LOCUS BC043502 2170 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens NIMA (never in mitosis gene a)-related kinase 2, mRNA
(cDNA clone MGC:49922 IMAGE:5541026), complete cds.
ACCESSION BC043502
VERSION BC043502.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2170)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2170)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 89 Row: e Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 49574505.
FEATURES Location/Qualifiers
source 1..2170
/db_xref="H-InvDB:HIT000052826"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:49922 IMAGE:5541026"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2170
/gene="NEK2"
/gene_synonym="HsPK 21"
/gene_synonym="HsPK21"
/gene_synonym="NEK2A"
/gene_synonym="NLK1"
/db_xref="GeneID:4751"
/db_xref="HGNC:HGNC:7745"
/db_xref="MIM:604043"
CDS 140..1477
/gene="NEK2"
/gene_synonym="HsPK 21"
/gene_synonym="HsPK21"
/gene_synonym="NEK2A"
/gene_synonym="NLK1"
/codon_start=1
/product="NIMA (never in mitosis gene a)-related kinase 2"
/protein_id="AAH43502.2"
/db_xref="GeneID:4751"
/db_xref="HGNC:HGNC:7745"
/db_xref="MIM:604043"
/translation="MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSM
TEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGT
KERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDF
GLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTA
FSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVA
DEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQEL
CVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKE
NIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR"
BASE COUNT 683 a 393 c 498 g 596 t
ORIGIN
1 cggggcccaa ggcaggggtg gcgggtcagt gctgctcggg ggcttctcca tccaggtccc
61 tggagttcct ggtccctgga gctccgcact tggcggcgca acctgcgtga ggcagcgcga
121 ctctggcgac tggccggcca tgccttcccg ggctgaggac tatgaagtgt tgtacaccat
181 tggcacaggc tcctacggcc gctgccagaa gatccggagg aagagtgatg gcaagatatt
241 agtttggaaa gaacttgact atggctccat gacagaagct gagaaacaga tgcttgtttc
301 tgaagtgaat ttgcttcgtg aactgaaaca tccaaacatc gttcgttact atgatcggat
361 tattgaccgg accaatacaa cactgtacat tgtaatggaa tattgtgaag gaggggatct
421 ggctagtgta attacaaagg gaaccaagga aaggcaatac ttagatgaag agtttgttct
481 tcgagtgatg actcagttga ctctggccct gaaggaatgc cacagacgaa gtgatggtgg
541 tcataccgta ttgcatcggg atctgaaacc agccaatgtt ttcctggatg gcaagcaaaa
601 cgtcaagctt ggagactttg ggctagctag aatattaaac catgacacga gttttgcaaa
661 aacatttgtt ggcacacctt attacatgtc tcctgaacaa atgaatcgca tgtcctacaa
721 tgagaaatca gatatctggt cattgggctg cttgctgtat gagttatgtg cattaatgcc
781 tccatttaca gcttttagcc agaaagaact cgctgggaaa atcagagaag gcaaattcag
841 gcgaattcca taccgttact ctgatgaatt gaatgaaatt attacgagga tgttaaactt
901 aaaggattac catcgacctt ctgttgaaga aattcttgag aaccctttaa tagcagattt
961 ggttgcagac gagcaaagaa gaaatcttga gagaagaggg cgacaattag gagagccaga
1021 aaaatcgcag gattccagcc ctgtattgag tgagctgaaa ctgaaggaaa ttcagttaca
1081 ggagcgagag cgagctctca aagcaagaga agaaagattg gagcagaaag aacaggagct
1141 ttgtgttcgt gagagactag cagaggacaa actggctaga gcagaaaatc tgttgaagaa
1201 ctacagcttg ctaaaggaac ggaagttcct gtctctggca agtaatccag aacttcttaa
1261 tcttccatcc tcagtaatta agaagaaagt tcatttcagt ggggaaagta aagagaacat
1321 catgaggagt gagaattctg agagtcagct cacatctaag tccaagtgca aggacctgaa
1381 gaaaaggctt cacgctgccc agctgcgggc tcaagccctg tcagatattg agaaaaatta
1441 ccaactgaaa agcagacaga tcctgggcat gcgctagcca ggtagagaga cacagagctg
1501 tgtacaggat gtaatattac caacctttaa agactgatat tcaaatgctg tagtgttgaa
1561 tacttggttc catgagccat gcctttctgt atagtacaca tgatatttcg gaattggttt
1621 tactgttctt cagcaactat tgtacaaaat gttcacattt aatttttctt tcttctttta
1681 agaacatatt ataaaaagaa tactttcttg gttgggcttt taatcctgtg tgtgattact
1741 agtaggaaca tgagatgtga cattctaaat cttgggagaa aaaataatgt taggaaaaaa
1801 atatttatgc aggaagagta gcactcactg aatagtttta aatgactgag tggtatgctt
1861 acaattgtca tgtctagatt taaattttaa gtctgagatt ttaaatgttt ttgagcttag
1921 aaaacccagt tagatgcaat ttggtcatta ataccatgac atcttgctta taaatattcc
1981 attgctctgt agttcaaatc tgttagcttt gtgaaaattc atcactgtga tgtttgtatt
2041 cttttttttt tttctgttta acagaatatg agctgtctgt catttaccta cttctttccc
2101 actaaataaa agaattcttc agtttccctg taaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2161 aaaaaaaaaa
//