LOCUS BC035813 2080 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens prolyl 4-hydroxylase, alpha polypeptide II, mRNA (cDNA
clone MGC:46171 IMAGE:5753499), complete cds.
ACCESSION BC035813
VERSION BC035813.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2080)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2080)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 79 Row: n Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 63252892.
FEATURES Location/Qualifiers
source 1..2080
/db_xref="H-InvDB:HIT000051520"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:46171 IMAGE:5753499"
/tissue_type="Brain, Lung, Testis, adult, pooled whole"
/clone_lib="NIH_MGC_115"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2080
/gene="P4HA2"
/db_xref="GeneID:8974"
/db_xref="HGNC:HGNC:8547"
/db_xref="MIM:600608"
CDS 62..1663
/gene="P4HA2"
/codon_start=1
/product="prolyl 4-hydroxylase, alpha polypeptide II"
/protein_id="AAH35813.1"
/db_xref="GeneID:8974"
/db_xref="HGNC:HGNC:8547"
/db_xref="MIM:600608"
/translation="MKLWVSALLMAWFGVLSCVQAEFFTSIGHMTDLIYAEKELVQSL
KEYILVEEAKLSKIKSWANKMEALTSKSAADAEGYLAHPVNAYKLVKRLNTDWPALED
LVLQDSAAGFIANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKY
QAMLSVDDCFGMGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATTTKSQVLDYLSYAVF
QLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGI
YERPVDYLPERDVYESLCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEWD
SPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDD
PVVARVNRRMQHITGLTVKTAELLQVANYGVGGQYEPHFDFSRRPFDSGLKTEGNRLA
TFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGC
KWVSNKWFHERGQEFLRPCGSTEVD"
BASE COUNT 524 a 498 c 573 g 485 t
ORIGIN
1 gccgcgggag gttctggaaa cgccgggagc tgcgagtgtc cagacacttc cctctgtgac
61 catgaaactc tgggtgtctg cattgctgat ggcctggttt ggtgtcctga gctgtgtgca
121 ggccgaattc ttcacctcta ttgggcacat gactgacctg atttatgcag agaaagagct
181 ggtgcagtct ctgaaagagt acatccttgt ggaggaagcc aagctttcca agattaagag
241 ctgggccaac aaaatggaag ccttgactag caagtcagct gctgatgctg agggctacct
301 ggctcaccct gtgaatgcct acaaactggt gaagcggcta aacacagact ggcctgcgct
361 ggaggacctt gtcctgcagg actcagctgc aggttttatc gccaacctct ctgtgcagcg
421 gcagttcttc cccactgatg aggacgagat aggagctgcc aaagccctga tgagacttca
481 ggacacatac aggctggacc caggcacaat ttccagaggg gaacttccag gaaccaagta
541 ccaggcaatg ctgagtgtgg atgactgctt tgggatgggc cgctcggcct acaatgaagg
601 ggactattat catacggtgt tgtggatgga gcaggtgcta aagcagcttg atgccgggga
661 ggaggccacc acaaccaagt cacaggtgct ggactacctc agctatgctg tcttccagtt
721 gggtgatctg caccgtgccc tggagctcac ccgccgcctg ctctcccttg acccaagcca
781 cgaacgagct ggagggaatc tgcggtactt tgagcagtta ttggaggaag agagagaaaa
841 aacgttaaca aatcagacag aagctgagct agcaacccca gaaggcatct atgagaggcc
901 tgtggactac ctgcctgaga gggatgttta cgagagcctc tgtcgtgggg agggtgtcaa
961 actgacaccc cgtagacaga agaggctttt ctgtaggtac caccatggca acagggcccc
1021 acagctgctc attgccccct tcaaagagga ggacgagtgg gacagcccgc acatcgtcag
1081 gtactacgat gtcatgtctg atgaggaaat cgagaggatc aaggagatcg caaaacctaa
1141 acttgcacga gccaccgttc gtgatcccaa gacaggagtc ctcactgtcg ccagctaccg
1201 ggtttccaaa agctcctggc tagaggaaga tgatgaccct gttgtggccc gagtaaatcg
1261 tcggatgcag catatcacag ggttaacagt aaagactgca gaattgttac aggttgcaaa
1321 ttatggagtg ggaggacagt atgaaccgca cttcgacttc tctaggcgac cttttgacag
1381 cggcctcaaa acagagggga ataggttagc gacgtttctt aactacatga gtgatgtaga
1441 agctggtggt gccaccgtct tccctgatct gggggctgca atttggccta agaagggtac
1501 agctgtgttc tggtacaacc tcttgcggag cggggaaggt gactaccgaa caagacatgc
1561 tgcctgccct gtgcttgtgg gctgcaagtg ggtctccaat aagtggttcc atgaacgagg
1621 acaggagttc ttgagacctt gtggatcaac agaagttgac tgacatcctt ttctgtcctt
1681 ccccttcctg gtccttcagc ccatgtcaac gtgacagaca cctttgtatg ttcctttgta
1741 tgttcctatc aggctgattt ttggagaaat gaatgtttgt ctggagcaga gggagaccat
1801 actagggcga ctcctgtgtg actgaagtcc cagcccttcc attcagcctg tgccatccct
1861 ggccccaagg ctaggatcaa agtggctgca gcagagttag ctgtctagcg cctagcaagg
1921 tgcctttgta cctcaggtgt tttaggtgtg agatgtttca gtgaaccaaa gttctgatac
1981 cttgtttaca tgtttgtttt tatggcattt ctatctattg tggctttacc aaaaaataaa
2041 atgtccctac cagaaaaaaa aaaaaaaaaa aaaaaaaaaa
//