LOCUS BC051338 5011 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens endothelial PAS domain protein 1, mRNA (cDNA clone
MGC:59860 IMAGE:6305604), complete cds.
ACCESSION BC051338
VERSION BC051338.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5011)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5011)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-APR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 47 Row: k Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41327154.
FEATURES Location/Qualifiers
source 1..5011
/db_xref="H-InvDB:HIT000053632"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:59860 IMAGE:6305604"
/tissue_type="Eye, normal, pigmented retinal epithelium"
/clone_lib="NIH_MGC_43"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..5011
/gene="EPAS1"
/gene_synonym="HIF2A"
/gene_synonym="HLF"
/gene_synonym="MOP2"
/gene_synonym="PASD2"
/db_xref="GeneID:2034"
/db_xref="HGNC:HGNC:3374"
/db_xref="MIM:603349"
CDS 316..2928
/gene="EPAS1"
/gene_synonym="HIF2A"
/gene_synonym="HLF"
/gene_synonym="MOP2"
/gene_synonym="PASD2"
/codon_start=1
/product="endothelial PAS domain protein 1"
/protein_id="AAH51338.1"
/db_xref="GeneID:2034"
/db_xref="HGNC:HGNC:3374"
/db_xref="MIM:603349"
/translation="MTADKEKKRSSSERRKEKSRDAARCRRSKETEVFYELAHELPLP
HSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVV
TQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSK
DMSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVKVYNNCPPHNSLCGYKEPL
LSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELIGYHPEELLGRS
AYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQP
QCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLMAMNSIFDSSGKGAVSEKSNFLFTK
LKEEPEELAQLAPTPGDAIISLDFGNQNFEESSAYGKAILPPSQPWATELRSHSTQSE
AGSLPAFTVPQAAAPGSTTPSATSSSSSCSTPNSPEDYYTSLDNDLKIEVIEKLFAMD
TEAKDQCSTQTDFNELDLETLAPYIPMDGEDFQLSPICPEERLLAENPQSTPQHCFSA
MTNIFQPLAPVAPHSPFLLDKFQQQLESKKTEPEHRPMSSIFFDAGSKASLPPCCGQA
STPLSSMGGRSNTQWPPDPPLHFGPTKWAVGDQRTEFLGAAPLGPPVSPPHVSTFKTR
SAKGFGARGPDVLSPAMVALSNKLKLKRQLEYEEQAFQDLSGGDPPGGSTSHLMWKRM
KNLRGGSCPLMPDKPLSANVPNDKFTQNPMRGLGHPLRHLPLPQPPSAISPGENSKSR
FPPQCYATQYQDYSLSSAHKVSGMASRLLGPSFESYLLPELTRYDCEVNVPVLGSSTL
LQGGDLLRALDQAT"
BASE COUNT 1248 a 1383 c 1213 g 1167 t
ORIGIN
1 cttttccagg gaaaaaggaa cttgggttcc cttctctccg tcctcttttc gggtctgaca
61 gcctccaccc actccttccc cggaccccgc ctccgcgcgc aggttcctcc cagtcacctt
121 tctccacccc cgcccccgca cctagcccgc cgcgcgccac cttccacctg actgcgcggg
181 gcgctcggga cctgcgcgca cctcggacct tcaccacccg cccgggccgc ggggagcgga
241 cgagggccac agccccccac ccgccaggga gcccaggtgc tcggcgtctg aacgtctcaa
301 agggccacag cgacaatgac agctgacaag gagaagaaaa ggagtagctc ggagaggagg
361 aaggagaagt cccgggatgc tgcgcggtgc cggcggagca aggagacgga ggtgttctat
