LOCUS BC036809 3483 bp mRNA linear HUM 19-APR-2005
DEFINITION Homo sapiens Rho guanine nucleotide exchange factor (GEF) 10, mRNA
(cDNA clone IMAGE:5737598), complete cds.
ACCESSION BC036809
VERSION BC036809.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3483)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3483)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 79 Row: j Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7662041
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..3483
/db_xref="H-InvDB:HIT000051821"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5737598"
/tissue_type="Duodenum, adenocarcinoma"
/clone_lib="NIH_MGC_88"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3483
/gene="ARHGEF10"
/gene_synonym="GEF10"
/db_xref="GeneID:9639"
/db_xref="MIM:608136"
CDS 522..2504
/gene="ARHGEF10"
/gene_synonym="GEF10"
/codon_start=1
/product="ARHGEF10 protein"
/protein_id="AAH36809.1"
/db_xref="GeneID:9639"
/db_xref="MIM:608136"
/translation="MHSDEMIYDDVENGDEGGNSSLEYGWSSSEFESYEEQSDSECKN
GIPRSFLRSNHKKQMQKLVKAAKDGTKDGLERTRAAVKRGRSFIRTKSLIAQDHRSSL
EEEQNLFIDVDCKHPEAILTPMPEGLSQQQVVRRYILGSVVDSEKNYVDALKRILEQY
EKPLSEMEPKVLSERKLKTVFYRVKEILQCHSLFQIALASRVSEWDSVEMIGDVFVAS
FSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKQEQEASPDRTTLYSLMMKP
IQRFPQFILLLQDMLKNTSKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEVKQI
AKAINERYLNKLLSSGSRYLIRSDDMIETVYNDRGEIVKTKERRVFMLNDVLMCATVS
SRPSHDSRVMSSQRYLLKWSVPLGHVDAIEYGSSAGTGEHSRHLAVHPPESLAVVANA
KPNKVYMGPGQLYQDLQNLLHDLNVIGQITQLIGNLKGNYQNLNQSVAHDWTSGLQRL
ILKKEDEIRAADCCRIQLQLPGKQDKSGRPTFFTAVFNTFTPAIKESWVNSLQMAKLA
LEENHMGWFCVEDDGNHIKKEKHPLLVGHMPVMVAKQQEFKIECAAYNPEPYLNNESQ
PDSFSTAHGFLWVRCVYLVLVQVHRESTFMVGVMRD"
BASE COUNT 970 a 815 c 833 g 865 t
ORIGIN
1 ccacgcgtcc ggaaaattta aaaatccgtg tgtattttct tgctagtgct ggtacttgaa
61 tatctgtatc atccacctat ccatccacct acccatattt ctataatcca ccgtccatcg
121 acatgcctgt catctgtcca ccatttctct ctgtctaatt ttcaaaacat cctgtaagtt
181 tatataaagg aagatttttc ttcttgtgaa gttctctaag gctgacaagt tacctggcat
241 gactgtggcg gatgcccata gccaggtggt cctcggggta cagatggggc aggggcactt
301 gtgagaaaca cctgaagtgc ttttccccag cctccccggc cctgcccggg tggtggaggc
361 gctgcacggt gccttccatg gagcaagccc ggggctccgc agggtcctca gcatgattca
421 gatttccttc cacccccagc tctagatgat ttggtaaaac cacaaacagg cacaaaacag
481 cccacatgga attctaaagt tttaatttca ttttggaatt tatgcactca gatgaaatga
541 tttatgatga tgttgagaat ggggatgaag gtggaaacag ctccttggaa tacggatgga
601 gttcgagtga atttgaaagt tacgaagagc agagtgactc ggagtgcaag aatgggattc
661 ccaggtcctt cctgcgcagc aaccacaaaa agcaaatgca gaagctcgtg aaggccgcga
721 aggacggcac caaggacggg ctggagagga ccagggcagc cgtgaagagg ggccgctcct
781 tcatcaggac caagtctctc atcgcacagg atcacagatc ttctcttgag gaagaacaga
841 atttgttcat tgatgttgac tgcaagcacc cggaagccat cttgaccccg atgcccgagg
901 gtttatctca gcagcaggtt gtaagaagat atatactggg ttcagttgtc gacagtgaaa
961 agaactacgt agatgctctt aagaggattt tggagcaata tgagaagccg ctgtctgaga
