LOCUS BC040275 3950 bp mRNA linear HUM 06-OCT-2003
DEFINITION Homo sapiens Ras protein-specific guanine nucleotide-releasing
factor 1, mRNA (cDNA clone MGC:35460 IMAGE:5192914), complete cds.
ACCESSION BC040275
VERSION BC040275.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3950)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3950)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (27-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 50 Row: l Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 24797098.
FEATURES Location/Qualifiers
source 1..3950
/db_xref="H-InvDB:HIT000052360"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:35460 IMAGE:5192914"
/tissue_type="Brain, adult, 6 pooled whole brains"
/clone_lib="NIH_MGC_114"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3950
/gene="RASGRF1"
/gene_synonym="CDC25"
/gene_synonym="CDC25L"
/gene_synonym="GNRP"
/gene_synonym="GRF1"
/gene_synonym="GRF55"
/gene_synonym="H-GRF55"
/db_xref="GeneID:5923"
/db_xref="MIM:606600"
CDS 267..3812
/gene="RASGRF1"
/gene_synonym="CDC25"
/gene_synonym="CDC25L"
/gene_synonym="GNRP"
/gene_synonym="GRF1"
/gene_synonym="GRF55"
/gene_synonym="H-GRF55"
/codon_start=1
/product="RASGRF1 protein"
/protein_id="AAH40275.1"
/db_xref="GeneID:5923"
/db_xref="MIM:606600"
/translation="MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWF
ALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHE
NQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQL
RQQIEDGEIEIERLKAEITSLLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLC
RRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASS
KKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQ
EFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTL
HELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEIL
LDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGG
KLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVA
SSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFS
KTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIY
KKPISAIPARSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRK
LSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPD
EGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTETSPTKSPTTPKSVKNKN
SSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLAS
AGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMH
DPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQL
TLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDIN
ARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQVRAGGWHH
CLPGAGLGLGQEDPWAGHWASTSGQRTGLRSNTPR"
misc_feature 336..656
/gene="RASGRF1"
/gene_synonym="CDC25"
/gene_synonym="CDC25L"
/gene_synonym="GNRP"
/gene_synonym="GRF1"
/gene_synonym="GRF55"
/gene_synonym="H-GRF55"
/note="PH; Region: Pleckstrin homology domain. Domain
commonly found in eukaryotic signalling proteins. The
domain family possesses multiple functions including the
abilities to bind inositol phosphates, and various
proteins. PH domains have been found to possess inserted
domains (such as in PLC gamma, syntrophins) and to be
inserted within other domains. Mutations in Brutons
