LOCUS BC041710 6060 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens Rap guanine nucleotide exchange factor (GEF) 1, mRNA
(cDNA clone MGC:49019 IMAGE:6051007), complete cds.
ACCESSION BC041710
VERSION BC041710.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 6060)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 6060)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (23-DEC-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 84 Row: o Column: 8.
FEATURES Location/Qualifiers
source 1..6060
/db_xref="H-InvDB:HIT000052600"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:49019 IMAGE:6051007"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..6060
/gene="RAPGEF1"
/gene_synonym="C3G"
/db_xref="GeneID:2889"
/db_xref="HGNC:HGNC:4568"
/db_xref="MIM:600303"
CDS 39..3326
/gene="RAPGEF1"
/gene_synonym="C3G"
/codon_start=1
/product="Rap guanine nucleotide exchange factor (GEF) 1"
/protein_id="AAH41710.1"
/db_xref="GeneID:2889"
/db_xref="HGNC:HGNC:4568"
/db_xref="MIM:600303"
/translation="MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKI
KRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRS
QRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQND
PRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVK
ELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLP
DATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLP
VGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQC
SRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQL
PLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISG
EDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKN
KHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELA
PPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEE
VDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTT
YRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILK
LLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHD
FHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSI
IMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGL
AEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKR
WQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRK
TDREEKT"
BASE COUNT 1336 a 1761 c 1697 g 1266 t
ORIGIN
1 ctggggctgg gaagggccct gtgtcctctg gctttaaaat gggcaatgct attgaaaaac
61 agaagccctt gaaacgaagt catctgtacc cctggaaaca agactctcag cgttctcatc
121 tctcttcctt caccatgaag ctgatggaca aattccactc acccaaaatc aagagaacgc
181 catcaaagaa gggaaaacca gctgaggtgt ccgtaaagat tccagagaag cctgtgaaca
241 aagaggcaac agacagattt ctaccagagg gctaccctct ccccttggat ctggagcagc
301 aggcagtaga atttatgtcc accagtgctg tggcttccag gtctcaaagg cagaagaacc
361 tgagctggct ggaggagaaa gagaaggaag ttgtcagtgc cctgcgctac tttaagacca
421 ttgtggacaa aatggcaatt gataagaagg tactggagat gcttccaggg tcagccagca
481 aggtgctgga ggccatctta cccctggtgc agaacgatcc tcgaattcag cacagctcag
541 ccctctcttc ctgctatagc cgagtgtacc aaagcctcgc caacctcatt cgctggtctg
601 accaagtgat gctggaaggc gtgaactcag aagacaagga gatggtgacg actgtgaagg
661 gggtcatcaa ggctgtgctg gatggagtga aggagctggt caggctcacc atcgagaagc
721 agggacgtcc gtctccgacg agccccgtga agcccagttc ccctgccagc aagcctgatg
