LOCUS BC038452 1554 bp mRNA linear HUM 07-OCT-2003
DEFINITION Homo sapiens phospholipase A2, group VII (platelet-activating
factor acetylhydrolase, plasma), mRNA (cDNA clone MGC:46165
IMAGE:5216829), complete cds.
ACCESSION BC038452
VERSION BC038452.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1554)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1554)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (04-OCT-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 78 Row: g Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4826883.
FEATURES Location/Qualifiers
source 1..1554
/db_xref="H-InvDB:HIT000052039"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:46165 IMAGE:5216829"
/tissue_type="Blood, adult leukocytes"
/clone_lib="NIH_MGC_118"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1554
/gene="PLA2G7"
/gene_synonym="LDL-PLA2"
/gene_synonym="PAFAH"
/db_xref="GeneID:7941"
/db_xref="MIM:601690"
CDS 179..1504
/gene="PLA2G7"
/gene_synonym="LDL-PLA2"
/gene_synonym="PAFAH"
/codon_start=1
/product="PLA2G7 protein"
/protein_id="AAH38452.1"
/db_xref="GeneID:7941"
/db_xref="MIM:601690"
/translation="MVPPKLHVLFCLCGCLAVVYPFDWQYINPVAHMKSSAWVNKIQV
LMAAASFGQTKIPRGNGPYSVGCTDLMFDHTNKGTFLRLYYPSQDNDRLDTLWIPNKE
YFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRPGEKYPLVVFSHGLGAFRTLY
SAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIR
NEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGG
ATVIQTLSEDQRFRCGIALDAWMFPLGDEVYSRIPQPLFFINSEYFQYPANIIKMKKC
YSPDKERKMITIRGSVHQNFADFTFATGKIIGHMLKLKGDIDSNAAIDLSNKASLAFL
QKHLGLHKDFDQWDCLIEGDDENLIPGTNINTTNQHIMLQNSSGIEKYN"
misc_feature 320..1426
/gene="PLA2G7"
/gene_synonym="LDL-PLA2"
/gene_synonym="PAFAH"
/note="PAF-AH_p_II; Region: Platelet-activating factor
acetylhydrolase, plasma/intracellular isoform II.
Platelet-activating factor acetylhydrolase (PAF-AH) is a
subfamily of phospholipases A2, responsible for
inactivation of platelet-activating factor through
cleavage of an acetyl group. Three known PAF-AHs are the
brain heterotrimeric PAF-AH Ib, whose catalytic beta and
gamma subunits are aligned in pfam02266, the
extracellular, plasma PAF-AH (pPAF-AH), and the
intracellular PAF-AH isoform II (PAF-AH II). This family
aligns pPAF-AH and PAF-AH II, whose similarity was
previously noted"
/db_xref="CDD:pfam03403"
BASE COUNT 487 a 311 c 331 g 425 t
ORIGIN
1 ggaaaaccgg cctgactggg gggtgaattc agcagggagt aaatctgatc ggcatcaggt
61 ctgcggaaag gagctggtga gcacgacacc acccaggcat tgcctggctc tctccgcggc
121 gggctaagtt aaccgcgggt ccaggagact aagctgaaac tgctgctcag ctcccaagat
181 ggtgccaccc aaattgcatg tgcttttctg cctctgcggc tgcctggctg tggtttatcc
241 ttttgactgg caatacataa atcctgttgc ccatatgaaa tcatcagcat gggtcaacaa
301 aatacaagta ctgatggctg ctgcaagctt tggccaaact aaaatccccc ggggaaatgg
361 gccttattcc gttggttgta cagacttaat gtttgatcac actaataagg gcaccttctt
421 gcgtttatat tatccatccc aagataatga tcgccttgac accctttgga tcccaaataa
481 agaatatttt tggggtctta gcaaatttct tggaacacac tggcttatgg gcaacatttt
541 gaggttactc tttggttcaa tgacaactcc tgcaaactgg aattcccctc tgaggcctgg
601 tgaaaaatat ccacttgttg ttttttctca tggtcttggg gcattcagga cactttattc
661 tgctattggc attgacctgg catctcatgg gtttatagtt gctgctgtag aacacagaga
721 tagatctgca tctgcaactt actatttcaa ggaccaatct gctgcagaaa taggggacaa
781 gtcttggctc taccttagaa ccctgaaaca agaggaggag acacatatac gaaatgagca
841 ggtacggcaa agagcaaaag aatgttccca agctctcagt ctgattcttg acattgatca
901 tggaaagcca gtgaagaatg cattagattt aaagtttgat atggaacaac tgaaggactc
961 tattgatagg gaaaaaatag cagtaattgg acattctttt ggtggagcaa cggttattca
1021 gactcttagt gaagatcaga gattcagatg tggtattgcc ctggatgcat ggatgtttcc
1081 actgggtgat gaagtatatt ccagaattcc tcagcccctc ttttttatca actctgaata
1141 tttccaatat cctgctaata tcataaaaat gaaaaaatgc tactcacctg ataaagaaag
1201 aaagatgatt acaatcaggg gttcagtcca ccagaatttt gctgacttca cttttgcaac
1261 tggcaaaata attggacaca tgctcaaatt aaagggagac atagattcaa atgcagctat
1321 tgatcttagc aacaaagctt cattagcatt cttacaaaag catttaggac ttcataaaga
1381 ttttgatcag tgggactgct tgattgaagg agatgatgag aatcttattc cagggaccaa
1441 cattaacaca accaatcaac acatcatgtt acagaactct tcaggaatag agaaatacaa
1501 ttaggattaa aataggtttt ttaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//