421 gagctggccc atgagctgcc tctgccccac agtgtgagct cccatctgga caaggcctcc
481 atcatgcgac tggcaatcag cttcctgcga acacacaagc tcctctcctc agtttgctct
541 gaaaacgagt ccgaagccga agctgaccag cagatggaca acttgtacct gaaagccttg
601 gagggtttca ttgccgtggt gacccaagat ggcgacatga tctttctgtc agaaaacatc
661 agcaagttca tgggacttac acaggtggag ctaacaggac atagtatctt tgacttcact
721 catccctgcg accatgagga gattcgtgag aacctgagtc tcaaaaatgg ctctggtttt
781 gggaaaaaaa gcaaagacat gtccacagag cgggacttct tcatgaggat gaagtgcacg
841 gtcaccaaca gaggccgtac tgtcaacctc aagtcagcca cctggaaggt cttgcactgc
901 acgggccagg tgaaagtcta caacaactgc cctcctcaca atagtctgtg tggctacaag
961 gagcccctgc tgtcctgcct catcatcatg tgtgaaccaa tccagcaccc atcccacatg
1021 gacatccccc tggatagcaa gaccttcctg agccgccaca gcatggacat gaagttcacc
1081 tactgtgatg acagaatcac agaactgatt ggttaccacc ctgaggagct gcttggccgc
1141 tcagcctatg aattctacca tgcgctagac tccgagaaca tgaccaagag tcaccagaac
1201 ttgtgcacca agggtcaggt agtaagtggc cagtaccgga tgctcgcaaa gcatgggggc
1261 tacgtgtggc tggagaccca ggggacggtc atctacaacc ctcgcaacct gcagccccag
1321 tgcatcatgt gtgtcaacta cgtcctgagt gagattgaga agaatgacgt ggtgttctcc
1381 atggaccaga ctgaatccct gttcaagccc cacctgatgg ccatgaacag catctttgat
1441 agcagtggca agggggctgt gtctgagaag agtaacttcc tattcaccaa gctaaaggag
1501 gagcccgagg agctggccca gctggctccc accccaggag acgccatcat ctctctggat
1561 ttcgggaatc agaacttcga ggagtcctca gcctatggca aggccatcct gcccccgagc
1621 cagccatggg ccacggagtt gaggagccac agcacccaga gcgaggctgg gagcctgcct
1681 gccttcaccg tgccccaggc agctgccccg ggcagcacca cccccagtgc caccagcagc
1741 agcagcagct gctccacgcc caatagccct gaagactatt acacatcttt ggataacgac
1801 ctgaagattg aagtgattga gaagctcttc gccatggaca cagaggccaa ggaccaatgc
1861 agtacccaga cggatttcaa tgagctggac ttggagacac tggcacccta tatccccatg
1921 gacggggaag acttccagct aagccccatc tgccccgagg agcggctctt ggcggagaac
1981 ccacagtcca ccccccagca ctgcttcagt gccatgacaa acatcttcca gccactggcc
2041 cctgtagccc cgcacagtcc cttcctcctg gacaagtttc agcagcagct ggagagcaag
2101 aagacagagc ccgagcaccg gcccatgtcc tccatcttct ttgatgccgg aagcaaagca
2161 tccctgccac cgtgctgtgg ccaggccagc acccctctct cttccatggg gggcagatcc
2221 aatacccagt ggcccccaga tccaccatta cattttgggc ccacaaagtg ggccgtcggg
2281 gatcagcgca cagagttctt gggagcagcg ccgttggggc cccctgtctc tccaccccat
2341 gtctccacct tcaagacaag gtctgcaaag ggttttgggg ctcgaggccc agacgtgctg
2401 agtccggcca tggtagccct ctccaacaag ctgaagctga agcgacagct ggagtatgaa
2461 gagcaagcct tccaggacct gagcgggggg gacccacctg gtggcagcac ctcacatttg
2521 atgtggaaac ggatgaagaa cctcaggggt gggagctgcc ctttgatgcc ggacaagcca
2581 ctgagcgcaa atgtacccaa tgataagttc acccaaaacc ccatgagggg cctgggccat
2641 cccctgagac atctgccgct gccacagcct ccatctgcca tcagtcccgg ggagaacagc
2701 aagagcaggt tccccccaca gtgctacgcc acccagtacc aggactacag cctgtcgtca
