LOCUS BC051278 3136 bp mRNA linear HUM 03-JAN-2005
DEFINITION Homo sapiens apolipoprotein B (including Ag(x) antigen), mRNA (cDNA
clone IMAGE:6270815), complete cds.
ACCESSION BC051278
VERSION BC051278.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3136)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3136)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (25-APR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CGAP (Stanford)
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 47 Row: i Column: 1
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..3136
/db_xref="H-InvDB:HIT000053599"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6270815"
/tissue_type="Liver, hepatocellular carcinoma"
/clone_lib="NIH_MGC_100"
/lab_host="DH10B"
/note="Vector: pOTB7"
gene 1..3136
/gene="APOB"
/gene_synonym="FLDB"
/db_xref="GeneID:338"
/db_xref="MIM:107730"
CDS 18..2495
/gene="APOB"
/gene_synonym="FLDB"
/codon_start=1
/product="APOB protein"
/protein_id="AAH51278.1"
/db_xref="GeneID:338"
/db_xref="MIM:107730"
/translation="MDPPRPALLALPALLLLLLAGARAEEEMLENVSLVCPKDATRFK
HLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCILKEVYGF
NPEGKALLKKTKNSEEFAAAMSRYELKLAIPEGKQVFLYPEKDEPTYILNIKRGIISA
LLVPPETEEAKQVLFLDTVYGNCSTHFTVKTRKGNVATEISTERDLGQCDRFKPIRTG
ISPLALIKGMTRPLSTLISSSQSCQYTLDAKRKHVAEAICKEQHLFLPFSYKNKYGMV
AQVTQTLKLEDTPKINSRFFGEGTKKMGLAFESTKSTSPPKQAEAVLKTLQELKKLTI
SEQNIQRANLFNKLVTELRGLSDEAVTSLLPQLIEVSSPITLQALVQCGQPQCSTHIL
QWLKRVHANPLLIDVVTYLVALIPEPSAQQLREIFNMARDQRSRATLYALSHAVNNYH
KTNPTGTQELLDIANYLMEQIQDDCTGDEDYTYLILRVIGNMGQTMEQLTPELKSSIL
KCVQSTKPSLMIQKAAIQALRKMEPKDKDQEVLLQTFLDDASPGDKRLAAYLMLMRSP
SQADINKIVQILPWEQNEQVKNFVASHIANILNSEELDIQDLKKLVKEALKESQLPTV
MDFRKFSRNYQLYKSVSLPSLDPASAKIEGNLIFDPNNYLPKESMLKTTLTAFGFASA
DLIEIGLEGKGFEPTLEALFGKQGFFPDSVNKALYWVNGQVPDGVSKVLVDHFGYTKD
DKHEQDMVNGIMLSVEKLIKDLKSKEVPEARAYLRILGEELGFASLHDLQLLGKLLLM
DRPDSLTIPAAQARKDACTFFRDGGLP"
BASE COUNT 894 a 758 c 706 g 778 t
ORIGIN
1 ccccaccgca gctggcgatg gacccgccga ggcccgcgct gctggcgctg cctgcgctgc
61 tgctgctgct gctggcgggc gccagggccg aagaggaaat gctggaaaat gtcagcctgg
121 tctgtccaaa agatgcgacc cgattcaagc acctccggaa gtacacatac aactatgagg
181 ctgagagttc cagtggagtc cctgggactg ctgattcaag aagtgccacc aggatcaact
241 gcaaggttga gctggaggtt ccccagctct gcagcttcat cctgaagacc agccagtgca
301 tcctgaaaga ggtgtatggc ttcaaccctg agggcaaagc cttgctgaag aaaaccaaga
361 actctgagga gtttgctgca gccatgtcca ggtatgagct caagctggcc attccagaag
421 ggaagcaggt tttcctttac ccggagaaag atgaacctac ttacatcctg aacatcaaga
481 ggggcatcat ttctgccctc ctggttcccc cagagacaga agaagccaag caagtgttgt
541 ttctggatac cgtgtatgga aactgctcca ctcactttac cgtcaagacg aggaagggca
601 atgtggcaac agaaatatcc actgaaagag acctggggca gtgtgatcgc ttcaagccca
661 tccgcacagg catcagccca cttgctctca tcaaaggcat gacccgcccc ttgtcaactc
721 tgatcagcag cagccagtcc tgtcagtaca cactggacgc taagaggaag catgtggcag
