LOCUS BC035487 3057 bp mRNA linear HTC 27-OCT-2008
DEFINITION Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone
IMAGE:4779421), with apparent retained intron.
ACCESSION BC035487
VERSION BC035487.1
KEYWORDS HTC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3057)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3057)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: James Cleaver, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 52 Row: d Column: 7
This clone has the following problem: retained intron.
FEATURES Location/Qualifiers
source 1..3057
/db_xref="H-InvDB:HIT000093940"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4779421"
/tissue_type="Skin, normal"
/clone_lib="NCI_CGAP_Skn3"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6.1"
BASE COUNT 671 a 883 c 721 g 782 t
ORIGIN
1 atctaggtct actgcctcca aagccagtct tctctcctgc aacatcatgc tctgtctagc
61 aggagatgag aacaggtctc catttggagc ctgtcagtgg ggtcagagac taagattcag
121 gctcagggtc taaattccat atcctttctt ccataccctg gtgtttccta tgaacagata
181 gatactttag ggctgcaagg tttggattgc atggcactgc tcagaagata agttacaggt
241 ctgggctagg ctgtagctgc ccctccaggt ggctagacct ttcctttctg tgtcaccagt
301 taacactggc caacagttcc ttccattaac tgttcactgc tttctcctgt gtctaactga
361 tgcagtttat gacccataac taagagcagt accaggtatg gctctgtttc ctgttcatgt
421 cccctgtcct ctgggctgca tgcattccgt tcttacagaa agaatacctt taacctagta
481 catcctgcca cacatctgct tctactgtga aattgatgag ggggtattac cgattcttcc
541 ctcacccatc atttactgag atgctggtga ttgcattata atcctctaaa gcttacattg
601 tctttctgat tcttggtctt atctgagcaa gtgatctata aataactcag tggctttctc
661 atgactgttt taattattag attttaatca agtgtcttat taaatatatc tgcatgcttc
721 cacaggcatc tgtctcttca catggctgtt cagtgtgcct ctcacaagtt agcccacgtt
781 ttctgttctc ctgcttcaaa ctcagttgag ctgccttgct ttggctttga tcccagcttt
841 ccagcgctgc tcaatctgtt gccatggcag gccattggaa aggctcagtg catccccgtg
901 cctgaagcca agtgagcgct cactccatgc atgcatggag gctgggcagg agcctgccta
961 atcaaccagc catgtgagga gggagggcct gttccttcct gtaagctatg tcatgaggca
1021 gcgtggtcaa gtcctctgcc agggagtggc ctgggcccag cctgggcatg ttttcatgcc
1081 agggtgctag agcctactgc cagattgtct ccctccaccc ccaatgaaaa aatccttcca
1141 gaagggaaga gccaatttcc cctgtattgg aggggaagtg gcagcacctc ctgaagcagt
1201 tggactttca tcaccctacc tctgcatctg cctgaaggac agatttagcc aattaaccta
1261 aggttacctt cctctctgat aaattcccca ttctgtcttc ccatgtgttg tgtctcgttt
1321 ttttcctcct ccttccctct tccttgcccc ctcttcccct aaaccttaca gatagctcgt
1381 gaggctgagg cagccatgtt ccaccgcaag ctgtttgaag aacttgtgcg agcctcaagt
1441 cactccacag acctcatgga agccatggcc atgggcagcg tggaggcttc ttataagtgt
1501 ttagcagcag ctttgatagt tctgacggag tctggcaggt agggccctaa gggcaggtaa
1561 cactgttagg ataaccagcc tcttgctcca cctgctctag gagaagacag ccaggcccaa
1621 cctggcatct gggcacagag cctcttctcg tctgtaggaa caccgccagg gaggtcatgg
1681 cagggcagga ccaaagggtc ctgtggctca gtaggcacag tagatgtcac aggcacttgg
1741 tgaaggactg gtttctgtgg agtcttgatc ttggctcagc tcagaatctc cagtgattgg
1801 gctcctcttg gcctttgttc ccaggaacat gttcctcacc agctgtccgg tgactcttcc
1861 cctccctctc cttttgtgac aaagctctgt ccccctctcg tccctctgga cggatgttgc
1921 tcccctagat tgcccgtgag gcagaggctg ccatctacca cttgcaatta tttgaggaac
1981 tccgccgcct ggcgcccatt accagcgacc ccacagaagc caccgccgtg ggtgccgtgg
2041 aggcctcctt caagtgctgc agtggggcca taatcgtcct caccaagtct ggcaggtctg
2101 ctcaccaggt ggccagatac cgcccacgtg cccccatcat tgctgtgacc cggaatcccc
2161 agacagctcg tcaggcccac ctgtaccgtg gcatcttccc tgtgctgtgc aaggacccag
2221 tccaggaggc ctgggctgag gacgtggacc tccgggtgaa ctttgccatg aatgttggca
2281 aggcccgagg cttcttcaag aagggagatg tggtcattgt gctgaccgga tggcgccctg
2341 gctccggctt caccaacacc atgcgtgttg ttcctgtgcc gtgatggacc ccagagcccc
2401 tcctccagcc cctgtcccac ccccttcccc cagcccatcc attaggccag caacgcttgt
2461 agaactcact ctgggctgta acgtggcact ggtaggttgg gacaccaggg aagaagatca
2521 acgcctcact gaaacatggc tgtgtttgca gcctgctcta gtgggacagc ccagagcctg
2581 gctgcccatc atgtggcccc acccaatcaa gggaagaagg aggaatgctg gactggaggc
2641 ccctggagcc agatggcaag agggtgacag cttcctttcc tgtgtgtact ctgtccagtt
2701 cctttagaaa aaatggatgc ccagaggact cccaaccctg gcttggggtc aagaaacagc
2761 cagcaagagt taggggcctt agggcactgg gctgttgttc cattgaagcc gactctggcc
2821 ctggccctta cttgcttctc tagctctcta ggcctctcca gtttgcacct gtccccaccc
2881 tccactcagc tgtcctgcag caaacactcc accctccacc ttccattttc ccccactact
2941 gcagcacctc caggcctgtt gctatagagc ctacctgtat gtcaataaac aacagctgaa
3001 gcaccaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
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