LOCUS BC049414 1532 bp mRNA linear VRT 15-JUL-2006
DEFINITION Danio rerio zgc:56376, mRNA (cDNA clone MGC:56376 IMAGE:5604582),
complete cds.
ACCESSION BC049414
VERSION BC049414.1
KEYWORDS MGC.
SOURCE Danio rerio (zebrafish)
ORGANISM Danio rerio
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE 1 (bases 1 to 1532)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1532)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Sumio Sugano
cDNA Library Preparation: Dr. Sumio Sugano
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 104 Row: a Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 29436439.
FEATURES Location/Qualifiers
source 1..1532
/organism="Danio rerio"
/mol_type="mRNA"
/db_xref="taxon:7955"
/clone="MGC:56376 IMAGE:5604582"
/tissue_type="Whole body, adult male"
/clone_lib="Sugano SJD adult male"
/lab_host="DH10B"
/note="Vector: pME18S-FL3"
gene 1..1532
/gene="zgc:56376"
/gene_synonym="MGC56376"
/gene_synonym="Rnf30"
/db_xref="GeneID:394070"
/db_xref="ZFIN:ZDB-GENE-040426-1003"
CDS 122..1168
/gene="zgc:56376"
/gene_synonym="MGC56376"
/gene_synonym="Rnf30"
/codon_start=1
/product="zgc:56376"
/protein_id="AAH49414.1"
/db_xref="GeneID:394070"
/db_xref="ZFIN:ZDB-GENE-040426-1003"
/translation="MDIQRTASLLGTPDSMEGLEKQLSCPICLDMFTKPVVILPCQHN
LCRGCASDLYDSRNPYRFSGGVFRCPTCRFEVVLDRHGVHGLQRNLLVENIIDIYKQQ
QEGGGGGGGSTTESPIKPKSSKELMCQEHEDEKINIYCVTHQTPTCSMCKVFGQHKDC
EVSPLASVYQAQKGELSNAIDALVAGNGRLQALLNQMEEACTAVQDNAQRAKQKFGEC
FDSLYAALEERKNALLERIGKEQDEKVGALRSLARRYGDRLQAATELTDMAVQALEQS
GAAEFLQASKNLISQTKDAAKSSLAEERPEPGFERMDHFTLDTEQVETLLSKMDFGGE
DDEEFEDAEDQEEE"
BASE COUNT 450 a 299 c 372 g 411 t
ORIGIN
1 atagatctga caactgcagg gaatcattta ctgctgtgtg cagcagcttg tttttaacct
61 catttttcaa ggagaacgcc actaaagtga tacagcaaaa ggagccagag caaccaccaa
121 catggacatt cagcgaactg cctctttgtt ggggactcct gactcgatgg agggccttga
181 gaaacagcta agctgcccaa tatgtctgga catgtttacc aaaccagtgg tcatcctgcc
241 gtgtcagcat aacctgtgtc gaggatgtgc tagtgatctt tatgactctc gaaaccctta
301 tcgtttctct ggtggtgttt ttcggtgccc aacttgtcga tttgaagtgg ttcttgatcg
361 ccatggtgtg catggacttc agaggaatct tcttgttgag aacataatcg acatttacaa
421 acagcaacaa gagggtggtg gaggaggtgg tggcagcacc actgaaagtc caataaaacc
481 caaaagctcc aaagagctaa tgtgtcaaga acacgaggac gagaagatca acatctactg
541 cgttactcac caaactccca cttgttccat gtgtaaggta tttggccagc ataaggactg
601 tgaggtgtca ccacttgcta gtgtctatca ggctcaaaag ggagagctga gcaatgccat
661 tgatgctctt gtggcgggga atggccgcct tcaggcgttg cttaaccaga tggaggaggc
721 ctgcacagct gttcaggaca atgctcaacg tgccaaacaa aagtttggcg aatgcttcga
781 ttccctctat gcagccttgg aggagcgtaa aaatgccctc ttagagcgca ttggcaaaga
841 gcaagacgaa aaggttggtg cactcaggag tttggctagg cgttatgggg accgtcttca
901 ggcagccaca gagttaacag acatggcggt tcaggctctg gagcaaagtg gtgccgcgga
961 gttcctgcag gcctccaaga acctgatctc ccagacaaaa gacgcagcaa agagctcact
1021 tgctgaagaa aggcccgagc caggctttga aagaatggac catttcactt tggatacgga
1081 gcaggtggag actttgttgt ccaagatgga ctttggagga gaggatgatg aggaatttga
1141 agacgctgaa gatcaagaag aggagtaaat aagttgcaag aaaagaagag gtttaggaag
1201 gtttagcaag gtcattgaca aatgagaaaa taaaagtttt aatattcgta tttcactgct
1261 aagcagtgtt gtcatttcaa catgtgtaat caaaagaata tttccaaata actaggtgcc
1321 attttttata ctttttataa tttagtcaaa atgctgctgt tgttcttaaa agtcatcttc
1381 ctacttttgt ttcgctcaga aatgagtcac tgttgtttat ttctgcagtc tgttgttact
1441 agttctgtat tttcagttta ctctgttttt caatgttctt tattgtttga aaataaataa
1501 aattaaaaat tgaaaaaaaa aaaaaaaaaa aa
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