LOCUS BC039158 2672 bp mRNA linear HUM 09-DEC-2005
DEFINITION Homo sapiens pyruvate dehydrogenase kinase, isozyme 1, mRNA (cDNA
clone MGC:24867 IMAGE:4778360), complete cds.
ACCESSION BC039158
VERSION BC039158.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2672)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2672)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: James Cleaver, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 29 Row: l Column: 8.
FEATURES Location/Qualifiers
source 1..2672
/db_xref="H-InvDB:HIT000052146"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:24867 IMAGE:4778360"
/tissue_type="Skin, normal"
/clone_lib="NCI_CGAP_Skn3"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6.1"
gene 1..2672
/gene="PDK1"
/db_xref="GeneID:5163"
/db_xref="HGNC:HGNC:8809"
/db_xref="IMGT/GENE-DB:8809"
/db_xref="MIM:602524"
CDS 44..1354
/gene="PDK1"
/codon_start=1
/product="PDK1 protein"
/protein_id="AAH39158.1"
/db_xref="GeneID:5163"
/db_xref="HGNC:HGNC:8809"
/db_xref="IMGT/GENE-DB:8809"
/db_xref="MIM:602524"
/translation="MRLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPG
QVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLL
RTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGV
IEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGS
INPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHM
VFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNY
MYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKAL
STDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA"
BASE COUNT 826 a 510 c 568 g 768 t
ORIGIN
1 gaaacctggc gtactggctg tggcttctct agcgggactc ggcatgaggc tggcgcggct
61 gcttcgcgga gccgccttgg ccggcccggg cccggggctg cgcgccgccg gcttcagccg
121 cagcttcagc tcggactcgg gctccagccc ggcgtccgag cgcggcgttc cgggccaggt
181 ggacttctac gcgcgcttct cgccgtcccc gctctccatg aagcagttcc tggacttcgg
241 atcagtgaat gcttgtgaaa agacctcatt tatgtttctg cggcaagagt tgcctgtcag
301 actggcaaat ataatgaaag aaataagtct ccttccagat aatcttctca ggacaccatc
361 cgttcaattg gtacaaagct ggtatatcca gagtcttcag gagcttcttg attttaagga
421 caaaagtgct gaggatgcta aagctattta tgactttaca gatactgtga tacggatcag
481 aaaccgacac aatgatgtca ttcccacaat ggcccagggt gtgattgaat acaaggagag
541 ctttggggtg gatcctgtca ccagccagaa tgttcagtac tttttggatc gattctacat
601 gagtcgcatt tcaattagaa tgttactcaa tcagcactct ttattgtttg gtggaaaagg
661 caaaggaagt ccatctcatc gaaaacacat tggaagcata aatccaaact gcaatgtact
721 tgaagttatt aaagatggct atgaaaatgc taggcgtctg tgtgatttgt attatattaa
781 ctctcccgaa ctagaacttg aagaactaaa tgcaaaatca ccaggacagc caatacaagt
841 ggtttatgta ccatcccatc tctatcacat ggtgtttgaa cttttcaaga atgcaatgag
901 agccactatg gaacaccatg ccaacagagg tgtttacccc cctattcaag ttcatgtcac
961 gctgggtaat gaggatttga ctgtgaagat gagtgaccga ggaggtggcg ttcctttgag
1021 gaaaattgac agacttttca actacatgta ttcaactgca ccaagacctc gtgttgagac
1081 ctcccgcgca gtgcctctgg ctggttttgg ttatggattg cccatatcac gtctttacgc
1141 acaatacttc caaggagacc tgaagctgta ttccctagag ggttacggga cagatgcagt
1201 tatctacatt aaggctctgt caacagactc aatagaaaga ctcccagtgt ataacaaagc
1261 tgcctggaag cattacaaca ccaaccacga ggctgatgac tggtgcgtcc ccagcagaga
1321 acccaaagac atgacgacgt tccgcagtgc ctagacacac ttgggacatc ggaaaatcca
1381 aatgtggctt ttgtattaaa tttggaagtg tggcccagag ttgctcagaa ttggagcaga
1441 gcctgagacg tatctgcaga tcctgtcatc agctggcaag tccaggagac tgtgtcattt
1501 agagactgtg ttgttagtta tccctcaaca tcttctaagg tggcaggaaa taatattgga
1561 aataacattt taaagtaaaa attttaaagt ttaaagaaga gttttgccac ttaaacaggg
1621 gagctttgtc tggaaaatac actgagttga aacacttcat ccttggaagg attatataag
1681 atgaacagtt gtgataaatg tgtagattag agggatgtga atgggcagtt agtccagtgc
1741 cctcatttaa gaggccaaga tcctgattca gaggaggcat cctttgccca gagctgctta
1801 gctaatctga ccaaatgttg ggaaaaatgt ctcacctaac ccactattcc ttaattatgg
1861 attttgtgaa aaacaataga acatgttaat gagtaattta tattagttcg atgtattaca
1921 attttttagc tttaaattac agttttctta taatgttgaa atgttttaga atcctttgaa
1981 tctaagtatt tgtttcctaa atgaaacatt tgtacaacat ttgatgtttt tacttatgaa
2041 atattctcct cccccaagaa aatttaaact ttttctctct atttaaaagc taagaaatgt
2101 tttaaaggaa aaatgaaatt atcttccttt agcttatttt taaggtaaaa cagcttttta
2161 ctctgttatt gtggtaatgg acagaatatt acatacaaaa atattctggg agagcttttt
2221 cctagttggt tttaaatcat tgtgccacct gaaaggtttt tagattttat aggagctaat
2281 ttgtccacca gcattaatgt aacacagtgt agttatgaaa atatattgaa ggacaggaag
2341 tggacacgaa gtgatttttg taacctgagc agttaatgaa tgtgccaaca ttttctagga
2401 agggacagca agaatattct gctctgtagt taaaatactg gctggctttt gatgtcttca
2461 tgcttaattg tgatcacttt cttgcactgt gatgttttta cgtgaatatg ttgaagtaga
2521 agtctaccat attattttat aaaatgtttt ctgtatggca ataaactgaa aacatggatc
2581 aacccttctt ttgaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2641 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
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