LOCUS BC034500 3889 bp mRNA linear HUM 03-NOV-2008
DEFINITION Homo sapiens LAG1 homolog, ceramide synthase 3, mRNA (cDNA clone
MGC:26926 IMAGE:4838423), complete cds.
ACCESSION BC034500
VERSION BC034500.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3889)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3889)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (11-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 34 Row: o Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31341477.
FEATURES Location/Qualifiers
source 1..3889
/db_xref="H-InvDB:HIT000051172"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:26926 IMAGE:4838423"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3889
/gene="LASS3"
/gene_synonym="CerS3"
/db_xref="GeneID:204219"
/db_xref="HGNC:HGNC:23752"
CDS 384..1532
/gene="LASS3"
/gene_synonym="CerS3"
/codon_start=1
/product="LASS3 protein"
/protein_id="AAH34500.1"
/db_xref="GeneID:204219"
/db_xref="HGNC:HGNC:23752"
/translation="MFWTFKEWFWLERFWLPPTIKWSDLEDHDGLVFVKPSHLYVTIP
YAFLLLIIRRVFEKFVASPLAKSFGIKETVRKVTPNTVLENFFKHSTRQPLQTDIYGL
AKKCNLTERQVERWFRSRRNQERPSRLKKFQEACWRFAFYLITVAGIAFLYDKPWLYD
LWEVWNGYPKQPLLPSQYWYYILEMSFYWSLLFRLGFDVKRKDFLAHIIHHLAAISLM
SFSWCANYIRSGTLVMIVHDVADIWLESAKMFSYAGWTQTCNTLFFIFSTIFFISRLI
VFPFWILYCTLILPMYHLEPFFSYIFLNLQLMILQVLHLYWGYYILKMLNRCIFMKSI
QDVRSDDEDYEEEEEEEEEEATKGKEMDCLKNGLGAERHLIPNGQHGH"
BASE COUNT 1072 a 860 c 859 g 1098 t
ORIGIN
1 agcggggtcc aggacggagg aggaggcagg tggtgaaaca ggaagcaaca tcccatcagg
61 aagaacctga gggtgccatc cagtagcttc gcctcacgtc actgactgcc ctcagtcaga
121 aatcttgact cgcaactttg gagggtgggt ctctgaagga atttcaggtc ttacacagag
181 atgagttgtg ctgctctctg aggagaacga agctggttgg aacgttggaa gctgctctct
241 gactacactt cacaagcaag gggcaccttt tgtggactga catttcagaa agggatgttg
301 tgaaacaaaa gctgacattt atatatatat acatatatac agtatttgag ttcctcagta
361 gaaagctatc atatatactc agaatgtttt ggacgtttaa agaatggttc tggttggaaa
421 gattctggct tcctccaaca ataaagtggt cagatcttga ggatcacgat ggactcgtct
481 ttgtaaaacc ttctcattta tacgtgacaa ttccatatgc ttttctcttg ctgattatca
541 ggcgtgtatt tgaaaaattt gttgcttcac ctctagcaaa atcatttggc attaaagaga
601 cagttcgaaa ggttacacca aatactgtct tagagaattt tttcaaacat tccacaaggc
661 aaccattgca aactgatatt tatggactgg caaagaagtg taacttgacg gagcgccagg
721 tggaaagatg gtttaggagt cggcggaatc aagagaggcc ttccaggctg aagaaattcc
781 aggaagcttg ctggagattt gcattttact tgatcactgt tgctggaatt gcgtttcttt
841 atgataaacc ttggctatat gacttatggg aggtttggaa tggctatccc aaacagcccc
901 tgctgccatc ccagtactgg tactacattt tagaaatgag tttttattgg tctctgttat
961 ttagacttgg ctttgatgtc aagagaaagg attttctagc tcatatcatc caccacctgg
1021 ctgctattag tctgatgagc ttctcttggt gtgctaatta tattcgcagt gggaccctcg
1081 tgatgattgt acacgatgtg gctgacattt ggctggagtc tgctaagatg ttttcttatg
1141 ctggatggac gcagacctgt aacaccctgt ttttcatctt ctccaccata tttttcatca
1201 gccgcctcat tgtttttcct ttctggattt tatattgcac gctgatcttg cctatgtatc
1261 acctcgagcc tttcttttca tacatcttcc tcaacctaca gctcatgatc ttgcaggtcc
1321 ttcaccttta ctggggttat tacatcttga agatgctcaa cagatgtata ttcatgaaga
