LOCUS BC032777 2458 bp mRNA linear HUM 06-MAR-2007
DEFINITION Homo sapiens actin filament associated protein 1, mRNA (cDNA clone
MGC:44840 IMAGE:4914388), complete cds.
ACCESSION BC032777
VERSION BC032777.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2458)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2458)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 68 Row: g Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 40807503.
FEATURES Location/Qualifiers
source 1..2458
/db_xref="H-InvDB:HIT000041714"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:44840 IMAGE:4914388"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2458
/gene="AFAP1"
/gene_synonym="AFAP"
/gene_synonym="AFAP-110"
/db_xref="GeneID:60312"
/db_xref="HGNC:HGNC:24017"
/db_xref="MIM:608252"
CDS 25..2217
/gene="AFAP1"
/gene_synonym="AFAP"
/gene_synonym="AFAP-110"
/codon_start=1
/product="actin filament associated protein 1"
/protein_id="AAH32777.1"
/db_xref="GeneID:60312"
/db_xref="HGNC:HGNC:24017"
/db_xref="MIM:608252"
/translation="MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGF
DVKDHAQKQETANSLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSP
GKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFL
LRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCNITYIPKDSKKKKHELK
ITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDSECPPPPSSPVHKAELEKKL
SSERPSSDGEGVVENGITTCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKIISLGKKK
PSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDNKLIFHKDRTDLKTHIVSIPL
RGCEVIPGLDCKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPEALH
YDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNGYAHPSGTALHYDDVPCIN
GSLKGKKPPVASNGVTGKGKTLSSQPKKADPAAVVKRTGSNAAQYKYGKNRVEADAKR
LQTKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKPQAILEEKLKQLEEECRQ
KEAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPIS
SCDTSDTEGPVPVNSAAVLKKSQAAPGSSPCRGHVLRKAKEWELKNGT"
BASE COUNT 661 a 650 c 703 g 444 t
ORIGIN
1 ccacgcgtcc gggcggcctg agcaatggaa gagttaatag ttgaacttcg tctctttctt
61 gaactcctgg accatgaata tctaacctca actgtcaggg agaaaaaggc agtgataacc
121 aacattctgc taagaataca gtcatccaaa ggttttgatg tgaaggacca tgctcagaag
181 caggagaccg ctaacagcct gccagcccct cctcagatgc ccctgccgga gatccctcag
241 ccctggctgc ctcctgacag tgggcctcca ccattgccaa catcctccct cccagaaggt
301 tattatgagg aagctgtgcc actgagcccc ggaaaagctc cggaatacat cacatcaaat
361 tatgattccg atgcgatgag cagctcttat gagtcgtatg atgaagagga ggaggatggg
421 aaggggaaga aaacccggca ccagtggccc tccgaggagg cctccatgga cctggtcaag
481 gacgccaaaa tctgcgcctt cctgctgcgg aagaagcggt tcggccagtg gaccaagttg
541 ctctgcgtca tcaaagacac caaactgctg tgctataaaa gttccaagga ccagcagcct
601 cagatggaac tgccactcca aggctgtaac attacgtaca tcccgaaaga cagcaaaaag
661 aagaagcacg agctgaagat tactcagcag ggcacggacc cgcttgttct cgccgtccag
721 agcaaggaac aggccgagca gtggctgaag gtgatcaaag aagcctacag tggttgtagt
781 ggccccgtgg attcagagtg tcctcctcca ccaagctccc cggtgcacaa ggcagaactg
841 gagaagaaac tgtcttcaga gagacccagc tcagatgggg agggtgttgt ggaaaatgga
901 attaccacat gtaatggaaa ggagcaagtg aagaggaaga aaagttccaa atcagaggcc
961 aagggcactg tgtcgaaagt cactgggaaa aaaatcacca agatcatcag tctgggaaag
1021 aaaaagccgt ccacagacga gcagacctcc tcagctgagg aagatgttcc cacctgcggc
1081 tatctgaacg tgctctccaa cagccgctgg cgagagcgct ggtgccgagt gaaagataac
1141 aagctcattt tccacaagga caggaccgac ctgaagaccc atattgtgtc tattccgctc
1201 cgtggctgcg aggtgatccc gggtttggat tgtaaacatc ctctgacgtt ccggctgctg
1261 cgcaacggcc aggaggttgc agtattggag gcatcttctt ctgaagacat gggcaggtgg
1321 attgggattt tactcgcaga gacgggatcg tccacagacc cggaggctct gcactatgac
1381 tacattgatg tggagatgtc tgcaagtgtc attcagacag ccaaacagac cttctgtttc
1441 atgaacaggc gtgttatatc tgctaaccca tatctagggg gcacctccaa cggctatgcc
1501 caccccagcg ggacggcact tcattatgac gatgtcccgt gcatcaacgg ctcgctcaag
1561 ggtaaaaagc cccccgtggc gtctaatggg gtcacaggaa aagggaagac tctgagcagt
1621 cagccaaaga aagcggatcc cgcggctgtt gtgaaaagga cgggttcgaa tgctgcccag
1681 tacaagtatg gcaagaaccg ggtagaagca gatgccaagc ggctacagac caaagaggag
1741 gagctgctga agaggaaaga ggccctgcgg aataggctgg cccagctccg caaggaaaga
1801 aaagaccttc gagcggctat tgaagtgaac gccggcagga agccgcaggc gatcctggag
1861 gagaagctga agcagctgga ggaggagtgc cggcagaagg aggcggagcg tgtcagcctg
1921 gagctggagc tgacggaggt caaggagagc ctgaagaaag cgctggcggg cggagtcacc
1981 ctggggctgg ccatcgagcc caagtcaggg acatcgagtc cacagtctcc agtgttccgg
2041 caccggaccc tggaaaactc gcccatctcc agctgtgaca ccagtgacac cgagggcccc
2101 gtgccggtga acagcgcggc cgtcttgaag aagagccagg ctgccccggg cagctccccc
2161 tgccgagggc atgtgctgcg gaaggccaag gaatgggaat tgaagaacgg gacctagggg
2221 acagcagcac cactccagcc tcagagactg cacaccccct tgcctgtatc ctcatctgtg
2281 tgacggcagg aagctctgcc cagagtggcc tcagctgcac gactccagag gctccacgac
2341 tgagctctga ggccagtgcc tgtcccccag gcccacttgt attctttcta ctgtaaaatg
2401 gcgcctttaa aaaagatgta agactttggt tcccaacttt gttaaaaaaa aaaaaaaa
//