LOCUS BC037303 5083 bp mRNA linear HUM 19-JUN-2006
DEFINITION Homo sapiens protein kinase, AMP-activated, alpha 1 catalytic
subunit, mRNA (cDNA clone MGC:33776 IMAGE:5265054), complete cds.
ACCESSION BC037303
VERSION BC037303.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5083)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5083)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-SEP-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Sep 16, 2003 this sequence version replaced BC037303.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 47 Row: i Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 47458812.
FEATURES Location/Qualifiers
source 1..5083
/db_xref="H-InvDB:HIT000051900"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:33776 IMAGE:5265054"
/tissue_type="Brain, hippocampus"
/clone_lib="NIH_MGC_95"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..5083
/gene="PRKAA1"
/gene_synonym="1"
/gene_synonym="alpha"
/gene_synonym="AMPK"
/gene_synonym="AMPKa1"
/gene_synonym="MGC33776"
/gene_synonym="MGC57364"
/db_xref="GeneID:5562"
/db_xref="HGNC:HGNC:9376"
/db_xref="MIM:602739"
CDS 16..1668
/gene="PRKAA1"
/gene_synonym="1"
/gene_synonym="alpha"
/gene_synonym="AMPK"
/gene_synonym="AMPKa1"
/gene_synonym="MGC33776"
/gene_synonym="MGC57364"
/codon_start=1
/product="PRKAA1 protein"
/protein_id="AAH37303.1"
/db_xref="GeneID:5562"
/db_xref="HGNC:HGNC:9376"
/db_xref="MIM:602739"
/translation="MATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKV
AVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGE
LFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADF
GLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDD
DHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPK
YLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRR
IMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQG
VRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMS
LQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRSCQRSDSDAEAQGKSS
EVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMCANLIKILAQ"
BASE COUNT 1572 a 870 c 913 g 1728 t
ORIGIN
1 agttcctgga gaaagatggc gacagccgag aagcagaaac acgacgggcg ggtgaagatc
61 ggccactaca ttctgggtga cacgctgggg gtcggcacct tcggcaaagt gaaggttggc
121 aaacatgaat tgactgggca taaagtagct gtgaagatac tcaatcgaca gaagattcgg
181 agccttgatg tggtaggaaa aatccgcaga gaaattcaga acctcaagct tttcaggcat
241 cctcatataa ttaaactgta ccaggtcatc agtacaccat ctgatatttt catggtgatg
301 gaatatgtct caggaggaga gctatttgat tatatctgta agaatggaag gctggatgaa
361 aaagaaagtc ggcgtctgtt ccaacagatc ctttctggtg tggattattg tcacaggcat
421 atggtggtcc atagagattt gaaacctgaa aatgtcctgc ttgatgcaca catgaatgca
481 aagatagctg attttggtct ttcaaacatg atgtcagatg gtgaattttt aagaacaagt
541 tgtggctcac ccaactatgc tgcaccagaa gtaatttcag gaagattgta tgcaggccca
601 gaggtagata tatggagcag tggggttatt ctctatgctt tattatgtgg aacccttcca
661 tttgatgatg accatgtgcc aactcttttt aagaagatat gtgatgggat cttctatacc
721 cctcaatatt taaatccttc tgtgattagc cttttgaaac atatgctgca ggtggatccc
781 atgaagaggg ccacaatcaa agatatcagg gaacatgaat ggtttaaaca ggaccttcca
841 aaatatctct ttcctgagga tccatcatat agttcaacca tgattgatga tgaagcctta
901 aaagaagtat gtgaaaagtt tgagtgctca gaagaggaag ttctcagctg tctttacaac
961 agaaatcacc aggatccttt ggcagttgcc taccatctca taatagataa caggagaata
1021 atgaatgaag ccaaagattt ctatttggcg acaagcccac ctgattcttt tcttgatgat
1081 catcacctga ctcggcccca tcctgaaaga gtaccattct tggttgctga aacaccaagg
1141 gcacgccata cccttgatga attaaatcca cagaaatcca aacaccaagg tgtaaggaaa
1201 gcaaaatggc atttaggaat tagaagtcaa agtcgaccaa atgatattat ggcagaagta
1261 tgtagagcaa tcaaacaatt ggattatgaa tggaaggttg taaacccata ttatttgcgt
1321 gtacgaagga agaatcctgt gacaagcact tactccaaaa tgagtctaca gttataccaa
1381 gtggatagta gaacttatct actggatttc cgtagtattg atgatgaaat tacagaagcc
1441 aaatcaggga ctgctactcc acagagatcg ggatcagtta gcaactatcg atcttgccaa
1501 aggagtgatt cagatgctga ggctcaagga aaatcctcag aagtttctct tacctcatct
1561 gtgacctcac ttgactcttc tcctgttgac ctaactccaa gacctggaag tcacacaata
1621 gaattttttg agatgtgtgc aaatctaatt aaaattcttg cacaataaac agaaaacttt
