LOCUS BC046149 2126 bp mRNA linear HUM 03-JAN-2005
DEFINITION Homo sapiens nitrilase 1, mRNA (cDNA clone MGC:57670
IMAGE:5764281), complete cds.
ACCESSION BC046149
VERSION BC046149.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2126)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2126)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 107 Row: j Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5031946.
FEATURES Location/Qualifiers
source 1..2126
/db_xref="H-InvDB:HIT000053002"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:57670 IMAGE:5764281"
/tissue_type="Brain, adult, 6 pooled whole brains"
/clone_lib="NIH_MGC_114"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2126
/gene="NIT1"
/db_xref="GeneID:4817"
/db_xref="MIM:604618"
CDS 60..791
/gene="NIT1"
/codon_start=1
/product="NIT1 protein"
/protein_id="AAH46149.1"
/db_xref="GeneID:4817"
/db_xref="MIM:604618"
/translation="MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSC
ELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGPCLAFLPEAFDFIARDPAETLHLS
EPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYR
KTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQA
GAEILTYPSAFGSITGPAHWEPVSS"
BASE COUNT 590 a 541 c 511 g 484 t
ORIGIN
1 cttctggctc cagaccgccc tccggatcgg accctgcgaa tggttttggc tatatcttca
61 tgctgggctt catcaccagg cctcctcaca gattcctgtc ccttctgtgt cctggactcc
121 ggatacctca actctcagta ctttgtgctc agcccaggcc cagagccatg gctatctcct
181 cttcctcctg cgaactgccc ctggtggctg tgtgccaggt aacatcgacg ccagacaagc
241 aacagaactt taaaacatgt gctgagctgg ttcgagaggc tgccagactg ggtccctgcc
301 tggctttcct gcctgaggca tttgacttca ttgcacggga ccctgcagag acgctacacc
361 tgtctgaacc actgggtggg aaacttttgg aagaatacac ccagcttgcc agggaatgtg
421 gactctggct gtccttgggt ggtttccatg agcgtggcca agactgggag cagactcaga
481 aaatctacaa ttgtcacgtg ctgctgaaca gcaaaggggc agtagtggcc acttacagga
541 agacacatct gtgtgacgta gagattccag ggcaggggcc tatgtgtgaa agcaactcta
601 ccatgcctgg gcccagtctt gagtcacctg tcagcacacc agcaggcaag attggtctag
661 ctgtctgcta tgacatgcgg ttccctgaac tctctctggc attggctcaa gctggagcag
721 agatacttac ctatccttca gcttttggat ccattacagg cccagcccac tgggagcccg
781 tctcctcttg agggtgacgt agggcagcag gtcgtggcga gtgatgatgc gcagcagctg
841 cagcacctgg cgaaagttac tttcatcaca gcggccctgg cgctccagtg ccaataagaa
901 gtcacgtcca tttcggatga gtccacgctc gtggtcatca atgacatcaa caaagaggaa
961 agaaagcacg cgcacatctc tgtgtgtcag atgagtgccc acgatgtcaa acatgcggtg
1021 caggctgtac agcccatgtt cctgctcacc atgctcttct ggccacacct ggcttgcccg
1081 ccgctttagg cccgccatgc tgggggctca ggtacgcaat gctttccaga atccctgctc
1141 agcagctgca atccccaccg tactgaaagg gcagggaaag aaccgatgag acactcaagg
1201 gcaggaacta gtctcatgca ttcccatatt cccagttgct aacaatgcct ggcacagtat
1261 agtgctttat acatgtttat taaatttttg aagtgtttgt ttaaaaataa acactggcca
1321 ggcacagggg ctcacacctg taatcccagc attttgggag gccaaggcag gaggatcact
1381 tgagcccagg cgtttaagac cagcctgggc aacataggga gacccgtttc tacaaaaaaa
1441 gaaaaaatta gctgggcttg gtggtacacg cctgtagtcc cagctgcaca ggaggctgag
1501 gtgggaggaa ggcttgaggc caggagttca agatcagcct ggtcaacata gcaagacccc
1561 atctctacaa aaaagaaaaa aattagcaag gcatgtggca tgtgcctgta gtccctgcta
1621 ctcaggaggc tgaggcagga ggatcacttg agcccaggag ttcaaggctg cagtgagcca
1681 taatcctgca ctgtagcctg ggtgacagag tgagtccccc atctcgaaaa aaaaagaact
1741 agtttaaatt ctgactgtat cactgaaagg ctgtgtagct gtgtgaccgt aagcaagtca
1801 cttaactcca gattctcagt gctgtcatct ataaacaggg atgaatgaat atacacctca
1861 gagttgttaa gaatccaatg agaaaatcac gggtaaccct tatataaatg gttgtgaaac
1921 atttcaaaga tacaagcatc cttggccttt gcagcccaga atcatccctc cacatttttc
1981 ctacaatcca accacatcaa gaaatgataa ctgctcagaa agtttatcaa tatttaccaa
2041 aactcatgga tttaaaataa acattaagtt tctacaaaaa aaaaaaaaaa aaaaaaaaaa
2101 aaaaaaaaaa aaaaaaaaaa aaaaaa
//