LOCUS BC038296 4578 bp mRNA linear HUM 24-JUL-2006
DEFINITION Homo sapiens Smg-5 homolog, nonsense mediated mRNA decay factor (C.
elegans), mRNA (cDNA clone MGC:33341 IMAGE:4824487), complete cds.
ACCESSION BC038296
VERSION BC038296.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4578)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4578)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-OCT-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 46 Row: m Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 24308040.
FEATURES Location/Qualifiers
source 1..4578
/db_xref="H-InvDB:HIT000052017"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:33341 IMAGE:4824487"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4578
/gene="SMG5"
/gene_synonym="EST1B"
/gene_synonym="KIAA1089"
/gene_synonym="LPTS-RP1"
/gene_synonym="LPTSRP1"
/gene_synonym="RP11-54H19.7"
/gene_synonym="SMG-5"
/db_xref="GeneID:23381"
/db_xref="HGNC:HGNC:24644"
CDS 151..3201
/gene="SMG5"
/gene_synonym="EST1B"
/gene_synonym="KIAA1089"
/gene_synonym="LPTS-RP1"
/gene_synonym="LPTSRP1"
/gene_synonym="RP11-54H19.7"
/gene_synonym="SMG-5"
/codon_start=1
/product="Smg-5 homolog, nonsense mediated mRNA decay
factor (C. elegans)"
/protein_id="AAH38296.1"
/db_xref="GeneID:23381"
/db_xref="HGNC:HGNC:24644"
/translation="MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAY
QEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIH
SRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSAS
GKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQL
GTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSP
GKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLS
LASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLV
NHVNIRLQAELEEGENPVPAFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKS
RKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAF
DAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASN
LQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGS
ESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSL
WNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPL
RAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQ
SEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPY
LVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFK
KGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTI
ITGLPLDNPSVLSGPMQAALQAAAHASVDIKDVLDFYKQWKEIG"
BASE COUNT 1019 a 1308 c 1321 g 930 t
ORIGIN
1 agcgggtcgt gggcagccgc ctcacagcga tggcggccga gcagggccgg tggcggcggc
61 ggctgcggct acggccggag acggcagtgt tggcggtagt ggtgggtggc aggggcctgt
121 gaccgggagc tgcccccgga cccgggcacc atgagccaag gcccccccac aggggagagc
181 agcgagcccg aagcaaaagt cctccacact aagcggcttt accgggctgt ggtggaggct
241 gtgcatcgac ttgacctcat cctttgcaac aaaactgctt atcaagaagt attcaaacca
301 gaaaacatta gcctgaggaa caagctgcgt gagctctgcg tcaagcttat gttcctgcac
361 ccagtggact atgggagaaa ggctgaggag ctgctgtgga gaaaggtata ctatgaagtt
421 atccagctta tcaagactaa caaaaagcac atccacagcc ggagcacttt ggaatgtgcc
481 tacaggacgc acctggttgc tggtattggc ttctaccagc atctccttct ctatatccag
541 tcccactacc agctggaact gcagtgctgc atcgactgga cccatgtcac tgaccccctc
601 ataggatgca agaagccagt gtctgcctca gggaaggaga tggattgggc acagatggca
661 tgtcaccgat gtctggtgta tctgggggat ttgtcccgat atcagaatga attagctggc
721 gtagataccg agctgctagc cgagagattt tactaccaag ccctgtcagt agctcctcag
781 attggaatgc ccttcaatca gctgggcacc ctggcaggca gcaagtacta taatgtggaa
841 gccatgtatt gctacctgcg ctgcatccag tcagaagtgt cctttgaggg agcctatggg
901 aacctcaagc ggctgtatga caaggcagcc aaaatgtacc accaactgaa gaagtgtgag
961 actcggaaac tgtctcctgg caaaaagcga tgtaaagaca ttaaaaggtt gctagtgaac
1021 tttatgtatc tgcaaagcct cctacagccc aaaagcagct ccgtggactc agagctgacc
1081 tcactttgcc agtcagtcct ggaggacttc aacctctgcc tcttctacct gccctcctca
1141 cccaacctca gcctggccag tgaggatgag gaggagtatg agagtggata tgctttcctc
1201 ccggaccttc tcatctttca aatggtcatc atctgcctta tgtgtgtgca cagcttggag