1381 gcatccagga tgtgaggagt gatgacgagg attatgaaga ggaagaggaa gaggaagaag
1441 aagaggctac caaaggcaaa gagatggatt gtttaaagaa cggcctcggg gctgagaggc
1501 acctcattcc caatggccag catggccatt agctggaagc ctacaggact cccatggcac
1561 agcatgctgc aagtactgtt ggcagcctgg cttccaggcc ccacacagac cccacattct
1621 gcccttccct ctttctcacc accgccttcc ctcccaccta agatgtgttt accaaaatgt
1681 tgttaacttg tgttaaaatg ttaaatataa gcatgcccat ggatttttac tgcagttagg
1741 actcagactg gtcaaagatt tcaaagattt ctccacagaa ccgtctcagt tctaattgca
1801 ctccctcatg catgtcactt tctcaggggc tcgctttgtt atagaccctt tcgcctcgcc
1861 accttgcctg tcctcaggac gctttcacag gtgctaagtg atctcatttt tcccaggtgt
1921 gtttggccac aaagagcagc ttctttctca aaatgagtta gaagtggcag tgggacagga
1981 gcggaaggac cacaccagga gacacttcca tcctgaagct taggtgcctc atctccacag
2041 ggcggtggca gtccctgcct gccaccccac agggttgcag caaggattaa gtgagatcac
2101 agaagtttaa gtacacactg tcaaccgtgg ggtcatcgtg aaccaaccca ctttgcactg
2161 tttgggagac agaaacctgg ctaaacatgg cactgcaatg ggcctgaaca aaaggcagaa
2221 tctttaaaaa ttcctcttaa atgactctgg agatacctgg aaatgaaagt gccaagaaag
2281 gtgttccatt ttatttgcta actatttatg cattagctcc ccaagggtca ttctcagatt
2341 ctcctggaat tcttctccct caggacccat ggttcaagga aggaagctta ggccctgttc
2401 cttcctgtcc tttgctggtc tttccctttt ttcctttcta ggagggaagc ttctgtgctg
2461 ctgccctgag cccttccttc aggccagcac agtacctggg gacctccacg ggggaatggg
2521 atccaggcca ggttgcttgc tgagcctcat cacccaggag gcctgagcct ctggggaggg
2581 cacgcatgta cactgccaga cccaggggag atcttgggaa cagaggatgc tacgtgattt
2641 cctctggctt ccaacccaat cagcctgcat cacagtgaaa cacaacacaa aaggccataa
2701 aaggcatacc cctgagaaat gtttaagggc aggtgctaga gtcagtgcca gctccctggg
2761 aggagggata gggacgggca gatcagtagc cagcttgtcc tcaccctcag gaagggagca
2821 ccggagaaga cttgcacacg tccctgcctc ccttgcggcc ttgtcaccag agggacacat
2881 ctcctgggca caatgtgcag ggctgacctg ggagacttct caggtggctg ctggctgagg
2941 agaggccagg ccttcccaag gaagaccctg agtaaaatct gcatctgtcc tcaccacctg
3001 gcacagtctc tcataacggt cagattttgt atgttcatcc tatttattca ggggctcgta
3061 ctagaaagcc acaggagagg tcggcttgta gggactggaa aatcagcccc aagcagagca
3121 gctgcagggc ccctggagcc ggaaggacac tgcacagaac agactgcgtt attgttattt
3181 ttaaataaaa atatacattt gaaaccttaa ggctaaacaa aaataaacaa aaaatccttt
3241 agaattcttt ccacaacaat atctctttct gagaaattgt tacaaacaag gtcagatttt
3301 ctctgtataa catttgcttt tatgaggaca atatcatatg cattatatgc ataatatgat
3361 attataaatc aaaatgcctg cacccacttt agggtatagc tattgactta ttattaatat
3421 tatattatta ttattttgct ggaagaaggt cacactaaga tataattttt tatgttttca
3481 gttaacggta tgttttcttc tttgcttatt tggtttttgt ctctgtacca aatatcttct
3541 tgcttaaggt agaaaagtat ttgtttacct ctatctccag ttttttttct tatttgaatg
3601 ttgaaggtaa aattgatata ccaattttaa ctatttctga tacagctgaa agcactaaac
3661 tacttcataa gaagtagata ctcatttttg taacactatt tagggctttt gtggttaatt
3721 ttaaaggaaa ccactctttc tacaggaaac aagggctcag gattcttcag atgaccttat
3781 aaaaatgcag tccacagtgc tatcaatatt gtaacagtaa tgactacaat aaagccaaaa
3841 gtccagtgta aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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