LOCUS BC040540 4782 bp mRNA linear HUM 04-AUG-2008
DEFINITION Homo sapiens retinoblastoma 1, mRNA (cDNA clone MGC:39656
IMAGE:5267622), complete cds.
ACCESSION BC040540
VERSION BC040540.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4782)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4782)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 63 Row: f Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4506434.
FEATURES Location/Qualifiers
source 1..4782
/db_xref="H-InvDB:HIT000052423"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:39656 IMAGE:5267622"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4782
/gene="RB1"
/gene_synonym="p105-Rb"
/gene_synonym="pp110"
/gene_synonym="pRb"
/gene_synonym="RB"
/db_xref="GeneID:5925"
/db_xref="HGNC:HGNC:9884"
/db_xref="MIM:180200"
CDS 163..2949
/gene="RB1"
/gene_synonym="p105-Rb"
/gene_synonym="pp110"
/gene_synonym="pRb"
/gene_synonym="RB"
/codon_start=1
/product="retinoblastoma 1"
/protein_id="AAH40540.1"
/db_xref="GeneID:5925"
/db_xref="HGNC:HGNC:9884"
/db_xref="MIM:180200"
/translation="MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPL
VRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGIC
IFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFA
LFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQL
MLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEV
LCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDAR
LFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSA
SDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGV
RLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDS
GTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSD
SPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNS
TANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWT
LFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFK
RVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLR
IPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSD
RVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQ
KMNDSMDTSNKEEK"
BASE COUNT 1526 a 892 c 877 g 1487 t
ORIGIN
1 tgttgccggg cgggggaggg cgcgtccggt ttttctcagg ggacgttgaa attatttttg
61 taacgggagt cgggagagga cggggcgtgc cccgacgtgc gcgcgcgtcg tcctccccgg
121 cgctcctcca cagctcgctg gctcccgccg cggaaaggcg tcatgccgcc caaaaccccc
181 cgaaaaacgg ccgccaccgc cgccgctgcc gccgcggaac ccccggcacc gccgccgccg
241 ccccctcctg aggaggaccc agagcaggac agcggcccgg aggacctgcc tctcgtcagg
301 cttgagtttg aagaaacaga agaacctgat tttactgcat tatgtcagaa attaaagata
361 ccagatcatg tcagagagag agcttggtta acttgggaga aagtttcatc tgtggatgga
421 gtattgggag gttatattca aaagaaaaag gaactgtggg gaatctgtat ctttattgca
481 gcagttgacc tagatgagat gtcgttcact tttactgagc tacagaaaaa catagaaatc
541 agtgtccata aattctttaa cttactaaaa gaaattgata ccagtaccaa agttgataat
601 gctatgtcaa gactgttgaa gaagtatgat gtattgtttg cactcttcag caaattggaa
661 aggacatgtg aacttatata tttgacacaa cccagcagtt cgatatctac tgaaataaat
721 tctgcattgg tgctaaaagt ttcttggatc acatttttat tagctaaagg ggaagtatta
781 caaatggaag atgatctggt gatttcattt cagttaatgc tatgtgtcct tgactatttt
841 attaaactct cacctcccat gttgctcaaa gaaccatata aaacagctgt tatacccatt
901 aatggttcac ctcgaacacc caggcgaggt cagaacagga gtgcacggat agcaaaacaa
961 ctagaaaatg atacaagaat tattgaagtt ctctgtaaag aacatgaatg taatatagat
1021 gaggtgaaaa atgtttattt caaaaatttt atacctttta tgaattctct tggacttgta
1081 acatctaatg gacttccaga ggttgaaaat ctttctaaac gatacgaaga aatttatctt
1141 aaaaataaag atctagatgc aagattattt ttggatcatg ataaaactct tcagactgat
1201 tctatagaca gttttgaaac acagagaaca ccacgaaaaa gtaaccttga tgaagaggtg
1261 aatgtaattc ctccacacac tccagttagg actgttatga acactatcca acaattaatg
1321 atgattttaa attcagcaag tgatcaacct tcagaaaatc tgatttccta ttttaacaac
1381 tgcacagtga atccaaaaga aagtatactg aaaagagtga aggatatagg atacatcttt
1441 aaagagaaat ttgctaaagc tgtgggacag ggttgtgtcg aaattggatc acagcgatac
1501 aaacttggag ttcgcttgta ttaccgagta atggaatcca tgcttaaatc agaagaagaa
