LOCUS BC039591 1872 bp mRNA linear HUM 26-APR-2004
DEFINITION Homo sapiens Rho GTPase activating protein 25, mRNA (cDNA clone
MGC:48798 IMAGE:5757311), complete cds.
ACCESSION BC039591
VERSION BC039591.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1872)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1872)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (01-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 84 Row: l Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7661881.
FEATURES Location/Qualifiers
source 1..1872
/db_xref="H-InvDB:HIT000052219"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:48798 IMAGE:5757311"
/tissue_type="Blood, adult leukocytes"
/clone_lib="NIH_MGC_118"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1872
/gene="ARHGAP25"
/gene_synonym="KIAA0053"
/db_xref="GeneID:9938"
CDS 242..1618
/gene="ARHGAP25"
/gene_synonym="KIAA0053"
/codon_start=1
/product="ARHGAP25 protein"
/protein_id="AAH39591.1"
/db_xref="GeneID:9938"
/translation="MSLGQSACLFLSIARSRSVMTGEQMAAFHPSSTPNPLERPIKMG
WLKKQRSIVKNWQQRYFVLRAQQLYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGK
FVFEIIPASWDQNRMGQDSYVLMASSQAEMEEWVKFLRRVAGTPCGAVFGQRLDETVA
YEQKFGPHLVPILVEKCAEFILEHGRNEEGIFRLPGQDNLVKQLRDAFDAGERPSFDR
DTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLTNADEAKAQQELMKQLSILPR
DNYSLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQR
VMTMMIRDHEVLFPKSKDIPLSPPAQKNDPKKAPVARSSVGWDATEDLRISRTDSFSS
MVRCREPSCFHWVLPLVQAIPCKACSRVAIWGVLGDAVAVGAAATDSSEHTLKAWPLS
KSSFYWHL"
misc_feature 359..670
/gene="ARHGAP25"
/gene_synonym="KIAA0053"
/note="PH; Region: Pleckstrin homology domain. Domain
commonly found in eukaryotic signalling proteins. The
domain family possesses multiple functions including the
abilities to bind inositol phosphates, and various
proteins. PH domains have been found to possess inserted
domains (such as in PLC gamma, syntrophins) and to be
inserted within other domains. Mutations in Brutons
tyrosine kinase (Btk) within its PH domain cause X-linked
agammaglobulinaemia (XLA) in patients. Point mutations
cluster into the positively charged end of the molecule
around the predicted binding site for phosphatidylinositol
lipids"
/db_xref="CDD:smart00233"
misc_feature 692..1261
/gene="ARHGAP25"
/gene_synonym="KIAA0053"
/note="RhoGAP; Region: GTPase-activator protein for
Rho-like GTPases"
/db_xref="CDD:cd00159"
BASE COUNT 447 a 495 c 507 g 423 t
ORIGIN
1 gaatgacggg gcccgccgcg gtgccggact gcctgtgcac ggggccaccg actgcagcct
61 gggttttatt cttggcctgg ccctgaccgg gagctggccc ctcggctgct tctctggctc
121 gggggggact ttctctggct cagatccgga cccctgaact ggacctggtt gtcgtccccc
181 cgcttctcag ccccctctgg ggttcctctc tcctctccgc ccactctttg ctcactgccc
241 catgtccctc ggtcagtcgg cctgtctgtt cctctctata gctcggtcaa ggagtgtgat
301 gactggcgag cagatggctg ccttccatcc atcgtccacc cccaacccgc tggagaggcc
361 catcaagatg ggctggctga agaagcagag gtccatcgtg aagaactggc agcagaggta
421 ctttgtgctg agggcgcagc agctctacta ctacaaggat gaagaggaca cgaagcccca
481 gggctgcatg tatctaccag gatgtacaat caaggagatc gccacaaacc cagaagaagc
541 tgggaagttt gtctttgaaa tcattccagc ctcatgggac cagaatcgca tgggacagga
601 ctcctatgtc ctcatggcca gctctcaggc ggagatggag gagtgggtta aattcctcag
661 gagagttgct ggcacaccct gtggagcagt gtttggccag cgcttggatg agactgtggc
721 ctatgaacag aaattcggcc cccatctggt gcccatcctg gtggagaaat gtgcagagtt
781 catcctggag cacggccgga atgaagaggg catcttccgt ctgcctgggc aggacaacct
841 ggtgaagcag ctgagagacg cttttgatgc tggggagcgg ccctcctttg acagagacac
901 agatgtgcac actgtggctt ccctgttaaa gctctacctc cgagacctcc cagagcccgt
961 ggttccctgg agccagtacg aagggttcct gctctgtggg cagctcacga atgcggatga
1021 ggcaaaggct cagcaggagt tgatgaagca gctctccatc cttcctcgtg acaactatag
1081 tctcctgagc tacatctgca ggttcctaca tgaaatacag ctgaactgtg ctgttaacaa
1141 gatgagtgtg gacaacctgg ctactgtgat tggtgtgaat ctcatcaggt cgaaggtcga
1201 agaccctgcc gtgatcatga gagggactcc tcagatccaa agagtgatga ctatgatgat
1261 cagagaccat gaagtcctct tccccaagtc caaggatata cccctgtcac cccctgccca
1321 gaaaaatgac cccaagaaag ctccagtggc ccgaagctct gtaggctggg atgccactga
1381 agacctccga atttctagga cagacagctt cagtagcatg gtaaggtgca gagaaccttc
1441 ctgcttccat tgggttcttc ctttagtcca ggccatccca tgtaaggcct gctctcgggt
1501 ggcaatttgg ggagttttag gtgatgcagt ggcagtgggc gctgctgcta cagactcatc
1561 tgaacacacc ttgaaagcct ggcctctttc caaatctagt ttctattggc atctttgagg
1621 ggggtgagtg acaagtgtgc caatcaccca gtgactgagc aatgaggtcc tcactgaagc
1681 ccactctgaa agagagactc aaatacagtc cagagaagtt cagtgggaaa aatgtataca
1741 acattgtaaa tgttttggtc agaattgttg aaaattctgg gtgaacgtcc agagaaaact
1801 ggtttcctgc taacccatga aacgtgattc ctttctcaga gtaaaaaaaa aaaaaaaaaa
1861 aaaaaaaaaa aa
//