LOCUS BC043148 3085 bp mRNA linear HUM 17-NOV-2006
DEFINITION Homo sapiens twinfilin, actin-binding protein, homolog 1
(Drosophila), mRNA (cDNA clone MGC:41876 IMAGE:5297068), complete
cds.
ACCESSION BC043148
VERSION BC043148.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3085)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3085)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 76 Row: n Column: 14.
FEATURES Location/Qualifiers
source 1..3085
/db_xref="H-InvDB:HIT000052776"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:41876 IMAGE:5297068"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3085
/gene="TWF1"
/gene_synonym="A6"
/gene_synonym="MGC23788"
/gene_synonym="MGC41876"
/gene_synonym="PTK9"
/db_xref="GeneID:5756"
/db_xref="HGNC:HGNC:9620"
CDS 32..1186
/gene="TWF1"
/gene_synonym="A6"
/gene_synonym="MGC23788"
/gene_synonym="MGC41876"
/gene_synonym="PTK9"
/codon_start=1
/product="twinfilin, actin-binding protein, homolog 1
(Drosophila)"
/protein_id="AAH43148.2"
/db_xref="GeneID:5756"
/db_xref="HGNC:HGNC:9620"
/translation="MTRRGRRRSSHFLGPPAGAAGCTQRRSRELAAAAMSHQTGIQAS
EDVKEIFARARNGKYRLLKISIENEQLVIGSYSQPSDSWDKDYDSFVLPLLEDKQPCY
ILFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYAATRATLKKEFGGGHIKDEVFGTVK
EDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISR
EAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYK
HSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNG
DELTADFLYEEVHPKQHAHKQSFAKPKGPAGKRGIRRLIRGPAETEATTD"
BASE COUNT 1038 a 521 c 590 g 936 t
ORIGIN
1 agcgggcggg gcggccgcgg gtcgcgggcg gatgacgcgc cggggccggc ggaggagcag
61 ccacttcctg gggccgccgg ccggggccgc tggctgcact cagcgccgga gccgggagct
121 agcggccgcc gccatgtccc accagaccgg catccaagca agtgaagatg ttaaagagat
181 ctttgccaga gccagaaatg gaaagtacag acttctgaaa atatctattg aaaatgagca
241 acttgtgatt ggatcatata gtcagccttc agattcctgg gataaggatt atgattcctt
301 tgttttaccc ctgttggagg acaaacaacc atgctatata ttattcaggt tagattctca
361 gaatgcccag ggatatgaat ggatattcat tgcatggtct ccagatcatt ctcatgttcg
421 tcaaaaaatg ttgtatgcag caacaagagc aactctgaag aaggaatttg gaggtggcca
481 cattaaagat gaagtatttg gaacagtaaa ggaagatgta tcattacatg gatataaaaa
541 atacttgctg tcacaatctt cccctgcccc actgactgca gctgaggaag aactacgaca
601 gattaaaatc aatgaggtac agactgacgt gggtgtggac actaagcatc aaacactaca
661 aggagtagca tttcccattt ctcgagaagc ctttcaggct ttggaaaaat tgaataatag
721 acagctcaac tatgtgcagt tggaaataga tataaaaaat gaaattataa ttttggccaa
781 cacaacaaat acagaactga aagatttgcc aaagaggatt cccaaggatt cagctcgtta
841 ccatttcttt ctgtataaac attcccatga aggagactat ttagagtcca tagtttttat
901 ttattcaatg cctggataca catgcagtat aagagagcgg atgctgtatt ctagctgcaa
961 gagccgtctg ctagaaattg tagaaagaca actacaaatg gatgtaatta gaaagatcga
1021 gatagacaat ggggatgagt tgactgcaga cttcctttat gaagaagtac atcccaagca
1081 gcatgcacac aagcaaagtt ttgcaaaacc aaaaggtcct gcaggaaaaa gaggaattcg
1141 aagactaatt aggggcccag cggaaactga agctactact gattaaagtc atcacattaa
1201 acattgtaat actagttttt taaaagtcca gcttttagta caggagaact gaaatcattc
1261 catgttgata taaagtaggg aaaaaaattg tactttttgg aaaatagcac ttttcacttc
1321 tgtgtgtttt taaaattaat gttatagaag actcatgatt tctatttttg agttaaagct
1381 agaaaagggt tcaacataat gtttaatttt gtcacactgt tttcatagcg ttgattccac
1441 acttcaaata cttcttaaaa ttttatacag ttgggccagt tctagaaagt ctgatgtctc
1501 aaagggtaaa cttactactt tcttgtggga cagaaagacc ttaaaatatt catattactt
1561 aatgaatatg ttaaggacca ggctagagta ttttctaagc tggaaactta gtgtgccttg
1621 gaaaaggccg caagttgctt actccgagta gctgtgctag ctctgtcaga ctgtaggatc
1681 atgtctgcaa cttttagaaa tagtgcttta tattgcagca gtcttttata tttgactttt
1741 ttttaatagc attaaaattg cagatcagct cactctgaaa ctttaagggt accagatatt
1801 ttctatactg caggatttct gatgacattg aaagacttta aacagcctta gtaaattatc
1861 tttctaatgc tctgtgaggc caaacattta tgttcagatt gaaatttaaa ttaatatcat
1921 tcaaaaggaa acaaaaaatg ttgagtttta aaaatcagga ttgacttttt tctccaaaac
1981 catacattta tgggcaaatt gtgttcttta tcacttccga gcaaatactc agatttaaaa
2041 ttactttaaa gtcctggtac ttaacaggct aacgtagata aacaccttaa taatctcagt
2101 taatactgta tttcaaaaca catttaactg ttttctaatg ctttgcatta tcagttacaa
2161 cctagagaga ttttgagcct catatttctt tgatacttga aatagaggga gctagaacac
2221 ttaatgttta atctgttaaa cctgctgcaa gagccataac tttgaggcat tttctaaatg
2281 aactgtgggg atccaggatt tgtaatttct tgatctaaac tttatgctgc ataaatcact
2341 tatcggaaat gcacatttca tagtgtgaag cactcatttc taaaccttat tatctaaggt
2401 aatatatgca cctttcagaa atttgtgttc gagtaagtaa agcatattag aataattgtg
2461 ggttgacaga tttttaaaat agaatttaga gtatttgggg ttttgtttgt ttacaaataa
2521 tcagactata atatttaaac atgcaaaata actgacaata atgttgcact tgtttactaa
2581 agatataagt tgttccatgg gtgtacacgt agacagacac acatacaccc aaattattgc
2641 attaagaatc ctggagcaga ccatagctga agctgttatt ttcagtcagg aagactacct
2701 gtcatgaagg tataaaataa tttagaagtg aatgtttttc tgtaccatct atgtgcaatt
2761 atactctaaa ttccactaca ctacattaaa gtaaatggac attccagaat atagatgtga
2821 ttatagtctt aaactaatta ttattaaacc aatgattgct gaaaatcagt gatgcatttg
2881 ttatagagta taactcatcg tttacagtat gttttagttg gcagtatcat acctagatgg
2941 tgaataacat attcccagta aatttatata gcagtgaaga attacatgcc ttctggtgga
3001 cattttataa gtgcatttta tatcacaata aaaatttttt ctctttaaaa aaaaaaaaaa
3061 aaaaaaaaga aaaaaaaaaa aaaaa
//