LOCUS BC052807 1126 bp mRNA linear HUM 30-SEP-2003
DEFINITION Homo sapiens NIMA (never in mitosis gene a)-related kinase 2, mRNA
(cDNA clone IMAGE:5416586), partial cds.
ACCESSION BC052807
VERSION BC052807.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1126)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1126)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (27-MAY-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 115 Row: e Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4505372.
FEATURES Location/Qualifiers
source 1..1126
/db_xref="H-InvDB:HIT000259220"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5416586"
/tissue_type="Breast, mammary adenocarcinoma."
/clone_lib="NIH_MGC_87"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..>1126
/gene="NEK2"
/gene_synonym="HsPK 21"
/gene_synonym="HsPK21"
/gene_synonym="NLK1"
/db_xref="GeneID:4751"
/db_xref="MIM:604043"
CDS 147..>1126
/gene="NEK2"
/gene_synonym="HsPK 21"
/gene_synonym="HsPK21"
/gene_synonym="NLK1"
/codon_start=1
/product="NEK2 protein"
/protein_id="AAH52807.1"
/db_xref="GeneID:4751"
/db_xref="MIM:604043"
/translation="MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSM
TEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGT
KERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDF
GLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTA
FSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVA
DEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAKKKK"
misc_feature 168..959
/gene="NEK2"
/gene_synonym="HsPK 21"
/gene_synonym="HsPK21"
/gene_synonym="NLK1"
/note="S_TKc; Region: Serine/Threonine protein kinases,
catalytic domain"
/db_xref="CDD:smart00220"
BASE COUNT 336 a 225 c 294 g 271 t
ORIGIN
1 ggttaaacgg ggcccaaggc aggggtggcg ggtcagtgct gctcgggggc ttctccatcc
61 aggtccctgg agttcctggt ccctggagct ccgcacttgg cggcgcaacc tgcgtgaggc
121 agcgcgactc tggcgactgg ccggccatgc cttcccgggc tgaggactat gaagtgttgt
181 acaccattgg cacaggctcc tacggccgct gccagaagat ccggaggaag agtgatggca
241 agatattagt ttggaaagaa cttgactatg gctccatgac agaagctgag aaacagatgc
301 ttgtttctga agtgaatttg cttcgtgaac tgaaacatcc aaacatcgtt cgttactatg
361 atcggattat tgaccggacc aatacaacac tgtacattgt aatggaatat tgtgaaggag
421 gggatctggc tagtgtaatt acaaagggaa ccaaggaaag gcaatactta gatgaagagt
481 ttgttcttcg agtgatgact cagttgactc tggccctgaa ggaatgccac agacgaagtg
541 atggtggtca taccgtattg catcgggatc tgaaaccagc caatgttttc ctggatggca
601 agcaaaacgt caagcttgga gactttgggc tagctagaat attaaaccat gacacgagtt
661 ttgcaaaaac atttgttggc acaccttatt acatgtctcc tgaacaaatg aatcgcatgt
721 cctacaatga gaaatcagat atctggtcat tgggctgctt gctgtatgag ttatgtgcat
781 taatgcctcc atttacagct tttagccaga aagaactcgc tgggaaaatc agagaaggca
841 aattcaggcg aattccatac cgttactctg atgaattgaa tgaaattatt acgaggatgt
901 taaacttaaa ggattaccat cgaccttctg ttgaagaaat tcttgagaac cctttaatag
961 cagatttggt tgcagacgag caaagaagaa atcttgagag aagagggcga caattaggag
1021 agccagaaaa atcgcaggat tccagccctg tattgagtga gctgaaactg aaggaaattc
1081 agttacagga gcgagagcga gctctcaaag caaaaaaaaa aaaaaa
//