LOCUS BC050221 1582 bp mRNA linear ROD 06-MAR-2007
DEFINITION Mus musculus left right determination factor 1, mRNA (cDNA clone
MGC:60608 IMAGE:30005856), complete cds.
ACCESSION BC050221
VERSION BC050221.1
KEYWORDS MGC.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 1582)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1582)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-APR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Kenneth R. Boheler (National Insitute on
Aging, USA)
cDNA Library Preparation: Yulan Piao and Minoru Ko (National
Institute on Aging, NIH: http://lgsun.grc.nia.nih.gov/cDNA/)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 111 Row: d Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 46559379.
FEATURES Location/Qualifiers
source 1..1582
/organism="Mus musculus"
/mol_type="mRNA"
/strain="129/Sv x 129/Sv-CP"
/db_xref="taxon:10090"
/clone="MGC:60608 IMAGE:30005856"
/tissue_type="ES Cell, undifferentiated, mouse, R1
Embryonic stem cells"
/clone_lib="NIA Mouse Undifferentiated ES Cell cDNA
Library (Long)"
/lab_host="DH10B"
/note="Vector: pSPORT1"
gene 1..1582
/gene="Lefty1"
/gene_synonym="lefty"
/gene_synonym="lefty-1"
/db_xref="GeneID:13590"
/db_xref="MGI:MGI:107405"
CDS 35..1141
/gene="Lefty1"
/gene_synonym="lefty"
/gene_synonym="lefty-1"
/codon_start=1
/product="left right determination factor 1"
/protein_id="AAH50221.1"
/db_xref="GeneID:13590"
/db_xref="MGI:MGI:107405"
/translation="MPFLWLCWALWALSLVSLREALTGEQILGSLLQQLQLDQPPVLD
KADVEGMVIPSHVRTQYVALLQHSHASRSRGKRFSQNLREVAGRFLVSETSTHLLVFG
MEQRLPPNSELVQAVLRLFQEPVPRTALRRQKRLSPHSARARVTIEWLRFRDDGSNRT
ALIDSRLVSIHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPGTWSSHKL
VRFAAQGTPDGKGQGEPQLELHTLDLKDYGAQGNCDPEAPVTEGTRCCRQEMYLDLQG
MKWAENWILEPPGFLTYECVGSCLQLPESLTSRWPFLGPRQCVASEMTSLPMIVSVKE
GGRTRPQVVSLPNMRVQTCSCASDGALIPRRLQP"
BASE COUNT 337 a 450 c 469 g 326 t
ORIGIN
1 cacacatcca aggctcctct tcccggacag caccatgcca ttcctgtggc tctgctgggc
61 actctgggca ctgtcgctgg ttagcctcag ggaagccctg accggagagc agatcctggg
121 cagcctgctg caacagctgc agctcgatca accgccagtc ctggacaagg ctgatgtgga
181 agggatggtc atcccctcgc acgtgaggac tcagtatgtg gccctgctac aacacagcca
241 tgccagccgc tcccgaggca agaggttcag ccagaacctt cgagaggtgg caggcaggtt
301 cctggtgtca gagacctcca ctcacctgct agtgttcgga atggagcagc ggctgccgcc
361 taacagcgag ctggtgcagg ctgtgctgcg gctgttccag gagcctgtgc ccagaacagc
421 tctccggagg caaaagaggc tgtccccaca cagtgcccgg gctcgggtca ccattgaatg
481 gctgcgcttc cgcgacgacg gctccaaccg cactgccctt atcgattcta ggctcgtgtc
541 catccacgag agcggctgga aggccttcga cgtgaccgag gccgtgaact tctggcagca
601 gctgagccgg ccgaggcagc cgctgctgct ccaggtgtcg gtgcagaggg agcatctggg
661 gccgggaacc tggagctcac acaagttggt tcgtttcgcg gcgcagggga cgccggatgg
721 caaggggcag ggcgagccac agctggagct gcacacgctg gacctcaagg actatggagc
781 tcaaggcaat tgtgaccccg aggcaccagt gactgaaggc acccgatgct gtcgccagga
841 gatgtacctg gacctgcagg ggatgaagtg ggccgagaac tggatcctag aaccgccagg
901 gttcctgaca tatgaatgtg tgggcagctg cctgcagcta ccggagtccc tgaccagcag
961 gtggccattt ctggggcctc ggcagtgtgt cgcctcagag atgacctccc tgcccatgat
1021 tgtcagcgtg aaggagggag gcaggaccag gcctcaagtg gtcagcctgc ccaacatgag
1081 ggtgcagacc tgtagctgcg cctcagatgg ggcgctcata cccaggaggc tgcagccata
1141 ggcgcggggt gtggcttccc caaggatgtg cctttcatgc aaatctgaag tgctcattat
1201 actgggagag ctggggattc taactcccta atgggcaatc cctgtgtgtg ctctttgctt
1261 cctctgaagt agcctcatcc ctaaattttt accttcgagg aatgtgactc gctggcccct
1321 ggaggcgctc tgacccagtg gtctctgtcc ttcatattgt tcactgcact gtatgcgaag
1381 cacttacatg tatagatact gcaaaccaag gacagaatcc ccaattgcca ttgttccctt
1441 aatttgtcgc tgaatctggg ctgagtccca gtcttgactc tggacctaag ccacaagttg
1501 ggcaaacatg tccaacctag gcaatactgg ctttgctaga tgtgaataaa atatgctttg
1561 ttttgtaaaa aaaaaaaaaa aa
//