1681 gcttatttct tttgcagcaa taagcatgca taataagtca cagccaaatg cttccatttg
1741 taatcaagtt atacataatt ataaccgagg gctggcgttt tggaatgcaa tttgcacagg
1801 gattggaaca tgatttatag ttaaaagcct aatatgcaga aatgaattaa gatcattttg
1861 ttgttcattg tgcagtatgt atatagcata atatacacag tgaattatag gtctcaggct
1921 tacttgattt ttggctattt tatatttagt gtacacaggg ctttgaaata ttaatttaca
1981 taaaggcctt catatattat tacgtgttat atattacgtg ttataaattt attcaataaa
2041 tatttgccta gaattcccaa gacctttata ggtgattttg ttttctgggc tccttaactt
2101 cataaatagc tagtatcttc cagcagtagt aacagtctgg ataacttctt ccatatccct
2161 ccctctttgt ttttttgaga cagtgtcact ttgtcaccca ggctggagtg caatggtgtg
2221 gtctcggctc actgcaacct ccacctcccg ggttcgagtg attctcccgc ctcagcttcc
2281 tgagtagctg gaactacagg cgtgtgccac cacacccggc taatttttcg tatttttagt
2341 gtagacgggg tttcactatg ttgcccaggc tggtctcgaa ctcctgaccg cgtgatccac
2401 cacctcagct tcccaaagtg gtgggattac aggcgtgagc caccgcaccc ggcctccata
2461 tccccctttt aaaattctgt agtgtatggt aagtcatatc agatatcaga cctaatttaa
2521 atttcatttt agctttacaa gtccaaaaac acagaattta tatattcaga tactctagca
2581 ctaattttag tcttaaaata ttcccacgat attctgtaca caaaatgttc tttttgttac
2641 aagagctgag ttgcatatac tgtagataaa tcatattatt tttgccaatt tcacaaattc
2701 ctctggccca tcatgtcagt cattattgag tatatgcaca cattgctact tatttgatta
2761 tgtatctttt aaattgattc agtgcataga aaactatctc ttacaaactt taagtgctct
2821 gatatgactt cccccccaaa ttttattatg aacattttta aaaacagaaa aattgaaaaa
2881 ctgtttggta agcacatgta tatctaccat ttagattcag cagttgttaa tgttttgtca
2941 tttgttttct ctatacctat atatgtatag atacagctag ttatgcatat atatgcatat
3001 atgtgtttgt ttgtgtatgt atatatgctt ttttccccct gaaccatttg gatgttacag
3061 acatacttat caccgtgaaa atacttcaag tatctcctac agataatgac attctcctaa
3121 aaatccgtaa taccattgta aaagtaataa ttccccaata tcatctaatc aagccatatt
3181 taaatttctg aagttaactc caaatttctt tatagctgat tatttcaaac taggatccaa
3241 ttaaagttta catatgacac ttggttataa ctctttagtt ggatataaca ttattattat
3301 tttgataaaa tatggaacaa atcaattcta ttaataagtg gtcacatttg ttttgggctt
3361 aaattacttt ttaaagatac tggattttcc taagatttct gatttacact gatatttttt
3421 tttgtcattc ttaattgcat cacacaatag atgtaaatga agatgtagtc acctcagata
3481 aaattggtat cgtgtatgat aatattgtat catttatatt tgccttatgt taactttaag
3541 aaattgattt ttttgtatta atcattttcc cattgcaaca gagctatatt ttttctattt
3601 taagaatcat attttaggat tatttttggc aaatacagtg agcacttatg taaccagatg
3661 ataatgaact caaatgtcat gatagcttgc ataaatggtg actctagtag atttgactca
3721 agcacttcta gaatcatgca ctgaattcaa aggaaaaatc ttgctgcttt ttgtccaggg
3781 cttgttctat tcaacttcta atttgaaagc tgtacaaagt aatagaagtt ccatttaaat
3841 atgagttcaa aactgtattt actttttatg tggccctctc tttaggggat tctaatttta
3901 cttagggtct ctaagtgcag cataatgttc ctgatgttaa cagaagactg tatttttaaa
3961 gttacaaatt tgtatatgga attaagtaat ggcgctatat acgctgttgt ggggaggggg
4021 gaagaaaagg aggaaccaat taaataggac cttttaaaaa ttgttaattt tgtaaacttt
4081 gcttctctta taagttattg tgattcattt tagttactgt gttttatttt gaaaatattt
4141 aaatattgca cttctataaa tagtatgata aatgcacaga caattgcagt aaattctttt
4201 ttaagctagg atatttgaaa tgacaacctt tggttaagtg tgtcaaggtt gcaacagaat
4261 tttcacaatt tttttgttgt ttgcaaattg ttactaatat tgaagaggta agggaggcaa
4321 tgcaaatgat ttttaatctt tttttattat cttttcagca gtttatattt tttgtgactt
4381 tatgcaacca tatttttact ttgtcttgac aactgaaaga tgtataaggt tttttgccag
4441 aaatgtactg tatacatagt tttaagtata acagatttta ctgatatgta aaaattttgc
4501 cattaaaata aatgatttct cactgagagg aacttttcta ccaggttggg gcatatggga
4561 gcttaatata tcatatctaa tttaaaataa tttcactgaa ataaactcca ttgcttttac
4621 ctaatttttt tcttgagatg cttttgtagt ttttcagagt tttagatgat tttatacaaa
4681 atcctctgcc tagcactgct ctttttgatg ttgtagtgac accatttaca ttgaattaat
4741 gcttggtagc ctggggctag atgtggaact ccatggatct gtgttctgac tggcaccttt
4801 ggaatgaaag aaaagtgtgt gctgtccaaa ttttttcccc ttaattcttt ccctcatctt
4861 ctcacccata atagaaattt tatttccatt gtgagttctg acaagaatga aattccacat
4921 acaacataac tgtaaattgt tggtaggtag aagttaatat ttgtggttca tgtatatttt
4981 gaccagagta tatttaagta tataatttca gcttccttga tttagaaata tgatataata
5041 aagaaaaact ccatttatca tctgttaaaa aaaaaaaaaa aaa
//