781 gcccagcaga gctccccctg acagaccgcg aggtagagat cctaaacaag acgactggga
841 tgtcacagtc aactgagctc ctcccagatg ccacggatga agaggtcgcg ccccccaagc
901 ctcctctgcc tggcattcgg gtggttgata atagtcctcc accagcattg ccacccaaga
961 aaagacagtc ggcgccgtcc cctacccgag tggctgtggt ggcccccatg agccgagcca
1021 ccagtggctc cagtttgcct gttggaatca ataggcagga ttttgatgtt gactgttacg
1081 cacagaggcg actgtcagga ggcagccact catatggtgg agagtcgccc cgcctctccc
1141 cctgcagcag cataggcaag ctcagcaagt cagacgagca gctgtcctct ctggacaggg
1201 acagtgggca gtgctcccgg aacacaagct gtgaaacact agaccactat gatcccgact
1261 atgaattcct ccagcaagac ctctctaacg cagaccagat acctcagcag acggcctgga
1321 accttagccc gttgccagag tctttggggg agtctgggtc tccatttctt ggccctcctt
1381 tccagctgcc tcttggcggc catccccagc cagacggacc tctggcccca gggcagcaga
1441 cagatacgcc acctgctctc cccgagaaga agcgcaggag cgcagcctcc cagacggcgg
1501 acggctctgg ctgcagggtg tcctacgagc ggcatccctc gcagtatgac aacatctctg
1561 gggaggacct gcagagcaca gccccgatcc catccgtccc ctacgcgccc tttgctgcta
1621 ttctgccctt tcagcatgga ggttcctcag cccctgtcga atttgtgggt gattttactg
1681 ctcctgagtc aaccggtgac ccagaaaaac cacctcctct accagagaag aaaaacaaac
1741 acatgctggc ctacatgcag ttgctggagg actactcgga gccgcagccc tctatgttct
1801 accagacgcc acagaacgag cacatctacc agcagaagaa caagctcctc atggaggtat
1861 acggcttcag cgactccttc agtggggtgg actccgtgca ggagctggcc ccgccgcccg
1921 ccctaccccc caagcagcgg cagctggagc caccggctgg gaaagacgga catcccagag
1981 atccctcagc ggtcagcggc gtccctggga aggacagcag agacggcagt gagagggccc
2041 caaagtcacc agatgctctg gagtcggctc agtcggagga ggaagtggac gagctgtccc
2101 tcattgacca caacgaaatt atgtccaggc tgacgctcaa gcaggagggt gatgacgggc
2161 cggacgtccg cggaggatct ggggacatct tactggtcca tgctactgag actgacagga
2221 aagatttggt gttgtactgc gaggcattcc tgaccaccta caggaccttc atctccccag
2281 aggagctcat caagaagctg cagtacagat atgagaaatt ctctcccttt gccgacacat
2341 tcaagaagcg cgtcagcaag aacacgttct tcgtgctggt acgggtggtg gatgagctct
2401 gcctggtgga gttgacagaa gagatcctga agctgctgat ggaactggtc ttccgcctgg
2461 tgtgcaatgg ggagctgagc ctggcccgtg tgctccggaa gaacatcctg gacaaggtgg
2521 accagaagaa gctactcagg tgtgccacct ccagccagcc cctggcagcc cggggggtag
2581 cagccaggcc ggggaccttg cacgactttc acagccatga gatagcggag cagctaacgc
2641 tgctggatgc tgagctcttc tataaaatag agattcctga ggttttgctt tgggcaaaag
2701 agcagaatga ggagaagagc cccaacttga cccagttcac ggagcacttc aacaacatgt
2761 cctactgggt ccggtccata atcatgttac aggaaaaggc ccaggacagg gaacggctgc
2821 tcttgaagtt catcaagatc atgaagcact tgcggaagct gaataacttc aactcctact
2881 tggccatcct ctctgccctg gactcggcgc ccatccgcag gctggagtgg cagaagcaga
2941 cttcagaggg cctggccgag tactgcacac tgatcgacag ctcgtcctcc ttccgagcct
3001 accgggccgc cctctcggag gtggaaccgc cgtgcatccc gtacctgggg ctgatcctgc
3061 aggacctgac cttcgttcac ctgggaaacc cagactacat cgacgggaaa gtgaacttct
3121 ccaagcggtg gcagcagttc aacatcctcg acagcatgcg ctgcttccag caggcgcact
3181 atgacatgcg gaggaacgac gacattataa acttcttcaa tgacttcagt gaccacctgg
3241 ctgaggaggc cctatgggaa ctgtctctga aaattaaacc caggaacata acaaggagaa
3301 aaacagaccg ggaagagaag acctaggagc agacgccggg atccaggaga atgctcgagg
3361 ggcgcagagg gcagctccca gaccggagag gaccttggac ctgttaggcg catggcagga
