LOCUS BC033307 4174 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens mannosidase, alpha, class 2B, member 2, mRNA (cDNA
clone MGC:24942 IMAGE:4932398), complete cds.
ACCESSION BC033307
VERSION BC033307.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4174)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4174)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 29 Row: n Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 50659092.
FEATURES Location/Qualifiers
source 1..4174
/db_xref="H-InvDB:HIT000093033"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:24942 IMAGE:4932398"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4174
/gene="MAN2B2"
/gene_synonym="KIAA0935"
/db_xref="GeneID:23324"
/db_xref="HGNC:HGNC:29623"
CDS 7..3036
/gene="MAN2B2"
/gene_synonym="KIAA0935"
/codon_start=1
/product="mannosidase, alpha, class 2B, member 2"
/protein_id="AAH33307.1"
/db_xref="GeneID:23324"
/db_xref="HGNC:HGNC:29623"
/translation="MGQLCWLPLLAPLLLLRPPGVQSAGPIRAFVVPHSHMDVGWVYT
VQESMRAYAANVYTSVVEELARGQQRRFIAVEQEFFRLWWDGVASDQQKYQVRQLLEE
GRLEFVIGGQVMHDEAVTHLDDQILQLTEGHGFLYETFGIRPQFSWHVDPFGASATTP
TLFALAGFNAHLGSRIDYDLKAAMQEARGLQFVWRGSPSLSERQEIFTHIMDQYSYCT
PSHIPFSNRSGFYWNGVAVFPKPPPDGVYPNMSEPVTPANINLYAEALVANVKQRAAW
FRTPHVLWPWGCDKQFFNASVQFANMDPLLDHINSHAAELGVSVQYATLGDYFRALHA
LNVTWRVRDHHDFLPYSTEPFQAWTGFYTSRSSLKGLARRASALLYAGESMFTRYLWP
APRGHLDPTWALQQLQQLRWAVSEVQHHDAITGTESPKVRDMYATHLASGMLGVRKLM
ASIVLDELQPQAPMAASSDAGPAGHFASVYNPLAWTVTTIVTLTVGFPGVRVTDEAGH
PVPSQIQNSTETPSAYDLLILTTIPGLSYRHYSIRPTAGAQEGTQEPAATVASTLQFG
RRLRRRTSHAGRYLVPVANDCYIVLLDQDTNLMHSIWERQSNRTVRVTQEFLEYHVNG
DVKQGPISDNYLFTPGKAAVPAWEAVEMEIVAGQLVTEIRQYFYRNMTAQNYTYAIRS
RLTHVPQGHDGELLCHRIEQEYQAGPLELNREAVLRTSTNLNSQQVIYSDNNGYQMQR
RPYVSYVNNSIARNYYPMVQSAFMEDGKSRLVLLSERAHGISSQGNGQVEVMLHRRLW
NNFDWDLGYNLTLNDTSVVHPVLWLLLGSWSLTTALRQRSALALQHRPVVLFGDLAGT
APKLPGPQQQEAVTLPPNLHLQILSIPGWRYSSNHTEHSQNLRKGHRGEAQADLRRVL
LRLYHLYEVGEDPVLSQPVTVNLEAVLQALGSVVAVEERSLTGTWDLSMLHRWSWRTG
PGRHRGDTTSPSRPPGGPIITVHPKEIRTFFIHFQQQ"
BASE COUNT 961 a 1270 c 1155 g 788 t
ORIGIN
1 gcagggatgg ggcagctgtg ctggctgccg ctgctggcac cgctcctgtt gctgcgaccg
61 ccaggggtcc agtccgccgg ccccatccgg gccttcgtgg tgccccacag ccacatggac
121 gtgggctggg tctacactgt gcaggaaagc atgcgggcgt acgccgccaa tgtctacacc
181 tcagtggtgg aagagctggc ccgcggccag cagcgccggt tcatcgctgt ggagcaggag
241 tttttccggc tgtggtggga tggcgtcgcc tcggaccagc agaaatacca ggtccgccag
301 ctcctggagg aaggacgcct ggaatttgtc atcggaggcc aggtcatgca tgacgaggct
361 gtgacgcacc ttgatgacca gatcctgcag ctcacagaag gacacgggtt tctctatgaa
421 acatttggga tccggccaca gttctcctgg cacgttgacc cgtttggcgc ctctgccacg
481 acgcccaccc tatttgcgct ggcgggcttc aatgcccacc tcggctcccg gatcgactac
541 gacctgaagg cagccatgca ggaggcccgg gggctgcagt tcgtgtggcg agggtcccca
601 tccctctcag agcggcagga aatcttcacg cacatcatgg accagtacag ctactgcacc
661 ccgtcccaca tccctttctc caacaggtca ggattttact ggaatggcgt ggctgtcttc
721 cccaagcctc ccccagatgg ggtgtacccc aacatgagtg agcctgtcac cccagccaac
781 atcaacctct atgccgaggc cctggtggcc aacgtgaagc agagggccgc ctggttccgg
841 acaccgcacg tcctctggcc ctggggatgt gacaagcagt tcttcaatgc ctcggtgcag
901 tttgccaaca tggacccgct gctggaccac atcaacagcc atgctgccga gctcggtgtc
961 tcggtgcagt atgccacgct gggcgactac ttccgtgccc tgcacgctct caatgtcacc
1021 tggcgtgtcc gcgaccacca cgacttcctg ccctattcca cagaaccatt ccaggcctgg
1081 acgggcttct acacgtcccg cagctcactg aaggggctgg cccggcgagc cagcgccttg
1141 ttgtatgccg gggagtccat gttcacacgc tacctgtggc cggccccccg tgggcatctg
1201 gaccccacct gggccctgca gcagctccag cagcttcgct gggccgtctc cgaggtccag
