LOCUS BC047621 3041 bp mRNA linear HUM 28-JUL-2005
DEFINITION Homo sapiens nicastrin, mRNA (cDNA clone MGC:51016 IMAGE:4827966),
complete cds.
ACCESSION BC047621
VERSION BC047621.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3041)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3041)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-MAR-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 90 Row: i Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 24638432.
FEATURES Location/Qualifiers
source 1..3041
/db_xref="H-InvDB:HIT000053185"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:51016 IMAGE:4827966"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3041
/gene="NCSTN"
/gene_synonym="APH2"
/gene_synonym="KIAA0253"
/db_xref="GeneID:23385"
/db_xref="MIM:605254"
CDS 281..2350
/gene="NCSTN"
/gene_synonym="APH2"
/gene_synonym="KIAA0253"
/codon_start=1
/product="NCSTN protein"
/protein_id="AAH47621.1"
/db_xref="GeneID:23385"
/db_xref="MIM:605254"
/translation="MDFNLILESLCRGNSVERKIYIPLNKTAPCVRLLNATHQIGCQS
SISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLMEKLKGRTSRIAGLA
VSLTKPSPASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQWNSLGNGLAYEDFSFPI
FLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHAVISTATCMRRSSIQS
TFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLDSRSFFWNVAPGAE
SAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMEKGKFPV
QLENVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVI
LRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEW
LSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIK
ANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQC
QDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTE
YSTWTESRWKDIHARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPR
EPGAVSY"
BASE COUNT 729 a 808 c 707 g 797 t
ORIGIN
1 agagacggtg tcagtggtag cctagagagg ccgctaacag acaggagccg aacgggggct
61 tccgctcagc agagaggcaa gatggctacg gcagggggtg gctctggggc tgacccggga
121 agtcggggtc tccttcgcct tctgtctttc tgcgtcctac tagcagtgtc actgtcaatg
181 gcgctacatg gactttgtaa taaccctttg aggcacatag ctgggtgcca tgtagaacat
241 gtatctgtta cgataagtgt gtgcccaaga aatcagaaga atggacttta atctcatttt
301 agaaagtttg tgcaggggaa actcagtgga gaggaagata tatatcccct taaataaaac
361 agctccctgt gttcgcctgc tcaacgccac tcatcagatt ggctgccagt cttcaattag
421 tggagacaca ggggttatcc acgtagtaga gaaagaggag gacctacagt gggtattgac
481 tgatggcccc aacccccctt acatggttct gctggagagc aagcatttta ccagggattt
541 aatggagaag ctgaaaggga gaaccagccg aattgctggt cttgcagtgt ccttgaccaa
601 gcccagtcct gcctcaggct tctctcctag tgtacagtgc ccaaatgatg ggtttggtgt
661 ttactccaat tcctatgggc cagagtttgc tcactgcaga gaaatacagt ggaattcgct
721 gggcaatggt ttggcttatg aagactttag tttccccatc tttcttcttg aagatgaaaa
781 tgaaaccaaa gtcatcaagc agtgctatca agatcacaac ctgagtcaga atggctcagc
841 accaaccttc ccactatgtg ccatgcagct cttttcacac atgcatgctg tcatcagcac
901 tgccacctgc atgcggcgca gctccatcca aagcaccttc agcatcaacc cagaaatcgt
961 ctgtgacccc ctgtctgatt acaatgtgtg gagcatgcta aagcctataa atacaactgg
1021 gacattaaag cctgacgaca gggttgtggt tgctgccacc cggctggata gtcgttcctt
1081 tttctggaat gtggccccag gggctgaaag cgcagtggct tcctttgtca cccagctggc
1141 tgctgctgaa gctttgcaaa aggcacctga tgtgaccacc ctgccccgca atgtcatgtt
1201 tgtcttcttt caaggggaaa cttttgacta cattggcagc tcgaggatgg tctacgatat
1261 ggagaagggc aagtttcccg tgcagttaga gaatgttgac tcatttgtgg agctgggaca
1321 ggtggcctta agaacttcat tagagctttg gatgcacaca gatcctgttt ctcagaaaaa
1381 tgagtctgta cggaaccagg tggaggatct cctggccaca ttggagaaga gtggtgctgg
1441 tgtccctgct gtcatcctca ggaggccaaa tcagtcccag cctctcccac catcttccct
1501 gcagcgattt cttcgagctc gaaacatctc tggcgttgtt ctggctgacc actctggtgc
1561 cttccataac aaatattacc agagtattta cgacactgct gagaacatta atgtgagcta
1621 tcccgaatgg ctgagccctg aagaggacct gaactttgta acagacactg ccaaggccct
1681 ggcagatgtg gccacggtgc tgggacgtgc tctgtatgag cttgcaggag gaaccaactt
1741 cagcgacaca gttcaggctg atccccaaac ggttacccgc ctgctctatg ggttcctgat
1801 taaagccaac aactcatggt tccagtctat cctcaggcag gacctaaggt cctacttggg
1861 tgacgggcct cttcaacatt acatcgctgt ctccagcccc accaacacca cttatgttgt
1921 acagtatgcc ttggcaaatt tgactggcac agtggtcaac ctcacccgag agcagtgcca
1981 ggatccaagt aaagtcccaa gtgaaaacaa ggatctgtat gagtactcat gggtccaggg
2041 ccctttgcat tctaatgaga cggaccgact cccccggtgt gtgcgttcta ctgcacgatt
2101 agccagggcc ttgtctcctg cctttgaact gagtcagtgg agctctactg aatactctac
2161 atggactgag agccgctgga aagatatcca tgcccggata tttctcatcg ccagcaaaga
2221 gcttgagttg atcaccctga cagtgggctt cggcatcctc atcttctccc tcatcgtcac
2281 ctactgcatc aatgccaaag ctgatgtcct tttcattgct ccccgggagc caggagctgt
2341 gtcatactga ggaggacccc agcttttctt gccagctcag cagttcactt cctagagcat
2401 ctgtcccact gggacacaac cactaatttg tcactggaac ctccctgggc ctgtctcaga
2461 ttgggattaa cataaaagag tggaactatc caaaagagac agggagaaat aaataaattg
2521 cctcccttcc tccgctcccc tttcccatca ccccttcccc atttcctctt ccttctctac
2581 tcatgccaga ttttgggatt acaaatagaa gcttcttgct cctgtttaac tccctagtta
2641 cccaccctaa tttgcccttc aggacccttc tactttttcc ttcctgccct gtacctctct
2701 ctgctcctca cccccacccc tgtacccagc caccttcctg actgggaagg acataaaagg
2761 tttaatgtca gggtcaaact acattgagcc cctgaggaca ggggcatctc tgggctgagc
2821 ctactgtctc cttcccactg tcctttctcc aggccctcag atggcacatt agggtgggcg
2881 tgctgcgggt gggtatccca cctccagccc acagtgctca gttgtacttt ttattaagct
2941 gtaatatcta tttttgtttt tgtctttttc ctttattctt tttgtaaata tatatataat
3001 gagtttcatt aaaatagatt atcccaaaaa aaaaaaaaaa a
//