2761 gcccacaagg tgtcaggcat ggcaagccgg ctgctcgggc cctcatttga gtcctacctg
2821 ctgcccgaac tgaccagata tgactgtgag gtgaacgtgc ccgtgctggg aagctccacg
2881 ctcctgcaag gaggggacct cctcagagcc ctggaccagg ccacctgagc caggccttct
2941 acctgggcag cacctctgcc gacgccgtcc caccagcttc actctctccg tctgtttttg
3001 caactaggta tttctaacgc cagcacacta tttacaagat ggacttacct ggcagacttg
3061 cccaggtcac caagcagtgg cctttttctg agatgctcac tttattatcc ctatttttaa
3121 agtacacaat tgttttacct gttctgaaat gttcttaaat tttgtaggat ttttttcctc
3181 cccaccttca atgacttcta atttatatta tccataggtt tctctccctc cttctccttc
3241 tcacacacaa ctgtccatac taacaagttt ggtgcatgtc tgttcttctg tagggagaag
3301 ctttagcttc attttactaa aaagattcct cgttattgtt gttgccaaag agaaacaaaa
3361 atgattttgc tttccaagct tggtttgtgg cgtctccctc gcagagccct tctcgtttct
3421 tttttaaact aatcaccata ttgtaaattt cagggttttt ttttttgttt aagctgactc
3481 tttgctctaa ttttggaaaa aaagaaatgt gaagggtcaa ctccaacgta tgtggttatc
3541 tgtgaaagtt gcacagcgtg gcttttccta aactggtgtt tttcccccgc atttggtgga
3601 ttttttatta ttattcaaaa acataactga gttttttaaa agaggagaaa atttatatct
3661 gggttaagtg tttatcatat atatgggtac tttgtaatat ctaaaaactt agaaacggaa
3721 atggaatcct gctcacaaaa tcactttaag atcttttcga agctgttaat ttttcttagt
3781 gttgtggaca ctgcagactt gtccagtgct cccacggcct gtacggacac tgtggaaggc
3841 ctccctctgt cggctttttg ccatctgtga tatgccatag gtgtgacaat ccgagcagtg
3901 gagtcattca gcgggagcac tgcgcgctat cccctcacat tctctatgta ctatgtatgt
3961 atgtattatt attattgctg ccaagagggt ctgatggcac gttgtggggt cggggggtgg
4021 ggcggggaag tgctctaact tttcttaagg ttttgttgct agcccttcaa gtgcactgag
4081 ctatgtgact cggatggtct ttcacacggc acatttggac atttccagaa ctaccatgag
4141 atggtttaga cgggaattca tgcaaatgag gggtcaaaaa tggtatagtg accccgtcca
4201 cgtcctccaa gctcacgacc ttggagcccc gtggagctgg actgaggagg aggctgcaca
4261 gcgggagagc agctggtcca gaccagccct gcagccccca ctcagccggc agccagatgg
4321 ccccgcaagg cctccaggga tggcccctag ccacaggccc tggctgaggt ctctgggtcg
4381 gtcagtgaca tgtaggtagg aagcactgaa aatagtgttc ccagagcact ttgcaactcc
4441 ctgggtaaga gggacgacac ctctggtttt tcaataccaa ttacatggaa cttttctgta
4501 atgggtacaa tgaagaagtt tctaaaaaca cacacaaagc acattgggcc aactatttag
4561 taagcccgga tagacttatt gccaaaaaca aaaaatagct ttcaaaagaa atttaagttc
4621 tatgagaaat tccttagtca tggtgttgcg taaatcatat tttagctgca cggcattacc
4681 ccacacaggg tggcagaact tgaagggtta ctgacgtgta aatgctggta tttgatttcc
4741 tgtgtgtgtt gccctggcat taagggcatt ttacccttgc agttttacta aaacactgaa
4801 aaatattcca agcttcatat taaccctacc tgtcaacgta acgatttcat gaacgttatt
4861 atattgtcga attcctactg acaacattat aactgtatgg gagcttaact ttataaggaa
4921 atgtattttg acactggtat cttattaaag tattctgatc ctaaaaaaaa aaaaaaaaaa
4981 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//