggca
661 agaaacaaaa gaagcacagc tttatgctgc ccaggcacat cttaaactcg gagaagttag
721 tgttgaatct gaaaactatg tgcaagctgt ggaggagttc cagtcctgcc ttaacctgca
781 ggaacagtac ctggaagccc acgaccgtct ccttgcagag acccactacc agctgggctt
841 ggcttatggg tacaactctc agtatgatga ggcagtggca cagttcagca aatctattga
901 agtcattgag aacagaatgg ctgtactaaa cgagcaggtg aaggaggctg aaggatcgtc
961 tgctgaatac aagaaagaaa ttgaggaact aaaggaactg ctacccgaaa ttagagagaa
1021 gatagaagat gcaaaggagt ctcagcgtag tgggaatgta gctgaactgg ctctgaaagc
1081 tactctggtg gagagttcta cttcaggttt cactcctggt ggaggaggct cttcagtctc
1141 catgattgcc agtagaaagc caacagacgg tgcttcctca tcaaattgtg tgactgatat
1201 ttcccacctt gtcagaaaga agaggaaacc agaggaagag agtccccgga aagatgatgc
1261 aaagaaagcc aaacaagagc cggaggtgaa cggaggcagt ggggatgctg tccccagtgg
1321 aaatgaagtt tcggaaaaca tggaggagga ggctgagaat caggctgaaa gccgggcagc
1381 agtggagggg acagtggagg ctggagctac agttgaaagc actgcatgtt aagagggggc
1441 acagccctcc tcccaaggga aagtgttttt gtatataatg tattttttca cttttggagg
1501 attctttttg tataacttca ataaagattg taagcaaaaa aaaaaaaaaa aaaaaaaaaa
1561 aaaaaaaaaa
//