3421 gtcccggcct cggagccatg aggctggcca gccctcagcg gggccgggcg ggagctggag
3481 cctgccagcc gcttcctgcc tccttcctct gtgggagcag acccgtgggc ctcagggcag
3541 ccagcaggca ggtcttgttg ccaatttaca aaccggtggt tttctggttt ggttttgttt
3601 tctgctttta cttccatctc tcccctcttg accttccacc cactcccctc cagggagaga
3661 gcagcagaga cctcatcagc agaccaagga agtggtgggt gctccccctc cctaagctcc
3721 agggtccctg aatcttctga aatctcaaat gagtggaggc ctcctggggt ggcctgtcct
3781 gcaggggccc tggaatgggg gcaagcagct gggtgggcag aatgcagagt agactcgggg
3841 gaggatcctt tcactttccg cttccccttc tgatgcatgg aggatggtgt gagcttttca
3901 gcaggcccgg aaaggtacgc aggtgacgcc ttagcagccc cgcagctggt gctctgcccc
3961 gcggtactgg cgccatcagg gcctcccttg cccgcctgag agcagcagca gtctctgtca
4021 tcccgtcgcc ccttaccccc caccccaggc cactgggccc ctcccacacc acctggggag
4081 ctgagaagag gaggctggag taagggagga cttgatcatc caagaaatac tttttattgc
4141 tgggagtctt ctgaacctca ccaaactgag gccagagctg agctcctggg ggagttaatt
4201 cagaggggag aggccagcac ctccctcctc cattgctcgc tgtgtgcctt aaactccatc
4261 tcatgtccct ccccatcccc tggctttccc tccctccttg ccccatcctg ggccagccag
4321 cagggctcct cctctggctc ttcagacctt tcagccagtg ctgtcagtgc ccctgggagg
4381 gaagggcatc cctgaggcac ccgaatggtc cctcagggtg cagggaggca gaagcctggc
4441 cacagaggag cctcctaagg cagcagctgc agcaagcgca ccctctcccc actctcccca
4501 cgccagagcg gcttccagag cagatgctgt ttccatcctc ctcgtcaaaa ccattctcgc
4561 tgctgagctt gacaatctgg gcaaggcttg tggggcgctt gacaaacaga atctgccctg
4621 tgccgcctgg ttccgtggcc tccagcatga gcctgcaggc agggcgctgc gggaacccag
4681 ttgtgctgcc ccagcccatg cctccgggtc tgctgtgcat gaatgagtgc tcacttgtcc
4741 cgggtttagg acgtggtcaa gtgaacagca gggtctaact gtgcttactt agcccagttc
4801 aaacagaaca aaggaaaaat atagaaagca acatctgttg atcatttagg ttttttttta
4861 aaccaccatg tcactttgag tccttcatgg gtttttgaac agcatttatc aagaagaaaa
4921 tgtgggcttt ttcccctctc ccgtgttttg tttgtcctgt agatagaggg aggaaagccg
4981 tgcagtggca ggcgggaccc cctctggtgg cgggaccccc tcttgcggtg gtcttgcggg
5041 gccagccggg acctgtcact ttattattta aggagtgtgt gtgtagagtc gctggcttat
5101 taacagtatt gtgtgtgggt tgggttttta gtttgttcct tctttttgaa gtcccttcat
5161 ttcaatcctt gactctctct ccccttccct tgcccagctc tgttgaatgc tgctgtgcgc
5221 gtgtgagggc cgctctgcac acagggccct tgggttgtgt gaactgaaat tctccctgta
5281 tttgtgagac tcgcaggagt ccccatctgt agcacaggca atgccagtgc catgctgcag
5341 cctcagaaac caggcctctc actccagcag caggcagaac cgtgtctgtg gtcgggtgct
5401 gtccacagct ctgtctgcct tgttcttggg cttgagctgg atagaggtgg ggtctcttca
5461 ccttccctga attcagaaca gaccctgtgc ctggccccag tgtgcccagg caattcccca
5521 ggccctcatt gggagccctt ggtgttctga gcagcagggc ccaggcagca catgagcagt
5581 gcccaggggc tccctgcgtg aggacggcaa ggtgcgatgt atgtctaact tattgatggc
5641 aggcagcccc ctgtgccccc taagcctggc cctggttatt gctgagctct gtgctcagtg
5701 ctgcggcctg gccgtggctc gtctgttcct ttggggggcc cgggcgggtt gtgggaatca
5761 gtcttcacag acagacgtga gccaggcgga ggactcgttc cttgcagagg tcagtcctca
5821 cctgcaggtg tcggggtggg ggggggcaag gaggggcagg cacacaccat gtctgacctg
5881 aacccgattc tggggagcat cttcccgctc cggccccacg acctccacag ggttacattg
5941 taatatatat gccccagcta acctgtctga tggtggcatc ttcctgcaga catttcaaac
6001 atgtaacttt tatatgaaaa aaaataaaca cagatgaaag ctgccaaaaa aaaaaaaaaa
//