1561 cgattatcca ttcaaaattt tagcaaactt ctgaatgaca acatttttca tatgtcttta
1621 ttggcgtgcg ctcttgaggt tgtaatggcc acatatagca gaagtacatc tcagaatctt
1681 gattctggaa cagatttgtc tttcccatgg attctgaatg tgcttaattt aaaagccttt
1741 gatttttaca aagtgatcga aagttttatc aaagcagaag gcaacttgac aagagaaatg
1801 ataaaacatt tagaacgatg tgaacatcga atcatggaat cccttgcatg gctctcagat
1861 tcacctttat ttgatcttat taaacaatca aaggaccgag aaggaccaac tgatcacctt
1921 gaatctgctt gtcctcttaa tcttcctctc cagaataatc acactgcagc agatatgtat
1981 ctttctcctg taagatctcc aaagaaaaaa ggttcaacta cgcgtgtaaa ttctactgca
2041 aatgcagaga cacaagcaac ctcagccttc cagacccaga agccattgaa atctacctct
2101 ctttcactgt tttataaaaa agtgtatcgg ctagcctatc tccggctaaa tacactttgt
2161 gaacgccttc tgtctgagca cccagaatta gaacatatca tctggaccct tttccagcac
2221 accctgcaga atgagtatga actcatgaga gacaggcatt tggaccaaat tatgatgtgt
2281 tccatgtatg gcatatgcaa agtgaagaat atagacctta aattcaaaat cattgtaaca
2341 gcatacaagg atcttcctca tgctgttcag gagacattca aacgtgtttt gatcaaagaa
2401 gaggagtatg attctattat agtattctat aactcggtct tcatgcagag actgaaaaca
2461 aatattttgc agtatgcttc caccaggccc cctaccttgt caccaatacc tcacattcct
2521 cgaagccctt acaagtttcc tagttcaccc ttacggattc ctggagggaa catctatatt
2581 tcacccctga agagtccata taaaatttca gaaggtctgc caacaccaac aaaaatgact
2641 ccaagatcaa gaatcttagt atcaattggt gaatcattcg ggacttctga gaagttccag
2701 aaaataaatc agatggtatg taacagcgac cgtgtgctca aaagaagtgc tgaaggaagc
2761 aaccctccta aaccactgaa aaaactacgc tttgatattg aaggatcaga tgaagcagat
2821 ggaagtaaac atctcccagg agagtccaaa tttcagcaga aactggcaga aatgacttct
2881 actcgaacac gaatgcaaaa gcagaaaatg aatgatagca tggatacctc aaacaaggaa
2941 gagaaatgag gatctcagga ccttggtgga cactgtgtac acctctggat tcattgtctc
3001 tcacagatgt gactgtataa ctttcccagg ttctgtttat ggccacattt aatatcttca
3061 gctctttttg tggatataaa atgtgcagat gcaattgttt gggtgattcc taagccactt
3121 gaaatgttag tcattgttat ttatacaaga ttgaaaatct tgtgtaaatc ctgccattta
3181 aaaagttgta gcagattgtt tcctcttcca aagtaaaatt gctgtgcttt atggatagta
3241 agaatggccc tagagtggga gtcctgataa cccaggcctg tctgactact ttgccttctt
3301 ttgtagcata taggtgatgt ttgctcttgt ttttattaat ttatatgtat atttttttaa
3361 tttaacatga acacccttag aaaatgtgtc ctatctatct tccaaatgca atttgattga
3421 ctgcccattc accaaaatta tcctgaactc ttctgcaaaa atggatatta ttagaaatta
3481 gaaaaaaatt actaatttta cacattagat tttattttac tattggaatc tgatatactg
3541 tgtgcttgtt ttataaaatt ttgcttttaa ttaaataaaa gctggaagca aagtataacc
3601 atatgatact atcatactac tgaaacagat ttcatacctc agaatgtaaa agaacttact
3661 gattattttc ttcatccaac ttatgttttt aaatgaggat tattgatagt actcttggtt
3721 tttataccat tcagatcact gaatttataa agtacccatc tagtacttga aaaagtaaag
3781 tgttctgcca gatcttaggt atagaggacc ctaacacagt atatcccaag tgcactttct
3841 aatgtttctg ggtcctgaag aattaagata caaattaatt ttactccata aacagactgt
3901 taattatagg agccttaatt tttttttcat agagatttgt ctaattgcat ctcaaaatta
3961 ttctgccctc cttaatttgg gaaggtttgt gttttctctg gaatggtaca tgtcttccat
4021 gtatcttttg aactggcaat tgtctattta tcttttattt ttttaagtca gtatggtcta
4081 acactggcat gttcaaagcc acattatttc tagtccaaaa ttacaagtaa tcaagggtca
4141 ttatgggtta ggcattaatg tttctatctg attttgtgca aaagcttcaa attaaaacag
4201 ctgcattaga aaaagaggcg cttctcccct cccctacacc taaaggtgta tttaaactat
4261 cttgtgtgat taacttattt agagatgctg taacttaaaa taggggatat ttaaggtagc
4321 ttcagctagc ttttaggaaa atcactttgt ctaactcaga attattttta aaaagaaatc
4381 tggtcttgtt agaaaacaaa attttatttt gtgctcattt aagtttcaaa cttactattt
4441 tgacagttat tttgataaca atgacactag aaaacttgac tccatttcat cattgtttct
4501 gcatgaatat catacaaatc agttagtttt taggtcaagg gcttactatt tctgggtctt
4561 ttgctactaa gttcacatta gaattagtgc cagaatttta ggaacttcag agatcgtgta
4621 ttgagatttc ttaaataatg cttcagatat tattgcttta ttgctttttt gtattggtta
4681 aaactgtaca tttaaaattg ctatgttact attttctaca attaatagtt tgtctatttt
4741 aaaataaatt agttgttaag agtctcaaaa aaaaaaaaaa aa
//