tyrosine kinase (Btk) within its PH domain cause X-linked
agammaglobulinaemia (XLA) in patients. Point mutations
cluster into the positively charged end of the molecule
around the predicted binding site for phosphatidylinositol
lipids"
/db_xref="CDD:smart00233"
misc_feature 996..1541
/gene="RASGRF1"
/gene_synonym="CDC25"
/gene_synonym="CDC25L"
/gene_synonym="GNRP"
/gene_synonym="GRF1"
/gene_synonym="GRF55"
/gene_synonym="H-GRF55"
/note="RhoGEF; Region: RhoGEF domain. Guanine nucleotide
exchange factor for Rho/Rac/Cdc42-like GTPases Also called
Dbl-homologous (DH) domain. It appears that pfam00169
domains invariably occur C-terminal to RhoGEF/DH domains"
/db_xref="CDD:pfam00621"
misc_feature 2151..2312
/gene="RASGRF1"
/gene_synonym="CDC25"
/gene_synonym="CDC25L"
/gene_synonym="GNRP"
/gene_synonym="GRF1"
/gene_synonym="GRF55"
/gene_synonym="H-GRF55"
/note="RasGEFN; Region: Guanine nucleotide exchange factor
for Ras-like GTPases"
/db_xref="CDD:pfam00618"
misc_feature 3015..3242
/gene="RASGRF1"
/gene_synonym="CDC25"
/gene_synonym="CDC25L"
/gene_synonym="GNRP"
/gene_synonym="GRF1"
/gene_synonym="GRF55"
/gene_synonym="H-GRF55"
/note="RasGEFN; Region: Guanine nucleotide exchange factor
for Ras-like GTPases"
/db_xref="CDD:smart00229"
misc_feature 3324..3680
/gene="RASGRF1"
/gene_synonym="CDC25"
/gene_synonym="CDC25L"
/gene_synonym="GNRP"
/gene_synonym="GRF1"
/gene_synonym="GRF55"
/gene_synonym="H-GRF55"
/note="RasGEF; Region: RasGEF domain. Guanine nucleotide
exchange factor for Ras-like small GTPases"
/db_xref="CDD:pfam00617"
BASE COUNT 1029 a 1131 c 1077 g 713 t
ORIGIN
1 cggcaccgga ggagggtcgg gcggcatctt ccgggtactg gggctctgcg gagcggagaa
61 gaggttccag cggggaatgg tatatctgga ttcaagaagc cgcggctcgg cgccagatcc
121 tggagtgtag atatttgggg ggaggggaga gctagaggga gcgagcgagc gagcgccaga
181 gagaggcagc gcgcagcgcg cacggacggg gggctgctgc gcggagaaga tgtaagcgcg
241 tggggtctcg ctggcctctg agcaccatgc agaaggggat ccggctgaat gatggccacg
301 tcgcgtccct gggactgctg gcgcgcaagg acggcacgcg caaaggctac ctgagcaagc
361 ggagttcgga caacacaaaa tggcaaacca agtggttcgc gctgctgcag aacctgctct
421 tctacttcga gagcgactcg agctcgcggc cctcggggct ttacctgctg gagggctgcg
481 tctgcgaccg cgcgccctcc cccaagccgg cgctgtcggc caaggagccg ctggagaaac
541 agcattactt cacggtgaac ttcagccatg agaaccagaa agccttggag ctgaggacag
601 aggacgcaaa agattgtgac gaatgggtgg cagccattgc acatgccagc tacaggaccc
661 tcgccacaga gcatgaggca ttaatgcaga aatacctgca cctgctgcag atcgtggaga
721 cagagaagac cgtggccaag cagcttcggc agcagatcga ggatggggag atcgagatcg
781 agcggctgaa ggcagagatc acatccctgc tcaaggacaa tgagcgcatc cagtccaccc
841 agactgtcgc ccccaacgat gaagacagcg acatcaagaa aattaagaag gtgcagagct
901 tcctgcgggg ctggctgtgc cggcggaagt ggaagaccat catccaggac tacatccggt
961 caccccatgc tgacagcatg cgcaagagga accaggtggt gttcagcatg ctggaggctg
1021 aggctgagta cgtgcagcag ctgcacatcc ttgtcaacaa tttcctgcgc ccgctgcgga
1081 tggccgccag ctccaagaag cctcccatca cacacgacga cgtcagcagc atcttcctga
1141 acagcgaaac catcatgttt ttacatcaga tcttttacca aggcctgaag gcccgcatct
1201 ccagctggcc cacgctggtc ctggctgacc tatttgacat cctgctgccc atgctcaaca
1261 tctaccaaga gttcgtccgc aaccaccagt acagcctgca gatcctggcc cactgcaagc
