LOCUS BC035633 1787 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens G protein-coupled receptor 65, mRNA (cDNA clone
MGC:45304 IMAGE:5218256), complete cds.
ACCESSION BC035633
VERSION BC035633.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1787)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1787)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 68 Row: p Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33695103.
FEATURES Location/Qualifiers
source 1..1787
/db_xref="H-InvDB:HIT000051451"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45304 IMAGE:5218256"
/tissue_type="Blood, adult leukocytes"
/clone_lib="NIH_MGC_118"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1787
/gene="GPR65"
/gene_synonym="hTDAG8"
/gene_synonym="TDAG8"
/db_xref="GeneID:8477"
/db_xref="HGNC:HGNC:4517"
/db_xref="MIM:604620"
CDS 531..1544
/gene="GPR65"
/gene_synonym="hTDAG8"
/gene_synonym="TDAG8"
/codon_start=1
/product="G protein-coupled receptor 65"
/protein_id="AAH35633.1"
/db_xref="GeneID:8477"
/db_xref="HGNC:HGNC:4517"
/db_xref="MIM:604620"
/translation="MNSTCIEEQHDLDHYLFPIVYIFVIIVSIPANIGSLCVSFLQAK
KESELGIYLFSLSLSDLLYALTLPLWIDYTWNKDNWTFSPALCKGSAFLMYMNFYSST
AFLTCIAVDRYLAVVYPLKFFFLRTRRFALMVSLSIWILETIFNAVMLWEDETVVEYC
DAEKSNFTLCYDKYPLEKWQINLNLFRTCTGYAIPLVTILICNRKVYQAVRHNKATEN
KEKKRIIKLLVSITVTFVLCFTPFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITV
ALTSLNCVADPILYCFVTETGRYDMWNILKFCTGRCNTSQRQRKRILSVSTKDTMELE
VLE"
BASE COUNT 598 a 327 c 325 g 537 t
ORIGIN
1 ttcttgactt gatgcaggca cagatttatc aagctcctca gtcaacaaac acatcaccgg
61 aagaaacatg gaaggaaagg aattttaaaa ggaaatacca atctctgtgc aaacaaagcc
121 ttgtatattc atgtttgcac caatctactg tgagatttat gaagaaaaac aaattgcgga
181 caactctcta tgtacactta caaatgcctc agttgatgct tgtgggctgt ttgtcagcgt
241 tctgtgataa tgaacacatg gacttctgtt tattaaattc agttgacccc tttagccaat
301 tgccaggagc ctggattttt acttccaact gctgatatct gtgtaaaaat tgatctacat
361 ccacccttta aaagcattga tgaattaatt agaactttag acaacaaaga aaaattgaaa
421 aagaattctc agtaaaagcg aattcgatgt tcaaaacaaa ctacaaagag acaagacttc
481 tctgtttact ttctaagaac taatataatt gctaccttaa aaaggaaaaa atgaacagca
541 catgtattga agaacagcat gacctggatc actatttgtt tcccattgtt tacatctttg
601 tgattatagt cagcattcca gccaatattg gatctctgtg tgtgtctttc ctgcaagcaa
661 agaaggaaag tgaactagga atttacctct tcagtttgtc actatcagat ttactctatg
721 cattaactct ccctttatgg attgattata cttggaataa agacaactgg actttctctc
781 ctgccttgtg caaagggagt gcttttctca tgtacatgaa tttttacagc agcacagcat
841 tcctcacctg cattgccgtt gatcggtatt tggctgttgt ctaccctttg aagttttttt
901 tcctaaggac aagaagattt gcactcatgg tcagcctgtc catctggata ttggaaacca
961 tcttcaatgc tgtcatgttg tgggaagatg aaacagttgt tgaatattgc gatgccgaaa
1021 agtctaattt tactttatgc tatgacaaat accctttaga gaaatggcaa atcaacctca
1081 acttgttcag gacgtgtaca ggctatgcaa tacctttggt caccatcctg atctgcaacc
1141 ggaaagtcta ccaagctgtg cggcacaata aagccacgga aaacaaggaa aagaagagaa
1201 tcataaaact acttgtcagc atcacagtta cttttgtctt atgctttact ccctttcatg
1261 tgatgttgct gattcgctgc attttagagc atgctgtgaa cttcgaagac cacagcaatt
1321 ctgggaagcg aacttacaca atgtatagaa tcacggttgc attaacaagt ttaaattgtg
1381 ttgctgatcc aattctgtac tgttttgtaa ccgaaacagg aagatatgat atgtggaata
1441 tattaaaatt ctgcactggg aggtgtaata catcacaaag acaaagaaaa cgcatacttt
1501 ctgtgtctac aaaagatact atggaattag aggtccttga gtagaaccaa ggatgttttg
1561 aagggaaggg aagtttaagt tatgcattat tatatcatca agattacatt ttgaaaagga
1621 aatctagcat gtgaggggac taagtgttct cagagtgatg ttttaatcca gtccaataaa
1681 aatatcttaa aactgcattg tacagctccc tccctgcgtt ttattaaatg atgtatatta
1741 aacaaagatc aataaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
//