LOCUS BC038960 2695 bp mRNA linear HUM 03-JAN-2005
DEFINITION Homo sapiens hect domain and RLD 3, mRNA (cDNA clone
IMAGE:6050308), complete cds.
ACCESSION BC038960
VERSION BC038960.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2695)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2695)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (01-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 82 Row: o Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7657151
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..2695
/db_xref="H-InvDB:HIT000052095"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6050308"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2695
/gene="HERC3"
/gene_synonym="KIAA0032"
/db_xref="GeneID:8916"
CDS 178..1284
/gene="HERC3"
/gene_synonym="KIAA0032"
/codon_start=1
/product="HERC3 protein"
/protein_id="AAH38960.1"
/db_xref="GeneID:8916"
/translation="MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGN
HSVFLLEDGEVYTCGLNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDR
GQLFSWGAGSDGQLGLMTTEDSVAVPRLIQKLNQQTILQVSCGNWHCLALAADGQFFT
WGKNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGAHSFALSLSGAVFGWGMNN
AGQLGLSDEKDRESPCHVKLLRTQKVVYISCGEEHTAVLTKSGGVFTFGAGSCGQLGH
DSMNDEVNPRRVLELMGSEVTQIACGRQHTLAFVPSSGLIYAFGCGARGQLGTGHTCN
VKCPSPVKGYWAAHSGQLSARAGKNDCLWNLKVF"
BASE COUNT 799 a 516 c 661 g 719 t
ORIGIN
1 ccacgcgtcc gcgaaaacgg agaaaccccg ggtccggcga gaggggctgt gacagtcgga
61 gtcccaagct gcggttcggc tgctgccgag aactgcaagg tgtggaatat ttctggcttc
121 tagtccaatg ccaagtgtgt gacctgtggc tacatgattc cctgaaagat aagaacaatg
181 ttatgttggg gatattggtc tctgggccaa cctggtatca gcaccaacct gcagggaatt
241 gtggctgagc cccaggtgtg tgggttcata tctgacagaa gtgtcaagga agtggcctgt
301 gggggaaacc actctgtgtt cctgctggaa gatggggaag tttacacatg tggtttgaac
361 accaaggggc aactgggcca tgagagggaa ggaaacaagc cagaacaaat tggagctctg
421 gcagatcagc atatcattca tgtggcatgt ggcgagtccc acagtctggc cctcagtgac
481 cgaggccagc tgttttcttg gggtgcaggg agtgatggtc agctaggact catgactact
541 gaggattctg tggcagtgcc caggttaata caaaagctga accagcaaac aatattacaa
601 gtttcctgtg gcaactggca ttgcttggct cttgcggctg atggccagtt cttcacctgg
661 ggaaagaaca gccatgggca gcttggctta gggaaggagt tcccctccca agccagccca
721 cagagggtga ggtccctgga ggggatccca ctggctcagg tggctgccgg aggggctcac
781 agctttgccc tgtctctctc aggagctgtt tttggctggg ggatgaataa tgccgggcag
841 ctagggctca gtgatgaaaa agatcgagaa tctccatgcc atgtaaaact cttacgcacg
901 caaaaagttg tctatattag ttgtggagaa gaacacacag cagttctcac aaagagtgga
961 ggtgtgttta cctttggcgc tggttcctgt gggcaacttg gacacgactc catgaatgat
1021 gaggttaacc ctagaagagt tctagagctg atgggtagtg aagtaactca aattgcttgt
1081 ggcagacaac ataccctagc cttcgtgcct tcttctggac tcatctatgc atttggttgt
1141 ggagcaagag gtcaattagg aactgggcac acttgtaatg ttaagtgccc atctcctgtc
1201 aagggttact gggctgccca cagtggccag ctttcagccc gagctggtaa gaatgattgt
1261 ctctggaatt taaaggtatt ttaagtgatg aaaacagttg tttacagaat atgtatctat
1321 tacttacttt gaggtctttt ggagggaatg aagataaggt attttttaac atcaattaat
1381 aatttctgta cttactatgc atgaagctgt agagttgaga tcctagagaa taaataaagc
1441 aggaaatact gttgtgttag gccgttcttg cattgctgta aagagcacct gaaacagggt
1501 gatttatgaa gaaaagagtt ttaattggct cacagttctg caggctctac agggagcatg
1561 atgcagccat ctgcttggct tctggggagg cctcaggaat cttacagtca tggcagaagg
1621 caaagaagga gcagacactt cacatggcag aagcaggagc aagaggaaga gtggagagga
1681 ggtgccatac tttttacacc accagatctc atgtgaactc agagcaagag ctcacttatc
1741 accaagggga tggcccaagc cattcatgag ggatccgccc tcatgatcta aacacatacc
1801 accatgcctc acctccaaca ttgggattac tgcaattcaa catgagtttt gaccagaaca
1861 tacattcaaa ctataccagt tattttgttt ccacagacct tggtggtggt gtatacattt
1921 tattgttgtt gctaataatg acagttcgca tgtattgaat gcttaatgac gtttcattac
1981 tatgcaaagc actcaaaata atctaagaac tagttagtgg ttattatccc catgttagag
2041 ataagggaac aaagtcttaa ggaggtcaag taatttgccc aagatcacat agattctgga
2101 ggcaggattt aaacccaggt cattctaaat catgtgtact atgttacctc tactttgctg
2161 ttggcatttt ggtatataac atgatgtgat ttctttttaa gccatttttt ttcttccctt
2221 aaaaaatagt taaatagcag tcttggtttc attggaaatc aaagtcaggc agtcattaat
2281 attgaatgac tgcttcacct tgaaataata aatctgaggc tgaaatggag ctagagaatc
2341 attaaaagga agtgtaactt attaattttg attttgaagt cgataaagtg acacacttat
2401 ggcttgatca ggaatttgtt tgaaacagta accatcataa gttttagtac tggattataa
2461 gaaactcaag gacacagaca aactcttttc ctttttgtaa acctagcata tagcacagga
2521 cctggcatga acttgtcact cagtagacat ttttggaatg gatatatgag catctgaaat
2581 gtagtgcaac ttaaaatata aataaacaag attttgtctg ggtgatgttc ccagaagcaa
2641 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa
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