LOCUS BC036471 1622 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens choline kinase alpha, mRNA (cDNA clone MGC:41867
IMAGE:5265994), complete cds.
ACCESSION BC036471
VERSION BC036471.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1622)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1622)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-AUG-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC036471.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 73 Row: n Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 47078275.
FEATURES Location/Qualifiers
source 1..1622
/db_xref="H-InvDB:HIT000051717"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:41867 IMAGE:5265994"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..1622
/gene="CHKA"
/gene_synonym="CKI"
/db_xref="GeneID:1119"
/db_xref="HGNC:HGNC:1937"
/db_xref="MIM:118491"
CDS 93..1412
/gene="CHKA"
/gene_synonym="CKI"
/codon_start=1
/product="choline kinase alpha"
/protein_id="AAH36471.1"
/db_xref="GeneID:1119"
/db_xref="HGNC:HGNC:1937"
/db_xref="MIM:118491"
/translation="MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLE
SKQLGGQQPPLALPPPPPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGA
WRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQVGAEAMV
LESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELGLPDISAEIAEKMATFH
GMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLE
STPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMY
DYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVN
RFALASHFLWGQWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV"
BASE COUNT 421 a 418 c 429 g 354 t
ORIGIN
1 agtgagagag cggcgagggg gcgcccggcc ggactctgag cctagtcctc tcgcgctgcg
61 gccgcccgcg cctcctcggc cgcctgtcgg gcatgaaaac caaattctgc accgggggcg
121 aggcggagcc ctcgccgctc gggctgctgc tgagctgcgg tagcggcagc gcggccccgg
181 cgcccggcgt ggggcagcag cgcgacgccg ccagcgacct cgagtccaag cagctgggcg
241 gccaacagcc gccgctcgcg ctgccccctc cgccgccgct gccgctgccg ctgccgctgc
301 cccagccccc gccgccgcag ccgcccgcag acgagcagcc ggagccccgg acgcggcgca
361 gggcctatct gtggtgcaag gagttcctgc ccggcgcctg gcggggcctc cgcgaggacg
421 agttccacat cagtgtcatc agaggcggcc ttagcaacat gctgttccag tgctccctac
481 ctgacaccac agccaccctt ggtgatgagc ctcggaaagt gctcctgcgg ctgtatggag
541 cgattttgca ggtgggggct gaggccatgg ttctggagag cgttatgttt gccattctcg
601 cagagaggtc acttgggcca aaactctatg gcatctttcc ccaaggccga ctggagcagt
661 tcatcccgag ccggcgatta gatactgaag aattaggttt gccagatatt tctgcagaaa
721 tcgccgagaa aatggctaca tttcatggta tgaaaatgcc attcaataag gaaccaaaat
781 ggctttttgg cacaatggaa aagtatctaa aggaagtgct gagaattaaa tttactgagg
841 aatccagaat taaaaagctc cacaaattgc tcagttacaa tctgcccttg gaactggaaa
901 acctgagatc attgcttgaa tctactccat ctccagttgt attttgtcat aatgactgtc
961 aagaaggtaa tatcttgttg ctggaaggcc gagagaattc tgaaaaacag aaactgatgc
1021 tcattgattt cgaatacagc agttacaatt acaggggatt cgacattgga aatcacttct
1081 gtgagtggat gtatgattat agctatgaaa aatacccttt tttcagagca aacatccgga
1141 agtatcccac caagaaacaa cagctccatt ttatttccag ttacttgcct gcattccaaa
1201 atgactttga aaacctcagt actgaagaaa aatccattat aaaagaagaa atgttgcttg
1261 aagttaatag gtttgccctt gcatctcatt tcctctgggg acagtggtcc attgtacaag
1321 ccaagatttc atctattgaa tttgggtaca tggactacgc ccaagcaagg tttgatgcct
1381 atttccacca gaagaggaag cttggggtgt gactgtgggg aggactccat ccacctcatc
1441 actggactgc atggggaggc agcagagcgg ggtcccctct gtgcttcgac tactgctcct
1501 gtggcaggag gctttgggtg gctcactact gaacacatgt gtatgatact aaagacggta
1561 ttaaaatgga gcgacgttta aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1621 aa
//