LOCUS BC048259 3652 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphatidylinositol binding clathrin assembly
protein, mRNA (cDNA clone MGC:51891 IMAGE:5537605), complete cds.
ACCESSION BC048259
VERSION BC048259.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3652)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3652)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-MAR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 93 Row: o Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 56788365.
FEATURES Location/Qualifiers
source 1..3652
/db_xref="H-InvDB:HIT000098930"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:51891 IMAGE:5537605"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3652
/gene="PICALM"
/gene_synonym="CALM"
/gene_synonym="CLTH"
/gene_synonym="LAP"
/db_xref="GeneID:8301"
/db_xref="HGNC:HGNC:15514"
/db_xref="MIM:603025"
CDS 79..2037
/gene="PICALM"
/gene_synonym="CALM"
/gene_synonym="CLTH"
/gene_synonym="LAP"
/codon_start=1
/product="phosphatidylinositol binding clathrin assembly
protein"
/protein_id="AAH48259.2"
/db_xref="GeneID:8301"
/db_xref="HGNC:HGNC:15514"
/db_xref="MIM:603025"
/translation="MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDY
LIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNT
LFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMN
TEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINL
LEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLD
ALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQAR
LKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPND
LLDLQQPTFHPSVHPMSTASQVASTWGDPFSATVDAVDDAIPSLNPFLTKSSGDVHLS
ISSDVSTFTTRTPTHEMFVGFTPSPVAQPHPSAGLNVDFESVFGNKSTNVIVDSGGFD
ELGGLLKPTVASQNQNLPVAKLPPSKLVSDDLDSSLANLVGNLGIGNGTTKNDVNWSQ
PGEKKLTGGSNWQPKVAPTTAWNAATMAPPVMAYPATTPTGMIGYGIPPQMGSVPVMT
QPTLIYSQPVMRPPNPFGPVSGAQIQFM"
BASE COUNT 1116 a 708 c 715 g 1113 t
ORIGIN
1 ggtggggacc ctccggctct tgggggtccc agtccccgcc ggctgctgag cgggtggggt
61 ggtggaggag ctgcagagat gtccggccag agcctgacgg accgaatcac tgccgcccag
121 cacagtgtca ccggctctgc cgtatccaag acagtatgca aggccacgac ccacgagatc
181 atggggccca agaaaaagca cctggactac ttaattcagt gcacaaatga gatgaatgtg
241 aacatcccac agttggcaga cagtttattt gaaagaacta ctaatagtag ttgggtggtg
301 gtcttcaaat ctctcattac aactcatcat ttgatggtgt atggaaatga gcgttttatt
361 cagtatttgg cttcaagaaa cacgttgttt aacttaagca attttttgga taaaagtgga
421 ttgcaaggat atgacatgtc tacatttatt aggcggtata gtagatattt aaatgagaaa
481 gcagtttcat acagacaagt tgcatttgat ttcacaaaag tgaagagagg ggctgatgga
541 gttatgagaa caatgaacac agaaaaactc ctaaaaactg taccaattat tcagaatcag
601 atggatgcac ttcttgattt taatgttaat agcaatgaac ttacaaatgg ggtaataaat
661 gctgccttca tgctcctgtt caaagatgcc attagactgt ttgcagcata caatgaagga
721 attattaatt tgttggaaaa atattttgat atgaaaaaga accaatgcaa agaaggtctt
781 gacatctata agaagttcct aactaggatg acaagaatct cagagttcct caaagttgca
841 gagcaagttg gaattgacag aggtgatata ccagaccttt cacaggcccc tagcagtctt
901 cttgatgctt tggaacaaca tttagcttcc ttggaaggaa agaaaatcaa agattctaca
961 gctgcaagca gggcaactac actttccaat gcagtgtctt ccctggcaag cactggtcta
