LOCUS BC035198 2286 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:9369
IMAGE:3859987), complete cds.
ACCESSION BC035198
VERSION BC035198.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2286)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2286)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 21 Row: d Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..2286
/db_xref="H-InvDB:HIT000051345"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9369 IMAGE:3859987"
/tissue_type="Ovary, adenocarcinoma"
/clone_lib="NIH_MGC_66"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2286
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="MGC3932"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/db_xref="GeneID:5315"
/db_xref="HGNC:HGNC:9021"
/db_xref="MIM:179050"
CDS 55..1650
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="MGC3932"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/codon_start=1
/product="pyruvate kinase, muscle"
/protein_id="AAH35198.1"
/db_xref="GeneID:5315"
/db_xref="HGNC:HGNC:9021"
/db_xref="MIM:179050"
/translation="MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARN
TGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDP
ILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLD
YKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLVSKKGVNLPGAAVDL
PAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEG
VRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESM
IKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHL
QLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPI
IAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDV
VIVLTGWRPGSGFTNTMRVVPVP"
BASE COUNT 523 a 639 c 641 g 483 t
ORIGIN
1 cttcgtcttt gcagcgtagc ccgagtcggt cagcgccgga ggacctcagc agccatgtcg
61 aagccccata gtgaagccgg gactgccttc attcagaccc agcagctgca cgcagccatg
121 gctgacacat tcctggagca catgtgccgc ctggacattg attcaccacc catcacagcc
181 cggaacactg gcatcatctg taccattggc ccagcttccc gatcagtgga gacgttgaag
241 gagatgatta agtctggaat gaatgtggct cgtctgaact tctctcatgg aactcatgag
301 taccatgcgg agaccatcaa gaatgtgcgc acagccacgg aaagctttgc ttctgacccc
361 atcctctacc ggcccgttgc tgtggctcta gacactaaag gacctgagat ccgaactggg
421 ctcatcaagg gcagcggcac tgcagaggtg gagctgaaga agggagccac tctcaaaatc
481 acgctggata acgcctacat ggaaaagtgt gacgagaaca tcctgtggct ggactacaag
541 aacatctgca aggtggtgga agtgggcagc aagatctacg tggatgatgg gcttatttct
601 ctccaggtga agcagaaagg tgccgacttc ctggtgacgg aggtggaaaa tggtggctcc
661 ttggtcagca agaagggtgt gaaccttcct ggggctgctg tggacttgcc tgctgtgtcg
721 gagaaggaca tccaggatct gaagtttggg gtcgagcagg atgttgatat ggtgtttgcg
781 tcattcatcc gcaaggcatc tgatgtccat gaagttagga aggtcctggg agagaaggga
841 aagaacatca agattatcag caaaatcgag aatcatgagg gggttcggag gtttgatgaa
901 atcctggagg ccagtgatgg gatcatggtg gctcgtggtg atctaggcat tgagattcct
961 gcagagaagg tcttccttgc tcagaagatg atgattggac ggtgcaaccg agctgggaag
1021 cctgtcatct gtgctactca gatgctggag agcatgatca agaagccccg ccccactcgg
1081 gctgaaggca gtgatgtggc caatgcagtc ctggatggag ccgactgcat catgctgtct
1141 ggagaaacag ccaaagggga ctatcctctg gaggctgtgc gcatgcagca cctgattgcc
1201 cgtgaggcag aggctgccat ctaccacttg caattatttg aggaactccg ccgcctggcg
1261 cccattacca gcgaccccac agaagccacc gccgtgggtg ccgtggaggc ctccttcaag
1321 tgctgcagtg gggccataat cgtcctcacc aagtctggca ggtctgctca ccaggtggcc
1381 agataccgcc cacgtgcccc catcattgct gtgacccgga atccccagac agctcgtcag
1441 gcccacctgt accgtggcat cttccctgtg ctgtgcaagg acccagtcca ggaggcctgg
1501 gctgaggacg tggacctccg ggtgaacttt gccatgaatg ttggcaaggc ccgaggcttc
1561 ttcaagaagg gagatgtggt cattgtgctg accggatggc gccctggctc cggcttcacc
1621 aacaccatgc gtgttgttcc tgtgccgtga tggaccccag agcccctcct ccagcccctg
1681 tcccaccccc ttcccccagc ccatccatta ggccagcaac gcttgtagaa ctcactctgg
1741 gctgtaacgt ggcactggta ggttgggaca ccagggaaga agatcaacgc ctcactgaaa
1801 catggctgtg tttgcagcct gctctagtgg gacagcccag agcctggctg cccatcatgt
1861 ggccccaccc aatcaaggga agaaggagga atgctggact ggaggcccct ggagccagat
1921 ggcaagaggg tgacagcttc ctttcctgtg tgtactctgt ccagttcctt tagaaaaaat
1981 ggatgcccag aggactccca accctggctt ggggtcaaga aacagccagc aagagttagg
2041 ggccttaggg cactgggctg ttgttccatt gaagccgact ctggccctgg cccttacttg
2101 cttctctagc tctctaggcc tctccagttt gcacctgtcc ccaccctcca ctcagctgtc
2161 ctgcagcaaa cactccaccc tccaccttcc attttccccc actactgcag cacctccagg
2221 cctgttgcta tagagcctac ctgtatgtca ataaacaaca gctgaagcac caaaaaaaaa
2281 aaaaaa
//