LOCUS BC053321 1748 bp mRNA linear HUM 18-MAY-2004
DEFINITION Homo sapiens protein kinase C, alpha, mRNA (cDNA clone
IMAGE:6538304), partial cds.
ACCESSION BC053321
VERSION BC053321.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1748)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1748)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (02-JUN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 47 Row: m Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4506066.
FEATURES Location/Qualifiers
source 1..1748
/db_xref="H-InvDB:HIT000259247"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6538304"
/tissue_type="Lung, epidermoid carcinoma"
/clone_lib="NIH_MGC_101"
/lab_host="DH10B"
/note="Vector: pOTB7"
gene <1..1748
/gene="PRKCA"
/gene_synonym="PKC-alpha"
/gene_synonym="PKCA"
/gene_synonym="PRKACA"
/db_xref="GeneID:5578"
/db_xref="MIM:176960"
CDS <1..1143
/gene="PRKCA"
/gene_synonym="PKC-alpha"
/gene_synonym="PKCA"
/gene_synonym="PRKACA"
/codon_start=1
/product="PRKCA protein"
/protein_id="AAH53321.1"
/db_xref="GeneID:5578"
/db_xref="MIM:176960"
/translation="ASQRLRLPLPLRAAPARLSPAPAAPPRRDLGHRPAPRARGRPEP
APLPRRPRPPGPPRPQLPGGGKRWLGGTMADVFPGNDSTASQDVANRFARKGALRQKN
VHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPG
ADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVIN
VPSLCGMDHTEKRGRIYLKAEVADEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDP
KNESKQKTKTIRSTLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFG
VSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNMELRQKFEWR"
BASE COUNT 475 a 472 c 446 g 355 t
ORIGIN
1 gcgagccagc ggctccggct cccgctcccg ctccgcgcag caccagcccg actctccccg
61 gcccccgccg cgccccctcg ccgcgacctc ggccaccggc ccgcgccccg cgcccggggt
121 cgccccgagc ccgcacctct cccccgccgc ccccgcccac ccggccctcc gcggccgcag
181 ctccccggcg gaggcaagag gtggttgggg gggaccatgg ctgacgtttt cccgggcaac
241 gactccacgg cgtctcagga cgtggccaac cgcttcgccc gcaaaggggc gctgaggcag
301 aagaacgtgc acgaggtgaa ggaccacaaa ttcatcgcgc gcttcttcaa gcagcccacc
361 ttctgcagcc actgcaccga cttcatctgg gggtttggga aacaaggctt ccagtgccaa
421 gtttgctgtt ttgtggtcca caagaggtgc catgaatttg ttactttttc ttgtccgggt
481 gcggataagg gacccgacac tgatgacccc aggagcaagc acaagttcaa aatccacact
541 tacggaagcc ccaccttctg cgatcactgt gggtcactgc tctatggact tatccatcaa
601 gggatgaaat gtgacacctg cgatatgaac gttcacaagc aatgcgtcat caatgtcccc
661 agcctctgcg gaatggatca cactgagaag agggggcgga tttacctaaa ggctgaggtt
721 gctgatgaaa agctccatgt cacagtacga gatgcaaaaa atctaatccc tatggatcca
781 aacgggcttt cagatcctta tgtgaagctg aaacttattc ctgatcccaa gaatgaaagc
841 aagcaaaaaa ccaaaaccat ccgctccaca ctaaatccgc agtggaatga gtcctttaca
901 ttcaaattga aaccttcaga caaagaccga cgactgtctg tagaaatctg ggactgggat
961 cgaacaacaa ggaatgactt catgggatcc ctttcctttg gagtttcgga gctgatgaag
1021 atgccggcca gtggatggta caagttactt aaccaagaag aaggtgagta ctacaacgta
1081 cccattccgg aaggggacga ggaaggaaac atggaactca ggcagaaatt cgagtggaga
1141 tgataataga gactgccgca taggactgct gggaggattc actcagttca cttacgtcag
1201 gatcatccat atggctgtcc ctcacctctg catcttgtcc cactggaagg tcttcagggg
1261 cagtaataca catggagctg tcatcacccc tgataacaat gccttctgga atacctcctg
1321 aaggctctgc ctaaggctgt tctacagtta acttttgttt taataagtag aaaaaaataa
1381 gacctgatgt ggtggctcat gcctataatc ccagcaagtt tgccaggcca aggcgggtgg
1441 atcacctgag gttaggagtt caagaccagc tgggccaaca tggtgaaacc gcatctctgc
1501 taaaaataca aaaattagcc aggcatggtg gtgagagctt gtggtcccag ctacttggga
1561 ggctgaggca gtagaatcac ttgagcccgg gaggtggagg ttgcagtgag ctgggatcgt
1621 gccattgcat tccagcctgg gtgacagacc gagactccgt ctcaagaaaa aaaaaaaaat
1681 agaaaaaata tactcttaag ataacaataa aaatatagta tagtgaatac aaaaaaaaaa
1741 aaaaaaaa
//