LOCUS BC039060 4781 bp mRNA linear HUM 04-AUG-2008
DEFINITION Homo sapiens retinoblastoma 1, mRNA (cDNA clone MGC:29887
IMAGE:5142020), complete cds.
ACCESSION BC039060
VERSION BC039060.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4781)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4781)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 42 Row: p Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4506434.
FEATURES Location/Qualifiers
source 1..4781
/db_xref="H-InvDB:HIT000052125"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:29887 IMAGE:5142020"
/tissue_type="Cervix, carcinoma"
/clone_lib="NIH_MGC_12"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4781
/gene="RB1"
/gene_synonym="p105-Rb"
/gene_synonym="pp110"
/gene_synonym="pRb"
/gene_synonym="RB"
/db_xref="GeneID:5925"
/db_xref="HGNC:HGNC:9884"
/db_xref="MIM:180200"
CDS 153..2939
/gene="RB1"
/gene_synonym="p105-Rb"
/gene_synonym="pp110"
/gene_synonym="pRb"
/gene_synonym="RB"
/codon_start=1
/product="retinoblastoma 1"
/protein_id="AAH39060.1"
/db_xref="GeneID:5925"
/db_xref="HGNC:HGNC:9884"
/db_xref="MIM:180200"
/translation="MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPL
VRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGIC
IFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFA
LFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQL
MLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEV
LCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDAR
LFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSA
SDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGV
RLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDS
GTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSD
SPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNS
TANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWT
LFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFK
RVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLR
IPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSD
RVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQ
KMNDSMDTSNKEEK"
BASE COUNT 1536 a 889 c 872 g 1484 t
ORIGIN
1 cgggggaggg cgcgtccggt ttttctcagg ggacgttgaa attatttttg taacgggagt
61 cgggagagga cggggcgtgc cccgacgtgc gcgcgcgtcg tcctccccgg cgctcctcca
121 cagctcgctg gctcccgccg cggaaaggcg tcatgccgcc caaaaccccc cgaaaaacgg
181 ccgccaccgc cgccgctgcc gccgcggaac ccccggcacc gccgccgccg ccccctcctg
241 aggaggaccc agagcaggac agcggcccgg aggacctgcc tctcgtcagg cttgagtttg
301 aagaaacaga agaacctgat tttactgcat tatgtcagaa attaaagata ccagatcatg
361 tcagagagag agcttggtta acttgggaga aagtttcatc tgtggatgga gtattgggag
421 gttatattca aaagaaaaag gaactgtggg gaatctgtat ctttattgca gcagttgacc
481 tagatgagat gtcgttcact tttactgagc tacagaaaaa catagaaatc agtgtccata
541 aattctttaa cttactaaaa gaaattgata ccagtaccaa agttgataat gctatgtcaa
601 gactgttgaa gaagtatgat gtattgtttg cactcttcag caaattggaa aggacatgtg
661 aacttatata tttgacacaa cccagcagtt cgatatctac tgaaataaat tctgcattgg
721 tgctaaaagt ttcttggatc acatttttat tagctaaagg ggaagtatta caaatggaag
781 atgatctggt gatttcattt cagttaatgc tatgtgtcct tgactatttt attaaactct
841 cacctcccat gttgctcaaa gaaccatata aaacagctgt tatacccatt aatggttcac
901 ctcgaacacc caggcgaggt cagaacagga gtgcacggat agcaaaacaa ctagaaaatg
961 atacaagaat tattgaagtt ctctgtaaag aacatgaatg taatatagat gaggtgaaaa
1021 atgtttattt caaaaatttt atacctttta tgaattctct tggacttgta acatctaatg
1081 gacttccaga ggttgaaaat ctttctaaac gatacgaaga aatttatctt aaaaataaag
1141 atctagatgc aagattattt ttggatcatg ataaaactct tcagactgat tctatagaca
1201 gttttgaaac acagagaaca ccacgaaaaa gtaaccttga tgaagaggtg aatgtaattc
1261 ctccacacac tccagttagg actgttatga acactatcca acaattaatg atgattttaa
1321 attcagcaag tgatcaacct tcagaaaatc tgatttccta ttttaacaac tgcacagtga
1381 atccaaaaga aagtatactg aaaagagtga aggatatagg atacatcttt aaagagaaat
1441 ttgctaaagc tgtgggacag ggttgtgtcg aaattggatc acagcgatac aaacttggag
