LOCUS BC050449 2650 bp mRNA linear HUM 28-JUL-2005
DEFINITION Homo sapiens ADP-ribosylation factor guanine nucleotide-exchange
factor 2 (brefeldin A-inhibited), mRNA (cDNA clone IMAGE:6187759),
partial cds.
ACCESSION BC050449
VERSION BC050449.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2650)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2650)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-APR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 98 Row: l Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5453572.
FEATURES Location/Qualifiers
source 1..2650
/db_xref="H-InvDB:HIT000099200"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6187759"
/tissue_type="Peripheral Nervous System, sympathetic
trunk"
/clone_lib="Lupski_sympathetic_trunk"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..>2650
/gene="ARFGEF2"
/gene_synonym="BIG2"
/gene_synonym="dJ1164I10.1"
/db_xref="GeneID:10564"
/db_xref="MIM:605371"
CDS 153..>2650
/gene="ARFGEF2"
/gene_synonym="BIG2"
/gene_synonym="dJ1164I10.1"
/codon_start=1
/product="ARFGEF2 protein"
/protein_id="AAH50449.1"
/db_xref="GeneID:10564"
/db_xref="MIM:605371"
/translation="MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIK
AEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITG
NAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTIL
QTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQS
PVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGS
SLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDEN
SQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEG
PPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPD
VFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICA
DAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLEC
LVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSS
GTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFL
HQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQ
KIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYI
KMNRGINDSKDLPEEYLSSIYEEIEGKKIAKKKK"
BASE COUNT 748 a 651 c 679 g 572 t
ORIGIN
1 ggcggcgccg tggggccgag gtgtcgcttc ctgacggggc ggcgcggacg gacgcggccg
61 gtgccggccg ggacgccggg cccgcagcct agctcgccat ctcgctcacg ccgcccgccc
121 gcggggccgt cagcccccgc cgggccgggg ccatgcagga gagccagacc aagagcatgt
181 tcgtgtcccg ggccctggag aagatcctag ccgacaagga ggtgaagcgg ccccagcact
241 cccagctgcg cagggcctgc caggtggcgc tcgatgaaat taaagcagaa atagaaaagc
301 agaggcttgg cactgctgca ccaccaaagg caaacttcat tgaagctgac aagtattttc
361 ttccattcga gctagcttgc cagtccaagt ccccaagggt agtcagcaca tcccttgact
421 gcttgcagaa actcatcgca tacgggcaca tcactggcaa cgcccctgac agtggagccc
481 ctgggaagcg gctgatcgac agaattgttg aaaccatttg cagttgtttt cagggccctc
541 agactgatga aggggttcag ttacaaataa ttaaggctct tctgactgca gtgacttccc
601 cacacattga aattcatgag ggtactatcc tgcagacagt gagaacatgt tacaatatct
661 atttggccag caaaaatctc atcaatcaaa ccactgccaa ggctaccctt actcagatgc
721 tgaacgtcat tttcacccgc atggaaaacc aagtgttgca ggaggccaga gaactggaaa
781 aaccaatcca gtcaaaaccc cagtcccctg tgatccaagc tgcagcagta tccccaaagt
841 tcgttcgttt gaagcacagt caggcacaaa gcaaaccaac aactcccgaa aaaacagatt
901 taaccaacgg tgaacatgcc aggagtgatt ctggaaaagt aagcacagaa aatggagacg
961 cacccagaga aagaggctca tcactgtcag ggactgatga cggagcccag gaggtggtga
1021 aggacatctt ggaagatgta gtcacatctg ccattaaaga agcagcggaa aagcatggtc
1081 tgacagaacc tgagagagtt ctaggtgaac tggagtgcca ggaatgtgct attcccccag
1141 gagttgatga aaactcacag accaacggga tagccgatga caggcagtcc ttgtcgtcag
1201 cagataatct ggaatcggat gcacaaggac atcaagtggc tgccaggttc tcccacgttc
1261 tgcagaagga tgccttcctt gtgttccgct ccctgtgcaa gctgtccatg aaaccccttg
1321 gtgaaggccc tccagaccca aaatcccatg agctgcgttc caaggtggtt tccctgcagc
1381 tgctcctctc tgtgttgcaa aatgctggcc ccgtattcag gactcacgag atgttcatca
1441 atgcaatcaa gcaatatctc tgtgtggcct tgtccaaaaa cggcgtctct tcagtgcctg
1501 atgtctttga gctctctctt gccatttttc ttactcttct ttcaaacttt aaaatgcact
1561 tgaaaatgca gatagaggtc tttttcaaag agattttcct gaacatttta gaaacatcaa
1621 caagttcttt tgagcacagg tggatggtca ttcagactct gacgaggatc tgtgcagatg
1681 cccagtgtgt tgtggatatt tatgtcaact acgactgtga tttaaatgct gctaacattt
1741 ttgagcgcct tgtaaatgat ttatccaaaa ttgctcaggg aagaagtgga catgagctgg
1801 gaatgacacc tctgcaggag ctcagcctga ggaagaaagg cctggagtgc ctcgtgtcca
1861 ttctcaagtg catggtggag tggagcaaag acctgtatgt gaatcccaac caccagacca
1921 gcctcggtca ggagaggctc acggatcagg aaatagggga tgggaaaggc cttgacatgg
1981 caagacggtg tagtgtgacg tccatggagt ccacagtgtc ctcggggacc cagacaactg
2041 ttcaggatga ccctgagcaa tttgaggtca tcaagcaaca aaaagaaatc attgaacacg
2101 gcatcgagct gttcaacaag aaacccaaga gggggatcca gtttctccag gagcagggca
2161 tgctgggaac gtcagttgaa gacatagccc aattcctgca ccaggaggag cgcctggatt
2221 ccacccaagt aggcgatttt ctgggagata gcgcaaggtt caacaaggag gtgatgtatg
2281 cctacgtgga ccaacttgac ttctgtgaaa aagaatttgt ctcagccctg cggacattcc
2341 tagaaggttt ccgcctacct ggagaagccc aaaagattga ccgattaatg gagaagtttg
2401 ccgcaagata catagaatgc aaccaagggc aaactctgtt tgctagtgct gacactgctt
2461 atgtcctagc gtattcaatt attatgctga ctacagactt gcacagtcct caggtaaaaa
2521 ataaaatgac gaaagagcag tatattaaaa tgaatcgggg tatcaatgat agtaaagatc
2581 tgccagaaga gtatctctca agcatctatg aagagataga aggcaagaaa attgcaaaaa
2641 aaaaaaaaaa
//