LOCUS BC041809 1613 bp mRNA linear HUM 21-JUL-2006
DEFINITION Homo sapiens glutamate-cysteine ligase, modifier subunit, mRNA
(cDNA clone MGC:41886 IMAGE:5311598), complete cds.
ACCESSION BC041809
VERSION BC041809.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1613)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1613)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (23-DEC-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 77 Row: o Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 53759142.
FEATURES Location/Qualifiers
source 1..1613
/db_xref="H-InvDB:HIT000052619"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:41886 IMAGE:5311598"
/tissue_type="Brain, hypothalamus"
/clone_lib="NIH_MGC_96"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..1613
/gene="GCLM"
/db_xref="GeneID:2730"
/db_xref="HGNC:HGNC:4312"
/db_xref="MIM:601176"
CDS 237..1061
/gene="GCLM"
/codon_start=1
/product="glutamate-cysteine ligase, modifier subunit"
/protein_id="AAH41809.1"
/db_xref="GeneID:2730"
/db_xref="HGNC:HGNC:4312"
/db_xref="MIM:601176"
/translation="MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELH
DCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNS
SSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIV
AIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPK
ELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS"
BASE COUNT 451 a 371 c 349 g 442 t
ORIGIN
1 agtagccgga gccggagccg cagccaccgg tgccttcctt tcccgccgcc gcccagccgc
61 cgtccggcct ccctcgggcc cgagcgcaga ccaggctcca gccgcgcggc gccggcagcc
121 tcgcgctccc tctcgggtct ctctcgggcc tcgggcaccg cgtcctgtgg ggcggccgcc
181 tgcctgcccg cccgcccgca gccccttcgc tgcgcggccc ctgggcggcc gctgccatgg
241 gcaccgacag ccgcgcggcc aaggcgctcc tggcgcgggc ccgcaccctg cacctgcaga
301 cggggaacct gctgaactgg ggccgcctgc ggaagaagtg cccgtccacg cacagcgagg
361 agcttcatga ttgtatccaa aaaaccttga atgaatggag ttcccaaatc aacccagatt
421 tggtcaggga gtttccagat gtcttggaat gcactgtatc tcatgcagta gaaaagataa
481 atcctgatga aagagaagaa atgaaagttt ctgcaaaact gttcattgta gaatcaaact
541 cttcatcatc aactagaagt gcagttgaca tggcctgttc agtccttgga gttgcacagc
601 tggattctgt gatcattgct tcacctccta ttgaagatgg agttaatctt tccttggagc
661 atttacagcc ttactgggag gaattagaaa acttagttca gagcaaaaag attgttgcca
721 taggtacctc tgatctagac aaaacacagt tggaacagct gtatcagtgg gcacaggtaa
781 aaccaaatag taaccaagtt aatcttgcct cctgctgtgt gatgccacca gatttgactg
841 catttgctaa acaatttgac atacagctgt tgactcacaa tgatccaaaa gaactgcttt
901 ctgaagcaag tttccaagaa gctcttcagg aaagcattcc tgacattcaa gcgcacgagt
961 gggtgccgct gtggctactg cggtattcgg tcattgtgaa aagtagagga attatcaaat
1021 caaaaggcta cattttacaa gctaaaagaa ggggttctta actgacttag gagcataact
1081 tacctgtaat ttccttcaat atgagagaaa attgagatgt gtaaaaatct agttactgcc
1141 tgtaaatggt gtcattgagg cagatattct ttcgtcatat ttgacagtat gttgtctgtc
1201 aagttttaaa tacttatctt gcctccatat caatccattc tcatgaacct ctgtattgct
1261 ttccttaaac tattgttttc taattgaaat tgtctataaa gaaaatactt gcaatatatt
1321 tttcctttat ttttatgact aatataaatc aagaaaattt gttgttagat atattttggc
1381 ctaggtatca gggtaatgta tatacatatt ttttatttcc aaaaaaaatt cattaattgc
1441 ttcttaactc ttattataac caagcaattt aattacaatt gttaaaactg aaatactgga
1501 agaagatatt tttcctgtca ttgatgagat atatcagagt aactggagta gctgggattt
1561 actagtagtg taaataaaat tcactcttca atacatgaaa aaaaaaaaaa aaa
//