1321 agaaccgtga cttcgacaag ctgctgaagc actacgaggc caagcctgac tgcgaggaga
1381 ggacgctgga gaccttcctc acctacccca tgttccagat ccccaggtac atcctgaccc
1441 tccatgagct cctggcccac acgcctcatg agcacgttga gcgcaacagc ctggactacg
1501 ccaagtccaa actggaggag ctgtccagaa taatgcacga tgaagtaagt gagacggaga
1561 acatccggaa aaacctggcc atcgagcgca tgatcatcga aggctgtgag atcctcctgg
1621 acaccagcca gacctttgtg agacaaggtt ccctcattca ggtgcccatg tctgaaaagg
1681 gcaagatcac cagggggcgc ctggggtctc tctccctaaa gaaagagggc gagcgacagt
1741 gcttcctgtt ttctaagcat ctgattatct gtaccagagg ctctggaggg aagcttcact
1801 tgaccaagaa tggagtcata tccctcattg actgcacttt attggaggag ccagaaagca
1861 cggaggagga agccaaagga tccggccaag acatagatca cttggatttt aaaatcgggg
1921 tggagccaaa ggattccccg ccctttacag tcatcctagt ggcctcgtcc agacaggaga
1981 aggcagcgtg gaccagtgac atcagccagt gtgtggataa catccgatgc aatgggctca
2041 tgatgaacgc atttgaagaa aattccaagg tcactgtgcc gcagatgatc aagtccgacg
2101 cctccttata ttgtgatgat gttgacattc gcttcagcaa aaccatgaac tcctgcaaag
2161 tgctgcagat ccgctacgcc agtgtggagc ggctgctgga gaggctgacg gacctgcgct
2221 tcctgagcat cgacttcctc aacaccttcc tgcactccta ccgcgtcttc accaccgcca
2281 tcgtggtcct ggacaagctc attaccatct acaagaagcc tatcagtgcc attcctgcca
2341 ggtcgctgga gctcctgttt gccagtggcc agaacaataa gctcctgtac ggtgaacccc
2401 ccaagtcccc gcgcgccacc cgcaagttct cctcgccgcc acctctgtcc atcaccaaga
2461 catcgtcacc gagccgccgg cggaagctct ccctgaacat ccccatcatc actggcggca
2521 aggccctgga cctggccgcc ctcagctgca actccaatgg ctacaccagc atgtactcgg
2581 ccatgtcacc cttcagcaag gccacgctgg acaccagcaa gctctatgtg tccagcagct
2641 tcaccaacaa gattccagat gagggcgata cgacccctga gaagcccgaa gacccttcag
2701 cgctcagcaa gcagagctca gaagtctcca tgagagagga gtcagatatt gatcaaaacc
2761 agagtgatga tggtgatact gaaacatcac caactaaatc tccaacaaca cccaaatcag
2821 tcaaaaacaa aaattcttca gagttcccac tcttttccta taacaatgga gtcgtcatga
2881 cctcctgtcg tgaactggac aataaccgca gtgccttgtc ggccgcctct gcctttgcca
2941 tagcaaccgc cggggccaac gagggcaccc caaacaagga gaagtaccgg aggatgtcct
3001 tagccagtgc agggtttccc ccagaccaga ggaatggaga caaggagttt gtgatccgca
3061 gagcagccac caatcgtgtc ttgaacgtgc tccgccactg ggtgtccaag cactctcagg
3121 actttgagac caacgatgag ctcaaatgca aggtgatcgg cttcctggaa gaagtcatgc
3181 acgacccgga gctcctgacc caggagcgga aggctgcagc caacatcatc aggactctga
3241 cccaggagga cccaggtgac aaccagatca cgctggagga gatcacgcag atggctgaag
3301 gcgtgaaggc tgagcccttt gaaaaccact cagccctgga gatcgcggag cagctgaccc
3361 tgctagatca cctcgtcttc aagaagattc cttatgagga gttcttcgga caaggatgga
3421 tgaaactgga aaagaatgaa aggacccctt atatcatgaa aaccactaag cacttcaatg
3481 acatcagtaa cttgattgct tcagaaatca tccgcaacga ggacatcaac gccagggtga
3541 gcgccatcga gaagtgggtg gccgtagctg acatatgccg ctgcctccac aactacaatg
3601 ccgtactgga gatcacctcg tccatgaacc gcagtgcaat cttccggctc aaaaagacgt
3661 ggctcaaagt ctctaagcag gtgcgggcag gaggctggca ccactgcctc cctggggctg
3721 gattggggct ggggcaggaa gacccgtggg ctggccactg ggcatccaca agtgggcaaa
3781 ggactgggct gaggtctaac actcccaggt aaaacagaat agcttcccct atatcacctg
3841 ggaccagata gcaggcccac agatatggca gtgagaccgt ggactgtaat ccccacttga
3901 gtaattaata cctcaaagtg gaaagaaaaa aaaaaaaaaa aaaaaaaaaa
//