1261 agagcaggat ccaagcagta cagtgcagcc attgccttca ccctggccct cttttcccac
1321 ctcgtcaatc atgtcaacat acggctgcag gctgagctgg aagagggcga gaatcccgtc
1381 ccggcattcc agagtgatgg cacagatgaa ccagagtcca aggaacctgt ggagaaagag
1441 gaggagccag atcctgagcc tcctcctgta acaccccaag tgggtgaggg cagaaagagc
1501 cgtaagttct ctcgcctctc ctgtctccgc cgtcgccgcc acccacccaa agttggtgat
1561 gacagtgacc tgagtgaagg ctttgaatcg gactcaagcc atgactcagc ccgggccagt
1621 gagggctcag acagtggctc tgacaagagt cttgaaggtg ggggaacggc ctttgatgct
1681 gaaacagact cggaaatgaa tagccaggag tcccgatcag acttggaaga tatggaggaa
1741 gaggagggga cacggtcacc aaccctggag ccccctcggg gcagatcaga ggctcccgat
1801 tccctcaatg gcccactggg ccccagtgag gctagcattg ccagcaatct acaagccatg
1861 tccacccaga tgttccagac taagcgctgc ttccgactgg cccccacctt tagcaacctg
1921 ctcctccagc ccaccaccaa ccctcatacc tcggccagcc acaggccttg cgtcaatggg
1981 gatgtagaca agccttcaga gccagcctct gaggagggct ctgagtcgga ggggagtgag
2041 tccagtggac gctcctgtcg gaatgagcgc agcatccagg agaagcttca ggtcctgatg
2101 gccgaaggtc tgcttcctgc tgtgaaagtc ttcctggact ggcttcggac caaccccgac
2161 ctcatcatcg tgtgtgcgca gagctctcaa agtctgtgga accgcctgtc tgtgttgctg
2221 aatctgttgc ctgctgctgg tgaactccag gagtctggcc tggccttgtg tcctgaggtc
2281 caagatcttc ttgaaggttg tgaactgcct gacctcccct ctagccttct gctcccagag
2341 gacatggctc ttcgtaacct gcccccgctc cgagctgccc acagacgctt taactttgac
2401 acggatcggc ccctgctcag caccttagag gagtcagtgg tgcgcatctg ctgcatccgc
2461 agctttggtc atttcatcgc ccgcctgcaa ggcagcatcc tgcagttcaa cccagaggtt
2521 ggcatcttcg tcagcattgc ccagtctgag caggagagcc tgctgcagca ggcccaggca
2581 cagttccgaa tggcacagga ggaagctcgt cggaacaggc tcatgagaga catggctcag
2641 ctacgacttc agctcgaagt gtctcagctg gagggcagcc tgcagcagcc caaggcccag
2701 tcagccatgt ctccctacct cgtccctgac acccaggccc tctgccacca tctccctgtc
2761 atccgccaac tggccaccag tggccgcttc attgtcatca tcccaaggac agtgatcgat
2821 ggcctggatt tgctgaagaa ggaacaccca ggggcccggg atgggattcg gtacctggag
2881 gcagagttta aaaaaggaaa caggtacatt cgctgccaga aagaggtggg aaagagcttt
2941 gagcggcata agctgaagag gcaggatgca gatgcctgga ctctctataa gatcctagac
3001 agctgcaaac agctgactct ggcccagggg gcaggtgagg aggatccgag tggcatggtg
3061 accatcatca caggccttcc actggacaac cccagcgtgc tttcaggccc catgcaggca
3121 gccctgcagg ccgctgccca cgccagtgtg gacatcaagg atgttctgga cttctacaag
3181 cagtggaagg aaattggttg atactgaccc ccaggccctg cagtggggct gactccagat
3241 ctctcctgcc ctccctggca gccaggacca gcacctgtag tcaccccacc acacgcagac
3301 tcatgcacgc acacaggagg gaggcctagc tgctcagagg ctgcagggag ggcccaggag
3361 ccggctggga gggtggggtc cctttgttgc caagacgtta ggaaagcgag gaaagtgctt
3421 ggattaggag agtcttgtgg gcccctggcc agccttcctg cctcagctcc cctgctgtct
3481 ccaggggcag gtggtaggca tgggtacctg catttcactg gaatgggttc ttggatctct
3541 gaggggaagg aacagcaaaa gaggcccttc ttcctcaccc aagatgcagg gtggttgggg
3601 ccaggagttt ggaccctcta ggtcttgggg gaagagctgg gtaatacctg gtgtctgagt
3661 gattctctgc agacccttcc cctcctcaag gatcacccat cctcctttca gcccccttta
3721 tggggaccag gcagctctgg agccagccac aggggctgtt agagaagcaa ggcctggagt
3781 ggcctgcacc gagtagcagg gtcagggttc gtgtgctcct cctcctgctg caggggctgc
3841 acatcccatt gccccacttc tgctttgtgt ctccctctgt ctagcttcca gggcagggag
3901 caggccccac ctagggctgc aggcagtctg gcctgtgcca gcacggtctc ctgtgcccac
3961 cagccccaca ggtgctgtgc tttgtgctct tggctgctgt gctgggacag aatgggatgc
4021 caggaagaga agaaaggggg tgcagtctga ggccaccacc ccccttccta tctaagggag
4081 ggctgaagac aaggggccgg cattcagtgg gcagcagaaa ggagaggctc cttgaagctg
4141 ctcagtcaga ggcccccgtc cctccttttg ccttccgcag gactgaagac ctgaaggggc
4201 tggcttttgg agtgttgagg tgaatatctg ggagcagaga tcatgaatag ctcagggcag
4261 tgaatggcgc accaagagca gggctgtgtg tgggaggctg cagccaggat tgcctcagct
4321 cctccccctc aggctgggag gatagcacag gctaggggct cggggtggag ggtctcagct
4381 ctgctgcccc caccccagta ctagcctagc ttcccaagct gtggcttaga ggatagttgg
4441 cttcctgcct ctctcctcta aaatagcaag tctgggaaat cctggggtga gtggagtcac
4501 cccactccca gttgctggca gagactgaga ctaaagcatc acttaataaa ccccccaagc
4561 ccaaaaaaaa aaaaaaaa
//