LOCUS BC038419 1710 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and
steroid delta-isomerase 2, mRNA (cDNA clone MGC:34587
IMAGE:5167092), complete cds.
ACCESSION BC038419
VERSION BC038419.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1710)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1710)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-OCT-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 52 Row: o Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 50726973.
FEATURES Location/Qualifiers
source 1..1710
/db_xref="H-InvDB:HIT000052031"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:34587 IMAGE:5167092"
/tissue_type="Brain, adult medulla"
/clone_lib="NIH_MGC_119"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1710
/gene="HSD3B2"
/gene_synonym="HSDB"
/gene_synonym="HSDB3"
/db_xref="GeneID:3284"
/db_xref="HGNC:HGNC:5218"
/db_xref="MIM:201810"
CDS 148..1266
/gene="HSD3B2"
/gene_synonym="HSDB"
/gene_synonym="HSDB3"
/codon_start=1
/product="hydroxy-delta-5-steroid dehydrogenase, 3 beta-
and steroid delta-isomerase 2"
/protein_id="AAH38419.1"
/db_xref="GeneID:3284"
/db_xref="HGNC:HGNC:5218"
/db_xref="MIM:201810"
/translation="MGWSCLVTGAGGLLGQRIVRLLVEEKELKEIRALDKAFRPELRE
EFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVK
GTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPTPYPYSKKL
AEKAVLAANGWNLKNGDTLYTCALRPTYIYGEGGPFLSASINEALNNNGILSSVGKFS
TVNPVYVGNVAWAHILALRALRDPKKAPSVRGQFYYISDDTPHQSYDNLNYILSKEFG
LRLDSRWSLPLTLMYWIGFLLEVVSFLLSPIYSYQPPFNRHTVTLSNSVFTFSYKKAQ
RDLAYKPLYSWEEAKQKTVEWVGSLVDRHKETLKSKTQ"
BASE COUNT 477 a 426 c 383 g 424 t
ORIGIN
1 gggatgaggc agtaaggact tggactcctc tgtccagctt ttaacaatct aagttacgcc
61 ctcttctggg tcacgctaga atcagatctg ctctccagca tcttctgttt cctggcaagt
121 gtttcctgct actttggatt ggccacgatg ggctggagct gccttgtgac aggagcagga
181 gggcttctgg gtcagaggat cgtccgcctg ttggtggaag agaaggaact gaaggagatc
241 agggccttgg acaaggcctt cagaccagaa ttgagagagg aattttctaa gctccagaac
301 aggaccaagc tgactgtact tgaaggagac attctggatg agccattcct gaaaagagcc
361 tgccaggacg tctcggtcgt catccacacc gcctgtatca ttgatgtctt tggtgtcact
421 cacagagagt ccatcatgaa tgtcaatgtg aaaggtaccc agctactgtt ggaggcctgt
481 gtccaagcca gtgtgccagt cttcatctac accagtagca tagaggtagc cgggcccaac
541 tcctacaagg aaatcatcca gaacggccac gaagaagagc ctctggaaaa cacatggccc
601 actccatacc cgtacagcaa aaagcttgct gagaaggctg tgctggcggc taatgggtgg
661 aatctaaaaa atggtgatac cttgtacact tgtgcgttaa gacccacata tatctatggg
721 gaaggaggcc cattcctttc tgccagtata aatgaggccc tgaacaacaa tgggatcctg
781 tcaagtgttg gaaagttctc tacagtcaac ccagtctatg ttggcaacgt ggcctgggcc
841 cacattctgg ccttgagggc tctgcgggac cccaagaagg ccccaagtgt ccgaggtcaa
901 ttctattaca tctcagatga cacgcctcac caaagctatg ataaccttaa ttacatcctg
961 agcaaagagt ttggcctccg ccttgattcc agatggagcc ttcctttaac cctgatgtac
1021 tggattggct tcctgctgga agtagtgagc ttcctactca gcccaattta ctcctatcaa
1081 ccccccttca accgccacac agtcacatta tcaaatagtg tgttcacctt ctcttacaag
1141 aaggctcagc gagatctggc gtataagcca ctctacagct gggaggaagc caagcagaaa
1201 accgtggagt gggttggttc ccttgtggac cggcacaagg agaccctgaa gtccaagact
1261 cagtgattta aggatgacag agatgtgcat gtgggtattg ttaggaaatg tcatcaaact
1321 ccacccacct ggcttcatac agaaggcaac aggggcacaa gcccaggtcc tgctgcctct
1381 ctttcacaca atgcccaact tactgtcttc ttcatgtcat caaaatctgc acagtcactg
1441 gcccaaccag aactttctgt cctaatcata caccagaaga caaacaatat gatttgctgt
1501 taccaaatct cagtggctga ttctgaacaa ttgtggtctc tcttaacttg aggttctctt
1561 ttgactaata gagctccatt tcccctctta aatgagaaag catttctttt ctctttaatc
1621 tcctattcct tcacacagtt caacataaag agcaataaat gttttaatgc ttaaaaaaaa
1681 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
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