1501 ttcgcttgta ttaccgagta atggaatcca tgcttaaatc agaagaagaa cgattatcca
1561 ttcaaaattt tagcaaactt ctgaatgaca acatttttca tatgtcttta ttggcgtgcg
1621 ctcttgaggt tgtaatggcc acatatagca gaagtacatc tcagaatctt gattctggaa
1681 cagatttgtc tttcccatgg attctgaatg tgcttaattt aaaagccttt gatttttaca
1741 aagtgatcga aagttttatc aaagcagaag gcaacttgac aagagaaatg ataaaacatt
1801 tagaacgatg tgaacatcga atcatggaat cccttgcatg gctctcagat tcacctttat
1861 ttgatcttat taaacaatca aaggaccgag aaggaccaac tgatcacctt gaatctgctt
1921 gtcctcttaa tcttcctctc cagaataatc acactgcagc agatatgtat ctttctcctg
1981 taagatctcc aaagaaaaaa ggttcaacta cgcgtgtaaa ttctactgca aatgcagaga
2041 cacaagcaac ctcagccttc cagacccaga agccattgaa atctacctct ctttcactgt
2101 tttataaaaa agtgtatcgg ctagcctatc tccggctaaa tacactttgt gaacgccttc
2161 tgtctgagca cccagaatta gaacatatca tctggaccct tttccagcac accctgcaga
2221 atgagtatga actcatgaga gacaggcatt tggaccaaat tatgatgtgt tccatgtatg
2281 gcatatgcaa agtgaagaat atagacctta aattcaaaat cattgtaaca gcatacaagg
2341 atcttcctca tgctgttcag gagacattca aacgtgtttt gatcaaagaa gaggagtatg
2401 attctattat agtattctat aactcggtct tcatgcagag actgaaaaca aatattttgc
2461 agtatgcttc caccaggccc cctaccttgt caccaatacc tcacattcct cgaagccctt
2521 acaagtttcc tagttcaccc ttacggattc ctggagggaa catctatatt tcacccctga
2581 agagtccata taaaatttca gaaggtctgc caacaccaac aaaaatgact ccaagatcaa
2641 gaatcttagt atcaattggt gaatcattcg ggacttctga gaagttccag aaaataaatc
2701 agatggtatg taacagcgac cgtgtgctca aaagaagtgc tgaaggaagc aaccctccta
2761 aaccactgaa aaaactacgc tttgatattg aaggatcaga tgaagcagat ggaagtaaac
2821 atctcccagg agagtccaaa tttcagcaga aactggcaga aatgacttct actcgaacac
2881 gaatgcaaaa gcagaaaatg aatgatagca tggatacctc aaacaaggaa gagaaatgag
2941 gatctcagga ccttggtgga cactgtgtac acctctggat tcattgtctc tcacagatgt
3001 gactgtataa ctttcccagg ttctgtttat ggccacattt aatatcttca gctctttttg
3061 tggatataaa atgtgcagat gcaattgttt gggtgattcc taagccactt gaaatgttag
3121 tcattgttat ttatacaaga ttgaaaatct tgtgtaaatc ctgccattta aaaagttgta
3181 gcagattgtt tcctcttcca aagtaaaatt gctgtgcttt atggatagta agaatggccc
3241 tagagtggga gtcctgataa cccaggcctg tctgactact ttgccttctt ttgtagcata
3301 taggtgatgt ttgctcttgt ttttattaat ttatatgtat atttttttaa tttaacatga
3361 acacccttag aaaatgtgtc ctatctatct tccaaatgca atttgattga ctgcccattc
3421 accaaaatta tcctgaactc ttctgcaaaa atggatatta ttagaaatta gaaaaaaatt
3481 actaatttta cacattagat tttattttac tattggaatc tgatatactg tgtgcttgtt
3541 ttataaaatt ttgcttttaa ttaaataaaa gctggaagca aagtataacc atatgatact
3601 atcatactac tgaaacagat ttcatacctc agaatgtaaa agaacttact gattattttc
3661 ttcatccaac ttatgttttt aaatgaggat tattgatagt actcttggtt tttataccat
3721 tcagatcact gaatttataa agtacccatc tagtacttga aaaagtaaag tgttctgcca
3781 gatcttaggt atagaggacc ctaacacagt atatcccaag tgcactttct aatgtttctg
3841 ggtcctgaag aattaagata caaattaatt ttactccata aacagactgt taattatagg
3901 agccttaatt tttttttcat agagatttgt ctaattgcat ctcaaaatta ttctgccctc
3961 cttaatttgg gaaggtttgt gttttctctg gaatggtaca tgtcttccat gtatcttttg
4021 aactggcaat tgtctattta tcttttattt tttttaagtc agtatggtct aacactggca
4081 tgttcaaagc cacattattt ctagtccaaa attacaagta atcaagggtc attatgggtt
4141 aggcattaat gtttctatct gattttgtgc aaaagcttca aattaaaaca gctgcattag
4201 aaaaagaggc gcttctcccc tcccctacac ctaaaggtgt atttaaacta tcttgtgtga
4261 ttaacttatt tagagatgct gtaacttaaa ataggggata tttaaggtag cttcagctag
4321 cttttaggaa aatcactttg tctaactcag aattattttt aaaaagaaat ctggtcttgt
4381 tagaaaacaa aattttattt tgtgctcatt taagtttcaa acttactatt ttgacagtta
4441 ttttgataac aatgacacta gaaaacttga ctccatttca tcattgtttc tgcatgaata
4501 tcatacaaat cagttagttt ttaggtcaag ggcttactat ttctgggtct tttgctacta
4561 agttcacatt agaattagtg ccagaatttt aggaacttca gagatcgtgt attgagattt
4621 cttaaataat gcttcagata ttattgcttt attgcttttt tgtattggtt aaaactgtac
4681 atttaaaatt gctatgttac tattttctac aattaatagt ttgtctattt taaaataaat
4741 tagttgttaa gagtcaaaaa aaaaaaaaaa aaaaaaaaaa a
//