LOCUS BC048797 3110 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens DNA fragmentation factor, 40kDa, beta polypeptide
(caspase-activated DNase), mRNA (cDNA clone MGC:51917
IMAGE:5204996), complete cds.
ACCESSION BC048797
VERSION BC048797.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3110)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3110)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (14-MAR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 93 Row: i Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 51988888.
FEATURES Location/Qualifiers
source 1..3110
/db_xref="H-InvDB:HIT000053337"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:51917 IMAGE:5204996"
/tissue_type="Lung, Spleen, fetal, pooled"
/clone_lib="NIH_MGC_122"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3110
/gene="DFFB"
/gene_synonym="CAD"
/gene_synonym="CPAN"
/gene_synonym="DFF-40"
/gene_synonym="DFF2"
/gene_synonym="DFF40"
/db_xref="GeneID:1677"
/db_xref="HGNC:HGNC:2773"
/db_xref="MIM:601883"
CDS 324..1340
/gene="DFFB"
/gene_synonym="CAD"
/gene_synonym="CPAN"
/gene_synonym="DFF-40"
/gene_synonym="DFF2"
/gene_synonym="DFF40"
/codon_start=1
/product="DNA fragmentation factor, 40kDa, beta
polypeptide (caspase-activated DNase)"
/protein_id="AAH48797.1"
/db_xref="GeneID:1677"
/db_xref="HGNC:HGNC:2773"
/db_xref="MIM:601883"
/translation="MLQKPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGS
RLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQGYVSDIRRFLSAFHEPQVGLIQA
AQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSC
ESRIRSYLREVSSYPSTVGAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLC
TPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNLDHIIEKKRTIIPTLVEAI
KEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTHKLNCDPSRIYKPQTRLKRKQPV
RKRQ"
BASE COUNT 747 a 760 c 816 g 787 t
ORIGIN
1 ccgctactct ggccctcagg gttgtgtagt ttccggggcc acggcacctg ggcacaccag
61 gtggcgtcgc gcctttgctt tcctgagcct tctgagtaag gtaatgtggt gtccgtgggg
121 cgacgcctgc gcacaagacc gcgtcgggcc tcacttttcc caggccttct gggtaatgta
181 gtttccggga tcggcacccg gcctgtgcca gcttgcagag ctcaccaggt gcagacccct
241 gcggccaggg cgaggacgga tctgagcagc tgggcagcag gtgccaccgc ctgtgggacc
301 cagagggctt gaggacatct gcaatgctcc agaagcccaa gagcgtgaag ctgcgggccc
361 tgcgcagccc gaggaagttc ggcgtggctg gccggagctg ccaggaggtg ctgcgcaagg
421 gctgtctccg cttccagctc cctgagcgcg gttcccggct gtgcctgtac gaggatggca
481 cggagctgac ggaagattac ttccccagtg ttcccgacaa cgccgagctg gtgctgctca
541 ccttgggcca ggcctggcag ggctatgtga gcgacatcag gcgcttcctc agtgcatttc
601 acgagccaca ggtggggctc atccaggccg cccagcagct gctgtgtgat gagcaggccc
661 cacagaggca gaggctgctg gctgacctcc tgcacaacgt cagccagaac atcgcggccg
721 agacccgggc tgaggacccg ccgtggtttg aaggcttgga gtcccgattt cagagcaagt
781 ctggctatct gagatacagc tgtgagagcc ggatccggag ttacctgagg gaggtgagct
841 cctacccctc cacagtgggt gcggaggctc aggaggaatt cctgcgggtc ctcggctcca
901 tgtgccagag gctccggtcc atgcagtaca atggcagcta cttcgacaga ggagccaagg
961 gcggcagccg cctctgcaca ccggaaggct ggttctcctg ccagggtccc tttgacatgg
1021 acagctgctt atcaagacac tccatcaacc cctacagtaa cagggagagc aggatcctct
1081 tcagcacctg gaacctggat cacataatag aaaagaaacg caccatcatt cctacactgg
1141 tggaagcaat taaggaacaa gatggaagag aagtggactg ggagtatttt tatggcctgc
1201 tttttacctc agagaaccta aaactagtgc acattgtctg ccataagaaa accacccaca
1261 agctcaactg tgacccgagc agaatctaca aaccccagac aaggttgaag cggaagcagc
1321 ctgtgcggaa acgccagtga cacgtacaca ccacgtcctg gtctttgttt gaggcctgac
1381 gtgggcatca ttttaacagg tgcctttttt gtttttttgt ttttcgtttt tttggtcact
1441 ccagtagctc ctggaaaaaa ccttaaaaaa tgtttcctcc aaatctgatt tcattacatt
1501 tctgaattgt tgggtttttt tttgttgttt tgttttgttt tgtagatgga gtttcacttt
1561 tgttgcccag gctggagtgt agtggcgcga tctcggctca gcctcccgag tagctgggat
1621 tacaggcatg tgccaccacg cccggctaat gtttgtattt ttagtagaga cggggtttca
1681 ccatgttggt caggctggtc tcaaactcct gacctcaggt gatccgccca cctcagcctc
1741 ccaaagtgct gggatgacag gtgtgagcca ctgcgcccag cctgaatcat ttcttatacc
1801 ttctgacagc ccaacttcca gaggacagct ctggggtact cgttggatgt ctgtgagtac
1861 ctggtcatac gggtcagtag ggataagaat tgtctctggg ctgaggaatt cttctgttct
1921 ctggtttcac cagcgttggg tttgctcatg taatgtggtc accatactca aatggtgtca
1981 tggctgaagt tggccacctt gcttgaggga caagttgttt atgtatcagc tctctgctgg
2041 gtctcccttt ccatggcaaa tgggcagctc catcctcttg actcttctaa atgcccaaaa
2101 gaggtgtcat gctttggggg tacgatgttt atactccgta aagaacatac aaggacattc
2161 actgctgatt tttttttttg tttgtttgag acagggtctc actctgtcgc tcaggctgga
2221 gtgcagtgat gcaatcttgg ctcactgcaa cctccgcctc tcaggttcaa gtggttctcc
2281 tgcctcagcc tcccaagtag ctgggattac aggcacctac caccagggcc agctaatttt
2341 tgtatgttta gtagtaacgg ggtttcacca tgttggccag gctgttctcg aactcctgac
2401 ctcaggtgat ctgcccgcct cggtctccca aagtgctggg attacaggca tgagccactg
2461 cacctgacct gctgaattgt ttataatggc aagaaatagg aaacccccca atgtctgttg
2521 aacagctatc acgttgaacc acgtgaaact gctgttttct aggccaaaaa tggtgagcga
2581 tcatttattt catgattcaa cctgatacat ttacatagtg caaaactgtg tcacagtttc
2641 aggcttttat gaggaaagcg tttctgtgta gaaactggaa gctgttcagg gcatcggcag
2701 ctgaaccctg ctccgttggt cagcgttact atcatctcgg atcatatgga gctcatgtca
2761 gccgtgtggg tggcgggtgc acagagacgg tctggaagga aacacgcgga tctgaacagc
2821 agtaatcctg ggggatacgg gggttgggct agattacaga gggctcattt tctacgtcat
2881 gtattttatg atacttgaat tttttgaaat gggcatttat tttataacat gttaaaatgt
2941 actttttaaa ttaagtcatt ttgtaatatt tgaattttta catttgttgt acaatcagga
3001 aaagcaataa agatttttca aaaataaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3061 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//