1021 tctctgacca aagtggatga aagggaaaag caggcagcat tagaggaaga acaggcacgt
1081 ttgaaagctt taaaggaaca gcgcctaaaa gaacttgcaa agaaacctca tacctcttta
1141 acaactgcag cctctcctgt atccacctca gcaggaggga taatgactgc accagccatt
1201 gacatatttt ctacccctag ttcttctaac agcacatcaa agctgcccaa tgatctgctt
1261 gatttgcagc agccaacttt tcacccatct gtacatccta tgtcaactgc ttctcaggta
1321 gcaagtacat ggggagatcc tttctctgct actgtagatg ctgttgatga tgccattcca
1381 agcttaaatc ctttcctcac aaaaagtagt ggtgatgttc acctttccat ttcttcagat
1441 gtatctactt ttactactag gacacctact catgaaatgt ttgttggatt cactccttct
1501 ccagttgcac agccacaccc ttcagctggc cttaatgttg actttgaatc tgtgtttgga
1561 aataaatcta caaatgttat tgtagattct gggggctttg atgaactagg tggacttctc
1621 aaaccaacag tggcctctca gaaccagaac cttcctgttg ccaaactccc acctagcaag
1681 ttagtatctg atgacttgga ttcatcttta gccaaccttg tgggcaatct tggcatcgga
1741 aatggaacca ctaagaatga tgtaaattgg agtcaaccag gtgaaaagaa gttaactggg
1801 ggatctaact ggcaaccaaa ggttgcacca acaaccgctt ggaatgctgc aacaatggca
1861 ccccctgtaa tggcctatcc tgctactaca ccaacaggca tgataggata tggaattcct
1921 ccacaaatgg gaagtgttcc tgtaatgacg caaccaacct taatatacag ccagcctgtc
1981 atgagacctc caaacccctt tggccctgta tcaggagcac agatacagtt tatgtaactt
2041 gatggaagaa aatggaatta ctccaaaaag acaagtgctc aagcagcaaa atccttactt
2101 ccagcaaaat ccaaactgct gtctcttaaa tctcttaaac tctcttcttc cattagaatg
2161 ctacaagtaa ctcagtgaag gcccatgaag gaaattggga ctagtttata ggagaacgta
2221 tcaatacagt ttataaagcc aagaattgct atgatttaag actaagatct gtctttttgg
2281 tgactaaccc ttcaattctt tcaactcctg ttaataccca taatcagtaa cctatcaaga
2341 aaagccctta tttggaaagt gtgaaatttg tatttggaaa agctgcctgg agagaagaac
2401 tgtgtccttt actgtatttc aacaggactc ttttggggga tcaaaattaa aattcctaat
2461 tatgcattat ctttcttttc tccagtcctc acaaatacag aaacaataac tgaaattaac
2521 ttttcttttt ttaaaaaaaa ttatattcag tttgcagtag acattcctta agtatttgta
2581 tttatttatg attatcaatt ttacataaca ttaatattgt atcagacctc cttatgaaaa
2641 tgagtatgga tgtgcacagt atgtttgatt tttatccaca agaatgaatc tgattcagaa
2701 tgcttttctc agctgacata cagagcacta aatattttaa ggcaagtcca taggtctgaa
2761 tctcttaaga attctcggcc tctgtgggat ttagggaagc attataaatg cattaatcct
2821 tatagtcaat tctgtgccta ggattttgcc agggaacagt tcactgacta ggaaaagcac
2881 tacattttaa attcagcatt agtgcattgg gaaggatctt tactgctttg tgcttggcat
2941 gtcattattt tccatttgac attagggcct ttccaaaatg aatgtgagga attgctttca
3001 cttcaagact ttccttcttt tcactaaaac tctagaaggt gttacaaggg ggagggaagg
3061 ggggcaaagt ccttgaacat tttctttggc tcgtgccatg ttatgatcat atacctttta
3121 aataagggga aatagtatct ttaaagttaa tgtctagcca agagtttagt aaacgaagaa
3181 ttaaactgca ctgttgatcg gtgctttgtg taaatacatc tttaacattt gggtggagag
3241 gggccttaag aaggacagtt cattgtagga aagcaattct gtacatgagt ttaagcattc
3301 ttgttgcatt gtctctgcag attctatttt tgtttacaat attaaaatgt atgttagcaa
3361 aatgggtgga ttttcaaata aaatgcagct tccacaaaag ttttgttatg gtattctggt
3421 ctgagatgca ttttcatttt tcctttctct ttttattatc aatattgtca tttttcccta
3481 ataaaatata cccaggtgat tatatttgtt gatctaataa catggaaggt ttgttttata
3541 tgaattttca aaaagatgtc tctttacact ttttgttacc ttgtagactc ttattgataa
3601 atgcaactac ttattaaaat tgttcacttt ttgtcttttg aaaaaaaaaa aa
//