781 aagccatctg caaggagcaa cacctcttcc tgcctttctc ctacaagaat aagtatggga
841 tggtagcaca agtgacacag actttgaaac ttgaagacac accaaagatc aacagccgct
901 tctttggtga aggtactaag aagatgggcc tcgcatttga gagcaccaaa tccacatcac
961 ctccaaagca ggccgaagct gttttgaaga ctctccagga actgaaaaaa ctaaccatct
1021 ctgagcaaaa tatccagaga gctaatctct tcaataagct ggttactgag ctgagaggcc
1081 tcagtgatga agcagtcaca tctctcttgc cacagctgat tgaggtgtcc agccccatca
1141 ctttacaagc cttggttcag tgtggacagc ctcagtgctc cactcacatc ctccagtggc
1201 tgaaacgtgt gcatgccaac ccccttctga tagatgtggt cacctacctg gtggccctga
1261 tccccgagcc ctcagcacag cagctgcgag agatcttcaa catggcgagg gatcagcgca
1321 gccgagccac cttgtatgcg ctgagccacg cggtcaacaa ctatcataag acaaacccta
1381 cagggaccca ggagctgctg gacattgcta attacctgat ggaacagatt caagatgact
1441 gcactgggga tgaagattac acctatttga ttctgcgggt cattggaaat atgggccaaa
1501 ccatggagca gttaactcca gaactcaagt cttcaatcct gaaatgtgtc caaagtacaa
1561 agccatcact gatgatccag aaagctgcca tccaggctct gcggaaaatg gagcctaaag
1621 acaaggacca ggaggttctt cttcagactt tccttgatga tgcttctccg ggagataagc
1681 gactggctgc ctatcttatg ttgatgagga gtccttcaca ggcagatatt aacaaaattg
1741 tccaaattct accatgggaa cagaatgagc aagtgaagaa ctttgtggct tcccatattg
1801 ccaatatctt gaactcagaa gaattggata tccaagatct gaaaaagtta gtgaaagaag
1861 ctctgaaaga atctcaactt ccaactgtca tggacttcag aaaattctct cggaactatc
1921 aactctacaa atctgtttct cttccatcac ttgacccagc ctcagccaaa atagaaggga
1981 atcttatatt tgatccaaat aactaccttc ctaaagaaag catgctgaaa actaccctca
2041 ctgcctttgg atttgcttca gctgacctca tcgagattgg cttggaagga aaaggctttg
2101 agccaacatt ggaagctctt tttgggaagc aaggattttt cccagacagt gtcaacaaag
2161 ctttgtactg ggttaatggt caagttcctg atggtgtctc taaggtctta gtggaccact
2221 ttggctatac caaagatgat aaacatgagc aggatatggt aaatggaata atgctcagtg
2281 ttgagaagct gattaaagat ttgaaatcca aagaagtccc ggaagccaga gcctacctcc
2341 gcatcttggg agaggagctt ggttttgcca gtctccatga cctccagctc ctgggaaagc
2401 tgcttctgat ggatcgccca gactccctta cgatcccagc tgcacaagcc cgaaaagatg
2461 cttgtacttt cttcagagat ggaggtttgc cttgaatttc actgaagatg actcttggat
2521 cacatggaaa tgttaacatt tagaaattaa gctattcata atgttagctg tatttttaag
2581 agcattaatt tattcatctg gaaaacaatg ttcggtatac cttcctctac ctttgctgaa
2641 ggtcctttta tttttatttt tattttttta attttttgag atggagtctt gctcccaggc
2701 tggagtgcag tgatacaatc tcggctcact gcaactctgc cttccgggtt caagcaattc
2761 tcctgcctca gcctcccaag tagctgggac tgtggacgtg caccagcatg cccggctaat
2821 ttgtgtatct ttagtagaga caagcctgtt gacaaccatg tcaggctggt ttcgaactcc
2881 tgacctcaag tgatcctcca gcctgggcct cccacagtgc tggaataaca ggtgtgagcc
2941 actgcacctg acctgaaggt ccttttaaga ttgaaatgat acaatgatta taaaagaaag
3001 tatttggcaa actataattc actatctaaa tatgctataa tttttattat taattcataa
3061 aaggaaatat ataaatgtac tcctatggct tgattaaaaa aatgttgact ttaagaaaaa
3121 aaaaaaaaaa aaaaaa
//