1261 caccatgatg ccatcactgg gactgagtcc cccaaggtga gagacatgta cgcaacgcac
1321 ctggcctcgg ggatgctggg cgtgcgcaag ctgatggcct ccatcgtcct agatgagctc
1381 cagccccagg cacccatggc ggccagctcc gatgcaggac ctgcaggaca ttttgcctcg
1441 gtctacaacc cgctggcctg gacggtcacc accatcgtca ccctgactgt tggtttccct
1501 ggagtccgcg tcacagatga ggcgggccac ccagtgccct cgcagatcca gaactcaaca
1561 gagaccccat ctgcgtatga cctgcttatt ctgaccacaa tcccaggcct cagttaccgg
1621 cactacagca tcagacccac tgcaggggcc caagagggca cccaggagcc ggctgccact
1681 gtggcgagca cccttcaatt tggccgcagg ctgaggagac gcaccagcca tgcgggcagg
1741 tacttggtgc ctgtggcaaa cgactgctac attgtgctgc tcgaccagga taccaacctg
1801 atgcacagca tctgggagag acagagtaac cgaacggtgc gcgtgaccca ggaattcctg
1861 gagtaccacg tcaacgggga tgtgaaacag ggccccattt ccgataacta cctgttcaca
1921 ccgggcaagg ccgcggtgcc tgcgtgggaa gctgtggaaa tggagattgt ggcgggacag
1981 cttgtgactg agatccggca gtacttctac aggaacatga cagcacagaa ttacacgtat
2041 gcaatccgct cccggctcac ccatgtgccg cagggccatg acggggagct gctctgccac
2101 cggatagagc aggagtacca agccggcccc ctggagctga accgtgaggc tgtcctgagg
2161 accagcacca acctaaacag ccagcaggtc atctactcag acaacaacgg ctaccagatg
2221 cagcggaggc cctacgtttc ctatgtgaac aacagcatcg cccggaatta ctaccccatg
2281 gttcagtcgg ccttcatgga ggatggcaaa agcaggcttg tgttgctgtc ggagcgggca
2341 catggcatct ccagccaagg gaatgggcag gtggaggtca tgctccaccg gcggctgtgg
2401 aacaacttcg actgggacct gggctacaac ctcacgctga acgacacctc agtcgtccac
2461 ccagtgctct ggcttctgct gggatcctgg tccctcacca ctgccctgcg ccagaggagc
2521 gcactggcgc tgcagcacag gcccgtggtg ctgttcggag acctcgctgg gactgcgccg
2581 aagctcccag gaccccagca gcaagaggcc gtgacgctgc ccccgaatct tcacctgcag
2641 atcctgagca tccctggctg gcgctacagc tccaaccaca cggagcactc tcagaatctc
2701 cggaaaggcc atcgagggga agcccaggct gacctccgcc gtgtcctgct gcggctctac
2761 cacctgtatg aagtgggcga ggacccagtc ctgtctcagc cagtaacagt gaatctggag
2821 gctgtgctgc aggcgctggg gtccgtggtg gcagtggagg agcgctcgct cacagggacc
2881 tgggatttga gcatgctgca ccgctggagc tggaggacgg ggcctggccg ccacagaggt
2941 gacaccacct ctccctcgag gccaccagga ggccccatca tcaccgtcca cccaaaggaa
3001 atccggacgt tctttattca ctttcaacag cagtgagccc tgggcagatg ccccgtcccc
3061 agggcttccc ccaggaactc catgtaacag aacagaccca ggacagggaa aagcagtgcg
3121 gagggatggg actggggagt cagctgctca tctgcaggct aatggcagga aatggtcata
3181 tttggggttt ttccctaatt tttttaaaca aaaattacat tacaagatcc aggttcttcc
3241 cccccacact caatcaagcc agccctctcc tcttctgtca cgtaaaggat atttggcaca
3301 ctcatgcgtc attcattcac aaaacacaaa cccaggactt tctgcctaag gcagaacaca
3361 agactcacag cagcaccgaa gcgcatctgc cgtccgggcc ctgccaggct tgccaggctg
3421 ccagtggtaa ctgtggacct actgcgtgcc acgtgttttc atagactcat cccatgctgg
3481 caacagccct gcaaggggct tggctctgcc acagggcagg agaggaagtt gtagcgccta
3541 gcgagagttc cagccccaga cgcccacctg tgcctcaggg caccgcctgc cgagcagaga
3601 aggcacagca gccgtcagag tccatgagag gtgaaaccac acagcaggga tgtccaatat
3661 cagaactatt aatatcaata aaagtataac cttcccaggt ctatgcccaa gagaattgaa
3721 aacatccatc cacacaatac ctgtgctccc gcgttcatag cagcattact caaaagtcaa
3781 acggtagcaa caacccaaat gtccatccac agatgaatta agacatgaag tgtgttctgt
3841 ccatacaatg gaatattatt tggccataaa aaggaaggaa attctgacgc atgccacagc
3901 ctgagtgaat cctacaaata ttacgctaag tgaaagaagc caatcacgag tttatgtgaa
3961 atgtccagaa taggcaaatc tgtgtatcag agacaaagca cattggtggt tgccaggtac
4021 tggaggaaga gagaagaggc atgacagcta acagggacgg gctttctttg gaagatgatg
4081 aaattgtgga atgatggttg cacaactttg tgaatatact agaaaccaat gaattaaaaa
4141 ctttggaaga tgaaaaaaaa aaaaaaaaaa aaaa
//