LOCUS BC033708 1678 bp mRNA linear HUM 01-JUN-2004
DEFINITION Homo sapiens Ral GEF with PH domain and SH3 binding motif 1, mRNA
(cDNA clone MGC:45125 IMAGE:3901593), complete cds.
ACCESSION BC033708
VERSION BC033708.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1678)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1678)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 68 Row: c Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7662069.
FEATURES Location/Qualifiers
source 1..1678
/db_xref="H-InvDB:HIT000041911"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45125 IMAGE:3901593"
/tissue_type="Pancreas, epithelioid carcinoma"
/clone_lib="NIH_MGC_70"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1678
/gene="RALGPS1"
/gene_synonym="KIAA0351"
/gene_synonym="RALGEF2"
/gene_synonym="RALGPS1A"
/db_xref="GeneID:9649"
CDS 298..1452
/gene="RALGPS1"
/gene_synonym="KIAA0351"
/gene_synonym="RALGEF2"
/gene_synonym="RALGPS1A"
/codon_start=1
/product="RALGPS1 protein"
/protein_id="AAH33708.1"
/db_xref="GeneID:9649"
/translation="MYKRNGLMASVLVTSATPQGSSSSDSLEGQSCDYASKSYDAVVF
DVLKVTPEEFASQITLMDIPVFKAIQPEELASCGWSKKEKHSLAPNVVAFTRRFNQVS
FWVVREILTAQTLKIRAEILSHFVKIAKKLLELNNLHSLMSVVSALQSAPIFRLTKTW
ALLNRKDKTTFEKLDYLMSKEDNYKRTREYIRSLKMVPSIPYLGIYLLDLIYIDSAYP
ASGSIMENEQRSNQMNNILRIIADLQVSCSYDHLTTLPHVQKYLKSVRYIEELQKFVE
DDNYKLSLRIEPGSSSPRLVSSKEDLAVSHLSSLSHQGQAEEARLKPTSGQHPAWMWP
SSSRVPAAPPASAAPRSPWPRNLRNDQGQPGAVALTCNPSTLGSRSRRIT"
BASE COUNT 432 a 464 c 430 g 352 t
ORIGIN
1 ccacgcgtcc ggcggacggt tcctactgcg gctgggcacc ggctccgctc ccgcgtctgc
61 ccgcgctcca gctgcgcctg gcccggcccc ggcccggctc ggcgtggccc cggcctccaa
121 gcgaaggcgc cgctgccgct gggccgctcc cagggccatg aggaagcggc ggcagccact
181 gcggcccgcg tcaaggtgac cggccgggac tgcggcggcg gggagagcgg cggacttctc
241 cagacaggtt atgttacctg cagaggctgc cctgaagctc cctgtggcct ggagactatg
301 tacaagagga atggtctgat ggctagcgtg ttggtcacct ctgccactcc acagggcagc
361 agcagctcgg actctctgga gggccagagc tgcgactatg ccagcaagag ctatgatgcc
421 gttgtcttcg atgttttgaa agtgacccca gaggagtttg ctagccagat tacattaatg
481 gatatacctg tgtttaaagc tatccagccg gaggaactag ccagctgtgg atggagtaag
541 aaggagaaac acagtcttgc ccctaacgtt gtggccttta cccggaggtt taaccaggtc
601 agtttttggg ttgtacgaga aattctaaca gcacagactt taaaaataag ggcagaaatc
661 ctcagccatt ttgtgaaaat agccaagaaa cttctagaac tcaacaacct tcattctctc
721 atgtctgtgg tatcagcatt acaaagtgct cccatcttca ggctgacaaa aacctgggct
781 cttttaaatc gaaaagacaa gactaccttt gagaaattgg actacctgat gtcgaaagaa
841 gataattaca agcggacacg ggaatatatc cgaagcttga agatggttcc aagtattccc
901 tatctaggaa tctatcttct ggatttaatc tacattgatt ctgcatatcc tgcctcaggc
961 agtatcatgg aaaatgaaca aagatccaat cagatgaaca atattcttcg aataattgct
1021 gatttacaag tttcctgcag ctatgatcac ctcaccaccc tgccccatgt gcagaagtac
1081 ctgaagtccg tacgctacat tgaagagctc cagaagtttg tggaagacga caactacaaa
1141 ctgtcgctca gaatcgaacc aggaagcagc tctccaagac tagtctcttc caaggaagat
1201 cttgcagtct cccatctcag cagcctgtca catcaaggtc aagcagaaga ggcaagactc
1261 aaacccacct ctggccaaca ccctgcctgg atgtggccca gctcctcacg agtaccagcg
1321 gctcccccag catctgctgc tccgcgctcc ccgtggccta ggaatctaag aaatgatcaa
1381 ggtcaaccag gtgcagtggc tctcacctgt aatcccagca ctttgggaag ccgaagcagg
1441 aggatcactt gagcccagga gttcaagacc agcctgggca acatggcaaa accccatctc
1501 tacaaaagta tataaatgat ctgggcatgg tggtgcgtgc ctgcagcccc agctcctcag
1561 ggcttaggcg agatcgcttg atcccaggag ggggaggcta cagtgagccg tgatcgcgcc
1621 accgcgctcc agcttgggtg acagagcaag accttgtctc aagaaaaaaa aaaaaaaa
//