1021 tggagccaaa ggttctgagt gagaggaagc tgaagacggt gttctaccga gtcaaagaga
1081 tcctgcagtg ccactcgcta tttcagatcg cgctggccag ccgcgtttcc gagtgggact
1141 ccgtggaaat gataggcgat gtcttcgtgg cttcgttttc taagtccatg gtgctggatg
1201 catacagtga atatgtgaac aatttcagca cagccgtggc agtcctcaag aaaacatgtg
1261 ccacaaagcc cgcttttctt gaatttttaa agcaggaaca ggaggccagc cccgatcgaa
1321 ccacgctcta cagcctgatg atgaagccca tccagaggtt cccacagttc atcctcctgc
1381 tccaggacat gctgaagaac acctccaaag gccaccccga caggctgcct cttcagatgg
1441 ccctgacaga gctcgaaaca ctagcagaga agttaaatga aagaaagaga gatgctgatc
1501 aacgctgtga agtgaagcaa atagccaaag ccataaacga aagatacctg aacaagcttc
1561 tcagcagtgg aagccgatac ctcattcgat cagatgatat gatagaaaca gtttacaacg
1621 acagaggaga gattgttaaa accaaagaac gccgagtctt catgttaaat gatgtgttaa
1681 tgtgtgccac cgtcagctca cgcccctctc atgacagccg tgtgatgagc agccagaggt
1741 acttgctgaa gtggagcgtt ccactgggac atgtggacgc catcgagtat ggcagcagcg
1801 caggcacggg cgagcacagc aggcaccttg ccgttcaccc gccggagagc ctggccgtgg
1861 ttgctaacgc gaaaccaaac aaagtttaca tggggccagg acaactgtat caagatttac
1921 aaaacttgtt gcatgactta aatgtaattg gccaaatcac tcagctgata ggaaacctta
1981 aaggaaacta tcagaactta aaccagtcag tagcccatga ctggacatca ggtttacaaa
2041 ggcttatttt gaagaaagaa gatgaaatca gagctgcgga ctgctgcaga attcagttac
2101 agcttcccgg gaagcaggac aaatctgggc gaccgacgtt ctttacagct gtgttcaata
2161 cgttcacccc tgccatcaag gagtcctggg tcaacagctt acagatggcc aagctcgccc
2221 tagaggagaa ccacatgggc tggttctgtg tggaagacga tgggaatcac attaaaaagg
2281 agaagcatcc tctcctcgtc ggacacatgc ccgtgatggt ggccaagcag caggagttca
2341 agattgaatg tgctgcttat aaccctgaac cttacctaaa taatgaaagc cagccagatt
2401 cattttccac ggcacatggt ttcctgtggg taagatgtgt ttatttggtt ttggtacaag
2461 ttcacagaga atcaacgttc atggtcggtg tgatgagaga ctgagatgtg aattgctcag
2521 tagagagttg gcggtggcgt atcccagagt gtacttagaa acatgaaaac ttctttgaaa
2581 gggagctgat cacttttgga aaagatttac agttcttagg aatgcaaata tttgtttctt
2641 ttccttttca tctaaaacat ttaccagata aagttgagtc cagagacaac taactaataa
2701 gagaataatt ttaatatgtt tttccatttt ggtgtcaaca ttgcatggat tttttttctt
2761 gttccaagac tggctttttc cagtggaaac ttagcagtta aaattaattt gttccaaatg
2821 aaatattgca tctgaaatta ggctggaatt gcagtagacg ttcctggttc ttgcaaacca
2881 gaggacattc ttgaagctgt gtggtcccag gctctcccct ctctggtttc ctgtgttccc
2941 tccctgcagc ctcagactcg ccctccaggg ctccactgtg cttttccaaa ctctcatcct
3001 ttcctcccag gcgtcctgtg cagcacgcca ctgcctctca cttctcacat ccacagctcc
3061 tgcttggtca gtgttcctag ttgagtgtca gaaattgaac aacccaatta gctggtattt
3121 catttgtacc aacctatgaa tggaggagta gccttaattc ccttgggggc ttccacttct
3181 aagagaactg ttttccgtcc aggtaggcag acctgtcatg gctgaagctt catcacctgg
3241 cctggtcata gcccccaggc cctgtaggca ggacaggggc ttggcagatt ggcatccttc
3301 ccacaaaagc atgaaagtag gatgtctgta tgtagcgaca acaaatacaa gaataaggag
3361 agtttaacaa cattctcaaa gttggtggaa aagaaaaaga atcaatgaaa acatcaccct
3421 aaaccagcta gtgctttctg tttacaggga tcagacccct tcccacttaa aaaaaaaaaa
3481 aaa
//