LOCUS BC034144 1284 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens polymerase (RNA) II (DNA directed) polypeptide E,
25kDa, mRNA (cDNA clone MGC:32760 IMAGE:4617000), complete cds.
ACCESSION BC034144
VERSION BC034144.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1284)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1284)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 41 Row: g Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1284
/db_xref="H-InvDB:HIT000259073"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:32760 IMAGE:4617000"
/tissue_type="Lung"
/clone_lib="NIH_MGC_77"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1284
/gene="POLR2E"
/gene_synonym="hRPB25"
/gene_synonym="hsRPB5"
/gene_synonym="RPABC1"
/gene_synonym="RPB5"
/gene_synonym="XAP4"
/db_xref="GeneID:5434"
/db_xref="HGNC:HGNC:9192"
/db_xref="MIM:180664"
CDS 43..675
/gene="POLR2E"
/gene_synonym="hRPB25"
/gene_synonym="hsRPB5"
/gene_synonym="RPABC1"
/gene_synonym="RPB5"
/gene_synonym="XAP4"
/codon_start=1
/product="polymerase (RNA) II (DNA directed) polypeptide
E, 25kDa"
/protein_id="AAH34144.1"
/db_xref="GeneID:5434"
/db_xref="HGNC:HGNC:9192"
/db_xref="MIM:180664"
/translation="MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQF
GDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRA
LIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELL
ARYKLRENQLPRIQAGDPVARYFGVKRGQVVKIIRPSETAGRYITYRLVQ"
BASE COUNT 276 a 399 c 389 g 220 t
ORIGIN
1 cgcggagaag gggtgcgagc ggcggcggcg gcggaggctg ccatggacga cgaggaggag
61 acgtaccggc tctggaaaat ccgcaagacc atcatgcagc tgtgccacga ccgtggctat
121 ctggtgaccc aggacgagct tgaccagacc ctggaggagt tcaaagccca atttggggac
181 aagccgagtg aggggcggcc gcggcgcacg gacctcaccg tgctggtggc ccacaacgat
241 gaccccaccg accagatgtt tgtgttcttt ccagaggagc ccaaggtggg catcaagacc
301 atcaaggtgt actgccagcg catgcaggag gagaacatca cacgggctct catcgtggtg
361 cagcagggca tgacaccctc cgccaagcag tccctggtcg acatggcccc caagtacatc
421 ctggagcagt ttctgcagca ggagctgctc atcaacatca cggagcacga gctagtccct
481 gagcacgtcg tcatgaccaa ggaggaggtg acagagctgc tggcccgata taagctccga
541 gagaaccagc tgcccaggat ccaggcgggg gaccctgtgg cgcgctactt tggggtaaag
601 cgtgggcagg tggtgaagat catccggccc agtgagacgg ctggcaggta catcacctac
661 cggctggtgc agtagctacc gcctgacagc ccctagaggc ggacacacag cgacccccat
721 ccctgcagga caaacgcccc tgccctgcca gaatccggcc cccacagctg tcacggctgc
781 tgctcctctg gactccccaa ggcaggtggc ctccacccac gttctcccgt cctggggtga
841 ggcttcctgt ggcccagccc gccccattca cctgtggatt tgtgcgagat gcagcctcag
901 aaggaacaag gcccccagag ggaggtcacc tgggggcagc tggtgccggg tcttcaccca
961 gaccacgctg ggtcccctct gttgggggtt tggggtccgg gtctcccacc agccactgct
1021 tcctccaggg ccctcggcct tccacccctc gtcttccctc cctcgggggc cctgatgcgt
1081 ggcggccccc gcccggcctc ggctctttac tccattcaca gccgtgcacg cgctcaagcc
1141 accagggtgc gagatgccag ctctggagtt ctcggttgtt gtaggaggtt gggtgttttc
1201 aaatggtaaa gatgttttga gcaaataaat ttgcttgata cagaaaccag gccgaaaaaa
1261 aaaaaaaaaa aaaaaaaaaa aaaa
//